Abstract: Disclosed is a method for producing a fusion protein in which an antibody and a lysosomal enzyme are fused. The method is a method for producing a fusion protein in which an antibody and a human lysosomal enzyme are fused, the method including: (a) culturing mammalian cells producing the fusion protein in a serum-free medium to secrete the fusion protein in a culture medium; (b) collecting a culture supernatant by removing the mammalian cells from the culture medium; and (c) purifying the fusion protein from the culture supernatant by using a column chromatography using a material to which a substance having affinity for the antibody is bound as a solid phase, an anion exchange column chromatography, a cation exchange column chromatography, and a size exclusion column chromatography.

Abstract: There is provided a treatment method that is capable of reducing burden on a patient when a medical sheet is indwelled in the body of the patient. A treatment method includes an introduction of introducing a catheter, which has stored a medical sheet (e.g., a myocardial cell sheet), to a heart inside a living body, and an indwelling step of drawing the myocardial cell sheet from the catheter and indwelling the myocardial cell sheet in the heart.

Abstract: There is provided a treatment method that is capable of reducing burden on a patient when a medical sheet is indwelled in the body of the patient. A treatment method includes an introduction of introducing a catheter, which has stored a medical sheet (e.g., a myocardial cell sheet), to a heart inside a living body, and an indwelling step of drawing the myocardial cell sheet from the catheter and indwelling the myocardial cell sheet in the heart.

Abstract: A catheter balloon and a balloon catheter in which compliance characteristics of a membrane are improved in its entirety. The catheter balloon includes a membranous body that can dilate and contract by fluid supplied from a catheter. The membranous body includes an intermediate layer which is formed of a non-elastomer, an outer layer which is arranged on an outer surface of the intermediate layer and contains an elastomer, and an inner layer which is arranged on an inner surface of the intermediate layer and contains an elastomer. The average wall thickness of the intermediate layer is within 30% to 70% of the wall thickness of the balloon in its entirety.