Abstract: Autoinflammatory and mitochondrial disorders can be treated by positioning a plurality of electrodes in or on a subject's body, and applying an AC voltage between the plurality of electrodes so as to impose an alternating electric field through the tissue that is being affected by the autoinflammatory or mitochondrial disease. The frequency and field strength of the alternating electric field are selected such that the alternating electric field inhibits inflammation or mitochondrial disorders in the tissue.

Abstract: Compositions, systems, and methods for reducing electrosensation and/or skin irritation in response to the application of alternating electric fields to a skin of a subject are disclosed. The compositions, systems, and methods involve administration of at least one composition comprising at least one localized numbing agent to the subject followed by application of an alternating electric field to the subject.

Abstract: Immunogenic compositions and systems are disclosed, as well as methods for producing the immunogenic compositions. Compositions, systems, and methods of reducing viability of cancer cells and treating cancer, as well as methods of reducing volume of a tumor and/or preventing an increase in volume of a tumor present in a body of a living subject, are also disclosed. The systems and methods involve application of an alternating electric field to cancer cell(s) (before or after isolation from a targeted portion of a subject), and irradiation of the isolated and treated cancer cell(s). The methods may further include administration of the irradiated, treated cancer cells back to the subject from which the cancer cell(s) were isolated, and/or application of an alternating electric field to the subject. The immunogenic compositions comprise populations of isolated, apoptotic cancer cells.

Abstract: Compositions, systems, and methods for activating dendritic cells are disclosed. Also disclosed are compositions, systems, and methods for reducing viability of cancer cells and treating cancer, as well as preventing an increase in volume of a tumor present in a body of a living subject, along with methods of treating other diseases and infections. The systems and methods involve application of an alternating electric field to dendritic cell(s) in vivo or ex vivo and/or to a subject or cancer cells isolated therefrom. The systems and methods may further include administration of activated dendritic cells to a subject. The compositions comprise populations of isolated dendritic cells activated by exposure to an alternating electric field.

Abstract: A method for treating a subject, the method comprises delivering an interleukin 23 (IL-23) inhibitor to the subject and applying an alternating electric field to the subject at a frequency between approximately 50 kHz and approximately 10,000 kHz.

Abstract: Disclosed are methods and compositions for preventing or disrupting mitosis of pluripotent stem cells, killing pluripotent stem cells, preventing or disrupting division of pluripotent stem cells, reducing the viability of pluripotent stem cells, slowing the progression or differentiation of pluripotent stem cells, and treating an ectopic pregnancy.

Abstract: Tumor treating fields (TTFields) can be delivered by implanting a plurality of sets of implantable electrode elements within a person's body. Temperature sensors positioned to measure the temperature at the electrode elements are also implanted, along with a circuit that collects temperature measurements from the temperature sensors. In some embodiments, an AC voltage generator configured to apply an AC voltage across the plurality of sets of electrode elements is also implanted within the person's body.

Abstract: Tumor treating fields (TTFields) can be delivered by implanting a plurality of sets of implantable electrode elements within a person's body. Temperature sensors positioned to measure the temperature at the electrode elements are also implanted, along with a circuit that collects temperature measurements from the temperature sensors. In some embodiments, an AC voltage generator configured to apply an AC voltage across the plurality of sets of electrode elements is also implanted within the person's body.

Abstract: Disclosed are methods of treating that can be performed using alternating electric fields applied in a single direction or multiple directions. Disclosed are method of treating a subject having diabetes comprising applying alternating electric fields to a target site of the subject for a period of time, the alternating electric fields having a frequency and field strength, wherein FGF21 is increased in the subject.

Abstract: Disclosed are methods and compositions for preventing or disrupting mitosis of pluripotent stem cells, killing pluripotent stem cells, preventing or disrupting division of pluripotent stem cells, reducing the viability of pluripotent stem cells, slowing the progression or differentiation of pluripotent stem cells, and treating an ectopic pregnancy.

Abstract: Systems and methods are disclosed for inducing apoptosis and treating or reducing the occurrence of at least one condition, disease, disorder, or infection in a subject. The systems and methods rely on the application of an alternating electric field and administration of a clustered regularly interspaced short palindromic repeats (CRISPR) associated protein (CRISPR-Cas) system. Also provided are kits for performing the methods disclosed herein.

Abstract: Compositions, systems, and methods for reducing viability of cancer cells and treating cancer, as well as preventing an increase of volume of a tumor present in a body of a living subject, are disclosed. The systems and methods involve application of an alternating field in combination with administration of at least one composition comprising at least one small molecule anti-angiogenic agent that specifically interacts with VEGF.

Abstract: Disclosed are methods of treating a subject in need thereof comprising: applying alternating electric fields, at a frequency for a period of time, to a target site of the subject in need thereof; and administering one or more FGF inhibitors or FGFR inhibitors to the subject in need thereof. Disclosed are methods of increasing a cell's sensitivity to alternating electric fields comprising: applying alternating electric fields, at a frequency for a period of time, to a cell; and contacting one or more FGF inhibitors or FGFR inhibitors to the cell, thereby increasing the cell's sensitivity to the alternating electric fields. Disclosed are methods of increasing cytotoxicity in a cell comprising: applying alternating electric fields, at a frequency for a period of time, to a cell; and contacting one or more FGF inhibitors or FGFR inhibitors to the cell, thereby increasing cytotoxicity in the cell.

Abstract: A method of treating a subject, the method comprises applying an alternating electric field focused on a liver of the subject at a frequency between approximately 50 kHz and approximately 10,000 kHz, where the subject has hormonal disorder and/or polycystic ovary syndrome.

Abstract: Systems and methods for increasing absorption of ingested substances into a subject's bloodstream are disclosed. The systems and methods involve application of an alternating field to the subject's abdomen, and may further include oral administration of at least one substance to the subject. Also disclosed are methods of treating or reducing the occurrence of a malabsorption condition in a subject via application of the alternating electric field to the subject's abdomen.

Abstract: Disclosed are methods of altering the electric impedance to an alternating electric field in a target site of a subject, comprising introducing a nanoparticle to a target site in the subject; and applying an alternating electric field to the target site of the subject, wherein the electric impedance in the target site of the subject to the alternating current is altered. Disclosed are methods for improving transport of a nanoparticle across a cell membrane of a cell, the method comprising applying an alternating electric field to the cell for a period of time, wherein application of the alternating electric field increases permeability of the cell membrane; and introducing the nanoparticle to the cell, wherein the increased permeability of the cell membrane enables the nanoparticle to cross the cell membrane.

Abstract: Alternating electric fields (e.g., TTFields) may be induced in a target region in a subject's body by applying, during each of a plurality of first time intervals, a series of pulses of alternating current between electrode elements positioned on or in the subject's body. Immediately following each first interval of time, the electrode elements are allowed to cool. Although the pulses of alternating current within any given first time interval have amplitudes at a level that would cause overheating if the series of pulses was allowed to continue for one hour, each series of pulses does not, in fact, continue for one hour. To the contrary, each series of pulses is short enough to avoid overheating. Interleaving the cooling periods between the pulsing periods enables higher-current pulses to be used, and the use of those higher-current pulses can advantageously improve the efficacy of treatment.

Abstract: Cancer cells can be synchronized to the G2/M phase by delivering an anti-microtubule agent (e.g., paclitaxel or another taxane) to the cancer cells, and applying an alternating electric field with a frequency between 100 and 500 kHz to the cancer cells, wherein at least a portion of the applying step is performed simultaneously with at least a portion of the delivering step. This synchronization can be taken advantage of by treating the cancer cells with radiation therapy after the combined action of the delivering step and the applying step has increased a proportion of cancer cells that are in the G2/M phase. The optimal frequency and field strength will depend on the particular type of cancer cell being treated. For certain cancers, this frequency will be between 125 and 250 kHz (e.g., 200 kHz) and the field strength will be at least 1 V/cm.

Abstract: Tumor treating fields (TTFields) can be delivered by implanting a plurality of sets of implantable electrode elements within a person’s body. Temperature sensors positioned to measure the temperature at the electrode elements are also implanted, along with a circuit that collects temperature measurements from the temperature sensors. In some embodiments, an AC voltage generator configured to apply an AC voltage across the plurality of sets of electrode elements is also implanted within the person’s body.

Abstract: Viral infections in a target region can be inhibited by imposing an alternating electric field in the target region for a duration of time. The alternating electric field has a frequency and a field strength such that when the alternating electric field is imposed in the target region for the duration of time, the alternating electric field inhibits infection of the cells in the target region by the virus. Optionally, the inhibition of viral infections using the alternating electric field approach can be combined with delivering an antiviral agent to the target region so that a therapeutically effective dose of the antiviral agent is present in the target region while the alternating electric fields are imposed.