Patents by Inventor Tatsuji Enoki

Tatsuji Enoki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20150291980
    Abstract: According to the present invention, an AAV vector having a higher titer compared with those of conventional ones can be produced using a cell into which a nucleic acid capable of expressing miRNA is introduced artificially. An AAV vector produced using the cell and a composition containing the viral vector as an active ingredient are very useful as gene transfer means in the studies or clinical practice of gene therapies.
    Type: Application
    Filed: June 26, 2013
    Publication date: October 15, 2015
    Applicant: TAKARA BIO INC.
    Inventors: Toshikazu Nishie, Fuyuko Takashima, Tatsuji Enoki, Junichi Mineno
  • Patent number: 9062287
    Abstract: A process for producing a cell mass containing natural killer cells, characterized by involving a step of carrying out the expansion culture of a cell mass containing natural killer cells and/or cells capable of being differentiated into natural killer cells in the presence of a biological response modifier and cells that has been so treated as to lose a proliferation capability, and others.
    Type: Grant
    Filed: September 10, 2010
    Date of Patent: June 23, 2015
    Assignee: TAKARA BIO INC.
    Inventors: Mitsuko Ideno, Mie Yabuuchi, Meiko Jin, Masae Sato, Naoko Ashida, Tatsuji Enoki
  • Patent number: 8927273
    Abstract: The present invention provides a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one step selected from induction, maintenance and expansion of a cytotoxic lymphocyte using a medium containing serum and plasma at a total concentration of 0% by volume or more and less than 5% by volume, in the presence of fibronectin, a fragment thereof or a mixture thereof.
    Type: Grant
    Filed: August 19, 2004
    Date of Patent: January 6, 2015
    Assignee: Takara Bio Inc.
    Inventors: Mitsuko Ideno, Nobuko Muraki, Kinuko Ogawa, Masayuki Kishimoto, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • Patent number: 8835177
    Abstract: A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.
    Type: Grant
    Filed: June 12, 2006
    Date of Patent: September 16, 2014
    Assignee: Takara Bio Inc.
    Inventors: Takahiro Marui, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • Patent number: 8765469
    Abstract: A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: July 1, 2014
    Assignee: Takara Bio Inc.
    Inventors: Takahiro Marui, Kinuko Nagamine, Nobuko Muraki, Akiko Kato, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • Patent number: 8216837
    Abstract: A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.
    Type: Grant
    Filed: June 7, 2007
    Date of Patent: July 10, 2012
    Assignee: Takara Bio Inc.
    Inventors: Nobuko Muraki, Mitsuko Ideno, Kinuko Nagamine, Fuyuko Takashima, Eiji Kobayashi, Akiko Kato, Takahiro Marui, Hiroaki Sagawa, Tatsuji Enoki, Ikunoshin Kato
  • Publication number: 20120171173
    Abstract: A process for producing a cell mass containing natural killer cells, characterized by involving a step of carrying out the expansion culture of a cell mass containing natural killer cells and/or cells capable of being differentiated into natural killer cells in the presence of a biological response modifier and cells that has been so treated as to lose a proliferation capability, and others.
    Type: Application
    Filed: September 10, 2010
    Publication date: July 5, 2012
    Inventors: Mitsuko Ideno, Mie Yabuuchi, Meiko Jin, Masae Sato, Naoko Ashida, Tatsuji Enoki
  • Publication number: 20120149108
    Abstract: A method for cryopreservation of cells characterized by the use of a solution containing, as essential components, a sodium salt, a potassium salt, a saccharide, a cryoprotectant, and a hydrogencarbonate salt and/or a carbonate salt, the solution further optionally containing a component selected from the group consisting of proteins, magnesium salts, and calcium salts. According to the present invention, a method for cryopreservation of cells capable of maintaining a high viability rate after thawing, and a cryopreservation solution used in the cryopreservation are provided. In addition, by using the method for cryopreservation, the cells can be preserved in a state of maintaining a high viability rate even after thawing, and also maintaining their physiological functions; further, a cell washing step after freezing and thawing is unnecessary, thereby making it very useful in the fundamental researches of cells and the application studies on the medical treatment.
    Type: Application
    Filed: August 17, 2010
    Publication date: June 14, 2012
    Inventors: Masashige Tanabe, Tomomi Isono, Tatsuji Enoki
  • Publication number: 20110158954
    Abstract: A method for preparing a ?? T cell population, characterized in that the method includes the step of culturing a cell population containing ?? T cells, in the presence of (a) fibronectin, a fibronectin fragment or a mixture thereof and (b) an activating factor of ?? T cells. According to the present invention, a method for preparing a ?? T cell population is provided. The ?? T cell population obtained by the method is suitably used in, for example, immunotherapy. Therefore, the method of the present invention is expected to greatly contribute to a medical field.
    Type: Application
    Filed: March 4, 2008
    Publication date: June 30, 2011
    Inventors: Mitsuko Ideno, Fumiyo Sakai, Tatsuji Enoki, Ikunoshin Kato
  • Publication number: 20110117653
    Abstract: The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.
    Type: Application
    Filed: July 7, 2009
    Publication date: May 19, 2011
    Applicant: Takara Bio Inc.
    Inventors: Tatsuji Enoki, Fumiko Iwamoto, Toshikazu Nishie, Takahiro Marui, Fuyuko Takashima, Ikunoshin K
  • Publication number: 20100317613
    Abstract: It is intended to provide a drug, a food or a feed which has an effect of enhancing the activity of a second-phase detoxifying enzyme and an effect of increasing intracellular glutathione content.
    Type: Application
    Filed: October 11, 2007
    Publication date: December 16, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Hiromu Ohnogi, Yoko Kudo, Hiroko Nakahara, Tatsuji Enoki, Ikunoshin Kato
  • Publication number: 20100255578
    Abstract: A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.
    Type: Application
    Filed: June 7, 2007
    Publication date: October 7, 2010
    Inventors: Nobuko Muraki, Mitsuko Ideno, Kinuko Nagamine, Fuyuko Takashima, Eiji Kobayashi, Akiko Kato, Takahiro Marui, Hiroaki Sagawa, Tatsuji Enoki, Ikunoshin Kato
  • Publication number: 20100247579
    Abstract: The present invention relates to a method for treatment of cancer, which comprises the following steps (A) and (B): (A) applying a treatment which induces the reduction in lymphocytes to a patient; and (B), subsequent to step (A), administering lymphocytes to the patient promptly. The present invention also relates to a cancer therapeutic agent and a cancer treatment kit for use in the method. When the method is used as an immunoreconstructive therapy, the decrease in immunologic competence which may be caused by the reduction in lymphocytes can be avoided and, therefore, the risk of the occurrence of an infectious disease can be decreased.
    Type: Application
    Filed: May 8, 2008
    Publication date: September 30, 2010
    Inventors: Hiroshi Shiku, Shinichi Kageyama, Shigehisa Kitano, Mitsuko Ideno, Keisuke Tomita, Tatsuji Enoki, Kazutoh Takesako, Ikunoshin Kato
  • Publication number: 20100150886
    Abstract: A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.
    Type: Application
    Filed: August 10, 2006
    Publication date: June 17, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Takahiro Marui, Kinuko Magamine, Nobuke Muraki, Akiko Kato, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • Publication number: 20100137228
    Abstract: Medicinal compositions for treating, ameliorating or preventing diseases with sensitivity to 3,6-anhydrogalactopyranose represented by formula (1): foods, drinks, cosmetics, etc. containing as the active ingredient at least one member selected from the group consisting of the above-mentioned compound, its aldehyde, its hydrate and 2-O-methylated derivatives thereof and soluble sugar compounds containing the above compound. This compound also shows, for example, an apoptosis-inducing activity, a carcinostatic activity and inhibitory activities on the production of active oxygen, lipid peroxide radicals and NO, which makes it useful also as the active ingredient of antioxidants and preservatives.
    Type: Application
    Filed: August 11, 2008
    Publication date: June 3, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Tatsuji ENOKI, Hiroaki SAGAWA, Takanari TOMINAGA, Eiji NISHIYAMA, Nobuto KOYAMA, Takeshi SAKAI, Fu-Gong YU, Katsushige IKAI, Ikunoshin KATO
  • Publication number: 20100068192
    Abstract: A method for preparing a T cell population, wherein the T cell population expresses CD45RA and expresses at least one selected from the group consisting of CD62L, CCR7, CD27, and CD28, characterized in that the method comprises the step of culturing a cell population comprising a T cell, in the presence of fibronectin, a fragment thereof or a mixture thereof.
    Type: Application
    Filed: September 27, 2006
    Publication date: March 18, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Tatsuji Enoki, Akiko Kato, Mitsuko Ideno, Hiroaki Sagawa, Ikunoshin Kato
  • Publication number: 20100048491
    Abstract: Medicinal compositions for treating, ameliorating or preventing diseases with sensitivity to 3,6-anhydrogalactopyranose represented by formula (1): foods, drinks, cosmetics, etc. containing as the active ingredient at least one member selected from the group consisting of the above-mentioned compound, its aldehyde, its hydrate and 2-O-methylated derivatives thereof and soluble sugar compounds containing the above compound. This compound also shows, for example, an apoptosis-inducing activity, a carcinostatic activity and inhibitory activities on the production of active oxygen, lipid peroxide radicals and NO, which makes it useful also as the active ingredient of antioxidants and preservatives.
    Type: Application
    Filed: August 11, 2008
    Publication date: February 25, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Tatsuji ENOKI, Hiroaki SAGAWA, Takanari TOMINAGA, Eiji NISHIYAMA, Nobuto KOYAMA, Takeshi SAKAI, Fu-Gong YU, Katsushige IKAI, Ikunoshin KATO
  • Publication number: 20090292012
    Abstract: A therapeutic agent or prophylactic agent for a disease requiring an anti-foaming action for a cell and/or an inhibitory action for acyl-CoA cholesterol acyltransferase in treatment or prevention, characterized in that the therapeutic agent or prophylactic agent comprises as an effective ingredient a processed product derived from Peucedanum japonicum.
    Type: Application
    Filed: January 26, 2006
    Publication date: November 26, 2009
    Inventors: Tatsuji Enoki, Yoko Kudo, Katsumi Sugiyama, Hiromu Ohnogi, Hiroaki Sagawa, Ikunoshin Kato
  • Publication number: 20090221077
    Abstract: The present invention provides a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one step selected from induction, maintenance and expansion of a cytotoxic lymphocyte using a medium containing serum and plasma at a total concentration of 0% by volume or more and less than 5% by volume, in the presence of fibronectin, a fragment thereof or a mixture thereof.
    Type: Application
    Filed: August 19, 2004
    Publication date: September 3, 2009
    Inventors: Mitsuko Ideno, Nobuko Muraki, Kinuko Ogawa, Masayuki Kishimoto, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • Publication number: 20090162936
    Abstract: A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.
    Type: Application
    Filed: June 12, 2006
    Publication date: June 25, 2009
    Applicant: TAKARA BIO. INC.
    Inventors: Takahiro Marui, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato