Abstract: A method producing a non-heme haloperoxidase which is substantially resistant to inactivation, at room temperature, in up to 0.3M H.sub.2 O.sub.2 for up to 25 hours, and up to 0.5mM HOCl for up to two minutes. One such haloperoxidase, isolated from Curvularia inaequalis, contains about 2 gram atoms of zinc per molecule. A halogenation reaction employing the enzyme can be performed at H.sub.2 O.sub.2 and hypohalous acid concentrations which produce rapid inactivation of heme-containing haloperoxidases.

Abstract: Surface polysaccharide and somatic polysaccharide are recovered from gram-negative nonenteric pathogenic bacilli in purified forms that are useful as veterinary acellular vaccines either individually or combined. The surface polysaccharide recovery process involves shearing the surface polysaccharide and somatic polysaccharide from the cell membrane, removing the bacteria, isolating the surface polysaccharide by precipitation with Cetavlon, solubilizing the precipitate, and removing nucleic acids by ethanol precipitation. The somatic polysaccharide is recovered from the supernatant remaining after the Cetavlon precipitation by ethanol precipitation.

Abstract: A method of producing a non-heme haloperoxidase which is substantially resistant to inactivation, at room temperature, in up to 0.3M H.sub.2 O.sub.2 for up to 25 hours, and up to 0.5 mM HOCl for up to two minutes. One such haloperoxidase, isolated from Curvularia inaequalis, contains about 2 gram atoms of zinc per molecule. A halogenation reaction employing the enzyme can be performed at H.sub.2 O.sub.2 and hypohalous acid concentrations which produce rapid inactivation of heme-containing haloperoxidases.