Abstract: The invention has an object to provide a method of making an optical fiber array in which bare fibers obtained by removing a coating of an optical fiber ribbon can be certainly arrayed on a V-groove substrate. A first feature of the invention is to use a positioning guide, and the positioning guide is provided with a recess portion having inclined wall surfaces, and its bottom portion is made to have a width equal to the whole width of the bare fibers in an arrayed state. By using this positioning guide, the optical fiber array can be made in a process as follows. In a state where bare fibers exposed by removing part of coating of tip portions of two fiber ribbons are alternately arranged, the positioning guide is raised from below. The bare fibers with irregular gaps are moved by the positioning guide and are arranged. In this state, the bare fibers are pressed by a fiber pressing member from above, and the V-groove substrate is raised, so that they are put on V grooves and are fixed by an adhesive.