Patents by Inventor Teruhide Sugisawa
Teruhide Sugisawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7670814Abstract: The present invention provides a process for the production of vitamin C from a substrate, such as for instance L-sorbosone using a microorganism belonging to the genus Ketogulonicigenium.Type: GrantFiled: January 22, 2005Date of Patent: March 2, 2010Assignee: DSM IP Assets B.V.Inventors: Tatsuo Hoshino, Teruhide Sugisawa
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Patent number: 7544494Abstract: The present invention relates to a process for the production of vitamin C from L sorbosone using an aldehyde dehydrogenase which is isolated from Gluconobacter oxydans DSM 4025 (FERM BP-3812), said enzyme having the following physicochemical properties: (a) molecular weight of 150,000±6,000 Da or 230,000±9,000 Da (consisting of 2 or 3 homologous subunits, each subunit having a molecular weight of 75,000±3,000 Da); (b) substrate specificity as active on aldehyde compounds; (c) Cofactors are pyrroloquinoline quinone and heme c; (d) Optimum pH between 6.4 and 8.2 for vitamin C production from L-sorbosone; and (e) as inhibitors Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and ethylendiamine tetraacetic acid. The process is performed in the presence of a suitable electron acceptor and the vitamin C isolated from the reaction mixture.Type: GrantFiled: September 22, 2003Date of Patent: June 9, 2009Assignee: DSM IP Assets B.V.Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Patent number: 7341854Abstract: The present invention provides a process for the production of vitamin C from different substrates like D-sorbitol, L-sorbose, L-sorbosone or L-gulose using a microorganism selected from the group consisting of Gluconobacter oxydans DMS 4025 (FERM BP-3812), a microorganism belonging to the genus Gluconobacter and having identifying characteristics of G. oxydans DSM 4025 (FERM BP-3812) and mutants thereof.Type: GrantFiled: September 22, 2003Date of Patent: March 11, 2008Assignee: DSM IP Assets B.V.Inventors: Tatsuo Hoshino, Teruhide Sugisawa
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Publication number: 20070161093Abstract: The present invention provides a process for the production of vitamin C from a substrate, such as for instance L-sorbosone using a microorganism belonging to the genus Ketogulonicigenium.Type: ApplicationFiled: January 22, 2005Publication date: July 12, 2007Inventors: Tatsuo Hoshino, Teruhide Sugisawa
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Patent number: 7135315Abstract: The present invention relates to a novel DNA which encodes aldehyde dehydrogenase (SNDH), an expression vector containing the DNA and recombinant organisms containing said DNA. Furthermore, the present invention concerns a process for producing recombinant aldehyde dehydrogenase protein and a process for producing L-ascorbic acid (vitamin C) and/or 2-keto-L-gulonic acid (2-KGA) from L-sorbosone by using the recombinant aldehyde dehydrogenase protein or recombinant organisms containing the expression vector. Also provided is a process for the production of 2KGA with a microorganism in which the gene encoding said aldehyde dehydrogenase is disrupted.Type: GrantFiled: September 22, 2003Date of Patent: November 14, 2006Assignee: DSM IP Assets B.V.Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Publication number: 20060127994Abstract: The present invention relates to a novel DNA which encodes aldehyde dehydrogenase (SNDH), an expression vector containing the DNA and recombinant organisms containing said DNA. Furthermore, the present invention concerns a process for producing recombinant aldehyde dehydrogenase protein and a process for producing L-ascorbic acid (vitamin C) and/or 2-keto-L-gulonic acid (2-KGA) from L-sorbosone by using the recombinant aldehyde dehydrogenase protein or recombinant organisms containing the expression vector. Also provided is a process for the production of 2KGA with a microorganism in which the gene encoding said aldehyde dehydrogenase is disrupted.Type: ApplicationFiled: September 22, 2003Publication date: June 15, 2006Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Publication number: 20060121582Abstract: The present invention relates to a process for the production of vitamin C from L sorbosone using an aldehyde dehydrogenase which is isolated from Gluconobacter oxydans DSM 4025 (FERM BP-3812), said enzyme having the following physicochemical properties: (a) molecular weight of 150,000±6,000 Da or 230,000±9,000 Da (consisting of 2 or 3 homologous subunits, each subunit having a molecular weight of 75,000±3,000 Da); (b) substrate specificity as active on aldehyde compounds; (c) Cofactors are pyrroloquinoline quinone and heme c; (d) Optimum pH between 6.4 and 8.2 for vitamin C production from L-sorbosone; and (e) as inhibitors Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and ethylendiamine tetraacetic acid. The process is performed in the presence of a suitable electron acceptor and the vitamin C isolated from the reaction mixture.Type: ApplicationFiled: September 22, 2003Publication date: June 8, 2006Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Publication number: 20060035349Abstract: The present invention provides a process for the production of vitamin C from different substrates like D-sorbitol, L-sorbose, L-sorbosone or L-gulose using a microorganism selected from the group consisting of Gluconobacter oxydans DSM 4025 (FERM BP-3812), a microorganism belonging to the genus Gluconobacter and having identifying characteristics of G. oxydans DSM 4025 (FERM BP-3812) and mutants thereof.Type: ApplicationFiled: September 22, 2003Publication date: February 16, 2006Inventors: Tatsuo Hoshino, Teruhide Sugisawa
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Publication number: 20050153412Abstract: The present invention concerns a novel aldehyde dehydrogenase having the following physico-chemical properties: a molecular weight of 100,000±10,000 Da which comprises two homologous subunits or a molecular weight of 150,000±15,000 Da which comprises three homologous subunits, each subunit having a molecular weight of 55,000±2,000 Da; dehydrogenase activity on L-sorbosone, D-Glucosone, D-glucose and D-xylose; utilizes as cofactor pyrroloquinoline quinone; has an optimum pH of from 6.5 to 8.0 for the production of vitamin C and an optimum pH of about 9.0 for the production of 2-keto-L-gulonic acid from L-sorbosone; and is inhibited by Co2+, Cu2+, Fe3+, Ni2+, Zn2+, and monoiodoacetate, is derived from a microorganism belonging to the genus Gluconobacter.Type: ApplicationFiled: April 14, 2003Publication date: July 14, 2005Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Patent number: 6846660Abstract: A new aldehyde dehydrogenase having the physico-chemical properties: molecular weight:150,000±6,000 or 230,000±9,000; substrate specificity active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c; optimum pH: 7.0-8.5; and inhibitors: Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and EDTA, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism.Type: GrantFiled: March 23, 2001Date of Patent: January 25, 2005Assignee: Roche Vitamins Inc.Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Publication number: 20010026933Abstract: A new aldehyde dehydrogenase having the physico-chemical properties:molecular weight: 150,000±6,000 or 230,000±9,000; substrate specificity:active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c; optimum pH: 7.0-8.5; and inhibitors: Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and EDTA, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism.Type: ApplicationFiled: March 23, 2001Publication date: October 4, 2001Applicant: ROCHE VITAMINS INC.Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Patent number: 6242233Abstract: A new aldehyde dehydrogenase having the physico-chemical properties: molecular weight: 150,000±6,000 or 230,000±9,000; substrate specificity:active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c; optimum pH: 7.0-8.5; and inhibitors: Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and EDTA, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism.Type: GrantFiled: December 1, 1998Date of Patent: June 5, 2001Assignee: Roche Vitamins Inc.Inventors: Tatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
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Patent number: 5776742Abstract: An aldehyde dehydrogenase having the physico-chemical properties: molecular weight: 91,000.+-.5,000; substrate specificity:active on aldehyde compounds; inhibition: by Cu.sup.2+, Zn.sup.2+, Ni.sup.2 + and ethylenediamine tetraacetic acid; optimum pH: 6.0-8.5; optimum temperature: 20.degree.-40.degree. C.; and stimulator: Ca.sup.2+ and pyrroloquinoline quinone, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism.Type: GrantFiled: February 6, 1997Date of Patent: July 7, 1998Assignee: Roche Vitamins Inc.Inventors: Tatsuo Hoshino, Teruhide Sugisawa
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Patent number: 5747301Abstract: A D-sorbitol dehydrogenase in homogenous form, which catalyzes the oxidation of D-sorbitol to L-sorbose, has a molecular structure consisting of homologous subunits of molecular weight 79,000.+-.5,000 each, a substrate specificity for polyols and an optimum pH of 6.0-7.0. Said D-sorbitol dehydrogenase is producible by a process comprising cultivating a microorganism belonging to the genus Gluconobacter or Acetobacter, or a mutant or variant thereof, which is capable of producing the D-sorbitol dehydrogenase in the cells and isolating it from the cells, e.g. by disrupting the cells and isolation from cell-free extract of the disrupted cells. The so-isolated D-sorbitol dehydrogenase is useful for catalyzing the oxidation of D-sorbitol to L-sorbose, the latter being an important intermediate for the production of vitamin C.Type: GrantFiled: February 26, 1996Date of Patent: May 5, 1998Assignee: Roche Vitamins Inc.Inventors: Tatsuo Hoshino, Setsuko Ojima, Teruhide Sugisawa
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Patent number: 5541108Abstract: A process for producing 2-keto-L-gulonic acid which comprises converting L-sorbose and/or D-sorbitol into 2-keto-L-gulonic acid with the aid of a microorganism or its cell free extract, said microorganism belonging to the species Gluconobacter oxydans capable of producing 2-keto-L-gulonic acid and having L-sorbose dehydrogenase activity. Also disclosed are specific microorganisms useful in such process.Type: GrantFiled: June 28, 1994Date of Patent: July 30, 1996Assignee: Hoffmann-La Roche Inc.Inventors: Akiko Fujiwara, Teruhide Sugisawa, Masako Shinjoh, Yutaka Setoguchi, Tatsuo Hoshino
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Patent number: 5312741Abstract: The present invention relates to a process for producing 2-keto-L-gulonic acid by fermentative conversion of D-sorbitol in high yield. 2-Keto-L-gulonic acid is an important intermediate for the production of L-ascorbic acid into which it can be converted.Type: GrantFiled: June 8, 1992Date of Patent: May 17, 1994Assignee: Hoffmann-La Roche Inc.Inventors: Tatsuo Hoshino, Setuko Ojima, Teruhide Sugisawa
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Patent number: 5250428Abstract: The present invention concerns a novel process for the microbiological manufacture of L-ascorbic acid from L-gulono-gamma-lactone, a process for preparing the enzyme being responsible for the microbiological oxidation of L-gulono-gamma-lactone, and said enzyme as a homogeneous protein, which is a L-gulono-gamma-lactone dehydrogenase.Type: GrantFiled: September 17, 1991Date of Patent: October 5, 1993Assignee: Hoffmann-La Roche Inc.Inventors: Tatsuo Hoshino, Peter K. Matzinger, Setsuko Ojima, Teruhide Sugisawa
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Patent number: 4960695Abstract: A process of producing 2-keto-L-gulonic acid by fermentative conversion of L-sorbose utilizing a fermentation system composed of component produced from a microorganism having the identifying characteristics of strain DSM No. 4025 and a yeast component.Type: GrantFiled: September 26, 1989Date of Patent: October 2, 1990Assignee: Hoffmann-La Roche Inc.Inventors: Tatsuo Hoshino, Setsuko Nomura, Teruhide Sugisawa
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Patent number: 4916069Abstract: The present invention relates to novel coenyme independent L-sorbose dehydrogenase and a process for producing the same. The enzyme provided by the present invention catalyzes the oxidation of L-sorbose to L-sorbosone, the precursor of 2-keto-L-gulonic acid which is an important intermediate in the production of vitamin C.Due to the release duties in foreign countries, the original deposits with respect to the following strains have been transferred to the deposit under the Budapest Treaty at Agency of Industrial Science and Technology, Fermentation Research Institute, Japan on Jan. 29, 1987. And the following accession numbers have been allotted to each strain.______________________________________ strain Old No. New No. ______________________________________ Gluconobacter oxydans FERM-P No. 8422, Accession No. UV-10 FERM BP-1267 Gluconobacter oxydans E-1 FERM-P No. 8353, Accession No. FERM BP-1265 Gluconobacter oxydans H-2 FERM-P No. 8354, Accession No. FERM BP-1266 Gluconobacter oxydans L-8 FERM-P No.Type: GrantFiled: May 29, 1987Date of Patent: April 10, 1990Assignee: Hoffmann-La Roche Inc.Inventors: Akiko Fujiwara, Tatsuo Hoshino, Teruhide Sugisawa
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Patent number: 4902617Abstract: The present invention relates to novel enzyme having L-sorbosone dehydrogenase activity and a process for producing the same. This L-Sorbosone dehydrogenase enzyme provided by the present invention catalyzes the oxidation of L-sorbosone to 2-keto-L-gulonic acid in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a coenzyme. 2-KGA is an important precursor for the production of vitamin C.Type: GrantFiled: May 29, 1987Date of Patent: February 20, 1990Assignee: Hoffmann-La Roche Inc.Inventors: Akiko Fujiwara, Tatsuo Hoshino, Teruhide Sugisawa