Patents by Inventor Tetsushi Sakuma
Tetsushi Sakuma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230340455Abstract: A method for editing a target DNA comprising: a step of bringing (1) a fusion protein containing a TALE and a nucleic acid base converting enzyme and (2) a CRISPR-Cas9 system containing a Cas9 protein which has lost part or all of a nuclease activity and a guide RNA thereof into contact with a target DNA to edit a base in a target site of the target DNA by using a nucleic acid base converting enzyme activity of the fusion protein, wherein a TALE recognition sequence recognized by the TALE in the fusion protein or a complementary sequence thereof is present on a 5? side of the target site via a spacer 1 having a chain length of 7 to 31 bp, and a guide RNA-target sequence recognized by the guide RNA in the CRISPR-Cas9 system is present to contain a complementary base of the target site.Type: ApplicationFiled: September 3, 2021Publication date: October 26, 2023Applicants: Hiroshima University, SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Tetsushi SAKUMA, Takashi YAMAMOTO, Nahoko NISHIBORI, Tadahiko YOSHIMA, Kazuhiko HORIGOME
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Patent number: 11788076Abstract: The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.Type: GrantFiled: October 9, 2018Date of Patent: October 17, 2023Assignees: Hiroshima University, Repertoire Genesis IncorporationInventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Takahiko Miyama, Ryuji Suzuki
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Patent number: 11773152Abstract: The present disclosure provides a technique for producing regulatory T cells specific to a desired antigen. Regulatory T cells specific to an antigen are produced by a method that includes: a step for identifying a T cell receptor (TCR) clone present in an effector T cell population specific to an antigen in an effector T cell donor; and a step for introducing all or part of the nucleic acid sequence of the TCR? gene and all or part of the nucleic acid sequence of the TCR? gene included in the clone into a regulatory T cell, said step comprising introducing the TCR? and the TCR? so as to be expressed in pairs.Type: GrantFiled: October 9, 2018Date of Patent: October 3, 2023Assignees: Hiroshima University, Reperptoire Genesis IncorprationInventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Takahiko Miyama, Ryuji Suzuki
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Publication number: 20230250415Abstract: The present disclosure provides a technique for introducing an antigen receptor-encoding nucleic acid into a definite site in a nucleic acid that is contained in an immune cell of a target organism. In the technique according to the present disclosure, a vector, which contains an antigen receptor-encoding nucleic acid, and a nuclease are introduced into an immune cell. The nuclease cleaves the vector and the genome sequence of the immune cell. The vector can be configured so that, when cleaved with the nuclease, a sequence, which is an antigen receptor-encoding sequence and which is introduced by microhomology-mediated end-joining (MMEJ) at the nuclease cleavage site of the genome sequence of the immune cell, is formed.Type: ApplicationFiled: November 13, 2020Publication date: August 10, 2023Inventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Masatoshi Nishizawa, Ryuji Suzuki, Kenta Magoori, Hiroyuki Sato
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Publication number: 20230027317Abstract: Provided is an animal model that can replicate a disease state of human AD. A non-human primate model animal of Alzheimer's disease includes the PSEN1 gene in which a site related to splicing of exon 9 is made deficient.Type: ApplicationFiled: December 18, 2020Publication date: January 26, 2023Inventors: Erika SASAKI, Kenya SATO, Wakako KUMITA, Hiroki SASAGURI, Takaomi SAIDO, Kenichi NAGATA, Takashi YAMAMOTO, Tetsushi SAKUMA
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Patent number: 11541788Abstract: A seat suspension includes a base including a base hook and a seat platform provided with a seat. A first link couples a first rotational axis of the base and a third rotational axis of the seat platform and is configured to be rotatable about the first rotational axis and the third rotational axis. A second link couples a second rotational axis of the base and a fourth rotational axis of the seat platform and is configured to be rotatable about the second rotational axis and the fourth rotational axis. A link hook is provided on the second link. An elastic member is latched to the base hook and the link hook and is configured to pull the link hook toward the base hook. A first angle between a contracting direction of the elastic member and a rotation direction of the link hook increases as the elastic member expands.Type: GrantFiled: September 9, 2019Date of Patent: January 3, 2023Assignee: KUBOTA CORPORATIONInventors: Tetsushi Sakuma, Daisuke Yasunobe, Takateru Nakagawa
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Publication number: 20220389396Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.Type: ApplicationFiled: May 13, 2022Publication date: December 8, 2022Applicant: Hiroshima UniversityInventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
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Publication number: 20220356207Abstract: An object of the present invention is to provide a cell penetrating peptide having a penetrating ability into cells. The present inventors provided a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 1, a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 2 and a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 3; and a complex comprising any one of the cell penetrating peptide and a functional molecule.Type: ApplicationFiled: June 30, 2022Publication date: November 10, 2022Applicants: Astellas Pharma Inc., National Center for Global Health and Medicine, Hiroshima UniversityInventors: Hideki ENDO, Yukihito ISHIZAKA, Akira ISHIGURO, Tomoki TAKASHINA, Takashi YAMAMOTO, Tetsushi SAKUMA
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Patent number: 11414456Abstract: The present application provides a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 1, a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 2 and a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 3; and a complex comprising any one of the cell penetrating peptide and a functional molecule.Type: GrantFiled: December 10, 2018Date of Patent: August 16, 2022Assignees: Astellas Pharma Inc., National Center for Global Health and Medicine, Hiroshima UniversityInventors: Hideki Endo, Yukihito Ishizaka, Akira Ishiguro, Tomoki Takashina, Takashi Yamamoto, Tetsushi Sakuma
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Patent number: 11390652Abstract: It is intended to provide a fusion polypeptide that regulates the transcription of a target gene. The present inventors have provided a fusion polypeptide comprising: a cell-penetrating peptide; a DNA-binding polypeptide; and a transcriptional regulator.Type: GrantFiled: December 11, 2017Date of Patent: July 19, 2022Assignees: Astellas Pharma Inc., National Center for Global Health and Medicine, Hiroshima UniversityInventors: Hideki Endo, Yukihito Ishizaka, Takashi Yamamoto, Tetsushi Sakuma
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Patent number: 11359186Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.Type: GrantFiled: June 6, 2018Date of Patent: June 14, 2022Assignee: Hiroshima UniversityInventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
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Patent number: 11279941Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.Type: GrantFiled: January 19, 2017Date of Patent: March 22, 2022Assignee: RIKENInventors: Keiji Numata, Yoko Horii, Takeshi Yoshizumi, Taku Demura, Yutaka Kodama, Takashi Yamamoto, Tetsushi Sakuma, Kenji Miura, Hiroshi Ezura
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Publication number: 20220064229Abstract: A method for designing a protein capable of binding in a DNA base selective manner or DNA base sequence specific manner is provided. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A. contained in L of the formula 1, the aforementioned object could be achieved.Type: ApplicationFiled: July 1, 2021Publication date: March 3, 2022Applicants: KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION, HIROSHIMA UNIVERSITYInventors: Takashi Yamamoto, Tetsushi Sakuma, Takahiro Nakamura, Yusuke Yagi, Yasuyuki Okawa
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Publication number: 20220064253Abstract: The present disclosure provides a technique for producing regulatory T cells specific to a desired antigen. Regulatory T cells specific to an antigen are produced by a method that includes: a step for identifying a T cell receptor (TCR) clone present in an effector T cell population specific to an antigen in an effector T cell donor; and a step for introducing all or part of the nucleic acid sequence of the TCR? gene and all or part of the nucleic acid sequence of the TCR? gene included in the clone into a regulatory T cell, said step comprising introducing the TCR? and the TCR? so as to be expressed in pairs.Type: ApplicationFiled: October 9, 2018Publication date: March 3, 2022Inventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Takahiko Miyama, Ryuji Suzuki
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Publication number: 20210332339Abstract: The present invention provides an artificial nucleic acid-cleaving enzyme comprising: a nuclease domain which is a polypeptide containing an amino acid sequence set forth at positions 391 to 585 of SEQ ID NO: 1 or positions 389 to 579 of SEQ ID NO: 3, or a mutant polypeptide thereof; and a nucleic acid-binding domain.Type: ApplicationFiled: August 23, 2019Publication date: October 28, 2021Applicant: HIROSHIMA UNIVERSITYInventors: Takashi YAMAMOTO, Tetsushi SAKUMA, Masakazu SAITO
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Publication number: 20210324019Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A.Type: ApplicationFiled: July 1, 2021Publication date: October 21, 2021Applicants: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, HIROSHIMA UNIVERSITYInventors: Takashi Yamamoto, Tetsushi Sakuma, Takahiro Nakamura, Yusuke Yagi, Yasuyuki Okawa
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Publication number: 20210163533Abstract: An object of the present invention is to provide a cell penetrating peptide having a penetrating ability into cells. The present inventors provided a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 1, a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 2 and a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 3; and a complex comprising any one of the cell penetrating peptide and a functional molecule.Type: ApplicationFiled: December 10, 2018Publication date: June 3, 2021Applicants: Astellas Pharma Inc., National Center for Global Health and Medicine, Hiroshima UniversityInventors: Hideki Endo, Yukihito ISHIZAKA, Akira ISHIGURO, Tomoki TAKASHINA, Takashi YAMAMOTO, Tetsushi SAKUMA
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Patent number: 10907589Abstract: A work vehicle includes an engine, a particulate filter, and an oil separator. The particulate filter is connected to the engine to collect particulate matters contained in exhaust gas from the engine. The particulate filter is provided to overlap with the engine when viewed in a height direction along a height of the work vehicle. The oil separator is connected to the engine via a blow-by-gas discharge pipe to catch a liquid component in blow-by-gas from the engine. The oil separator is provided between the engine and the particulate filter to overlap with the particulate filter when viewed in the height direction.Type: GrantFiled: November 25, 2019Date of Patent: February 2, 2021Assignee: KUBOTA CORPORATIONInventors: Tetsushi Sakuma, Akiyoshi Ono, Megumi Sawai, Daisuke Inaba, Shinji Kato
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Publication number: 20200362323Abstract: The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.Type: ApplicationFiled: October 9, 2018Publication date: November 19, 2020Inventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Takahiko Miyama, Ryuji Suzuki
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Patent number: D1017884Type: GrantFiled: April 22, 2021Date of Patent: March 12, 2024Assignee: KUBOTA CORPORATIONInventors: Tetsushi Sakuma, Megumi Sawai, Taiki Furuki, Yuki Tomida