Patents by Inventor Thierry Bettinger
Thierry Bettinger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11905323Abstract: The invention discloses a recombinant protein (P-selectin glycoprotein ligand-1 and Neural Retina-specific Leucine Zipper) PSGL-1-NRL chimeric protein comprising a Selectin Binding domain and a non-covalent dimerization domain, which is a leucine zipper and is more preferably the leucine zipper domain of the human or mouse Neural Retina-specific Leucine Zipper. The chimeric protein further comprises a covalent dimerization domain with at least one cysteine suitable to form a disulfide bridge with another chimeric protein to form a homodimer. In the chimeric protein, the PSGL-1 domain corresponds to the extracellular region of Human PSGL-1 and is more preferably the selectin binding region of the mature protein. The chimeric protein is correctly post-translationally modified and is efficiently expressed in a mammalian system. It is sulfated, O-linked glycosylated and sialylated and binds P, E and L selectin, allowing in vivo and in vitro targeting for diagnostic or therapeutic purposes.Type: GrantFiled: May 18, 2022Date of Patent: February 20, 2024Assignee: Bracco SuisseInventors: Federico Maisano, Federico Crivellin, Thierry Bettinger, Philippe Bussat, Samir Cherkaoui, Christian Koller, Adrian Lobito
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Publication number: 20220389080Abstract: The invention discloses a recombinant protein (P-selectin glycoprotein ligand-1 and Neural Retina-specific Leucine Zipper) PSGL-1-NRL chimeric protein comprising a Selectin Binding domain and a non-covalent dimerization domain, which is a leucine zipper and is more preferably the leucine zipper domain of the human or mouse Neural Retina-specific Leucine Zipper. The chimeric protein further comprises a covalent dimerization domain with at least one cysteine suitable to form a disulfide bridge with another chimeric protein to form a homodimer. In the chimeric protein, the PSGL-1 domain corresponds to the extracellular region of Human PSGL-1 and is more preferably the selectin binding region of the mature protein. The chimeric protein is correctly post-translationally modified and is efficiently expressed in a mammalian system. It is sulfated, O-linked glycosylated and sialylated and binds P, E and L selectin, allowing in vivo and in vitro targeting for diagnostic or therapeutic purposes.Type: ApplicationFiled: May 18, 2022Publication date: December 8, 2022Applicant: Bracco Suisse SAInventors: Federico MAISANO, Federico CRIVELLIN, Thierry BETTINGER, Philippe BUSSAT, Samir CHERKAOUI, Christian KOLLER, Adrian LOBITO
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Patent number: 11370826Abstract: The invention discloses a recombinant protein (P-selectin glycoprotein ligand-1 and Neural Retina-specific Leucine Zipper) PSGL-1-NRL chimeric protein comprising a Selectin Binding domain and a non-covalent dimerization domain, which is a leucine zipper and is more preferably the leucine zipper domain of the human or mouse Neural Retina-specific Leucine Zipper. The chimeric protein further comprises a covalent dimerization domain with at least one cysteine suitable to form a disulfide bridge with another chimeric protein to form a homodimer. In the chimeric protein, the PSGL-1 domain corresponds to the extracellular region of Human PSGL-1 and is more preferably the selectin binding region of the mature protein. The chimeric protein is correctly post-translationally modified and is efficiently expressed in a mammalian system. It is sulfated, O-linked glycosylated and sialylated and binds P, E and L selectin, allowing in vivo and in vitro targeting for diagnostic or therapeutic purposes.Type: GrantFiled: February 9, 2017Date of Patent: June 28, 2022Assignee: Bracco Suisse SAInventors: Federico Maisano, Federico Crivellin, Thierry Bettinger, Philippe Bussat, Samir Cherkaoui, Christian Koller, Adrian Lobito
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Publication number: 20190048062Abstract: The invention discloses a recombinant protein (P-selectin glycoprotein ligand-1 and Neural Retina-specific Leucine Zipper) PSGL-1-NRL chimeric protein comprising a Selectin Binding domain and a non-covalent dimerization domain, which is a leucine zipper and is more preferably the leucine zipper domain of the human or mouse Neural Retina-specific Leucine Zipper. The chimeric protein further comprises a covalent dimerization domain with at least one cysteine suitable to form a disulfide bridge with another chimeric protein to form a homodimer. In the chimeric protein, the PSGL-1 domain corresponds to the extracellular region of Human PSGL-1 and is more preferably the selectin binding region of the mature protein. The chimeric protein is correctly post-translationally modified and is efficiently expressed in a mammalian system. It is sulfated, O-linked glycosylated and sialylated and binds P, E and L selectin, allowing in vivo and in vitro targeting for diagnostic or therapeutic purposes.Type: ApplicationFiled: February 9, 2017Publication date: February 14, 2019Applicant: Bracco Suisse SAInventors: Federico MAISANO, Federico CRIVELLIN, Thierry BETTINGER, Philippe BUSSAT, Samir CHERKAOUI, Christian KOLLER, Adrian LOBITO
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Patent number: 10010630Abstract: A formulation for preparing gas-filled microvesicles which comprises a phospholipid and a mixture of saturated and unsaturated fatty acids. The gas-filled microvesicles having a stabilizing layer comprising said composition show an increased stability with respect to microvesicles containing only a saturated or an unsaturated fatty acid.Type: GrantFiled: December 19, 2013Date of Patent: July 3, 2018Assignee: BRACCO SUISSE S.A.Inventors: Anne Lassus, Philippe Bussat, Thierry Bettinger
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Patent number: 9375397Abstract: Composition comprising gas filled microcapsules and a bioactive agent, useful for an ultrasound-mediated delivery of said bioactive agent. The microcapsules comprise a relatively stiff shell of polymeric or lipid material and have in particular a resistance to a mechanical index of at least 0.15, while the bioactive agent is substantially unbound to the shell of the microcapsules. The composition of the invention is particularly suitable for effectively delivering a genetic material into a cell, upon exposure of the composition to a level of acoustic pressure capable of destroying a portion of the microcapsules and releasing the gas contained therein.Type: GrantFiled: April 28, 2011Date of Patent: June 28, 2016Assignee: BRACCO SUISSE S.A.Inventors: Thierry Bettinger, Feng Yan, Sophie Mehier-Humbert, Peter Frinking
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Patent number: 9333273Abstract: Gas-filled microvesicles associated with a polypeptide comprising a sequence of amino acids, said sequence exhibiting binding affinity for selectins, particularly p-selectin. The gas-filled microvesicles can be used in ultrasound imaging.Type: GrantFiled: October 30, 2015Date of Patent: May 10, 2016Assignee: BRACCO SUISSE S.A.Inventors: Thierry Bettinger, Philippe Bussat, Samir Cherkaoui, Irene Guilbert-Brigger, Bernard Lamy
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Publication number: 20160074539Abstract: Gas-filled microvesicles associated with a polypeptide comprising a sequence of amino acids, said sequence exhibiting binding affinity for selectins, particularly p-selectin. The gas-filled microvesicles can be used in ultrasound imaging.Type: ApplicationFiled: October 30, 2015Publication date: March 17, 2016Applicant: BRACCO SUISSE S.A.Inventors: Thierry BETTINGER, Philippe BUSSAT, Samir CHERKAOUI, Irene GUILBERT-BRIGGER, Bernard LAMY
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Patent number: 9211348Abstract: Gas-filled microvesicles associated with a polypeptide comprising a sequence of amino acids, said sequence exhibiting binding affinity for selectins, particularly p-selectin. The gas-filled microvesicles can be used in ultrasound imaging.Type: GrantFiled: August 9, 2011Date of Patent: December 15, 2015Assignee: BRACCO SUISSE S.A.Inventors: Thierry Bettinger, Philippe Bussat, Samir Cherkaoui, Irene Guilbert-Brigger, Bernard Lamy
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Publication number: 20150343097Abstract: A formulation for preparing gas-filled microvesicles which comprises a phospholipid and a mixture of saturated and unsaturated fatty acids. The gas-filled microvesicles having a stabilizing layer comprising said composition show an increased stability with respect to microvesicles containing only a saturated or an unsaturated fatty acid.Type: ApplicationFiled: December 19, 2013Publication date: December 3, 2015Applicant: BRACCO SUISSE S.A.Inventors: Anne LASSUS, Philippe BUSSAT, Thierry BETTINGER
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Patent number: 9192685Abstract: Pharmaceutical kit comprising (i) a targeting construct which comprises a targeting ligand and an anti-polymer antibody and (ii) a polymer-containing liposome or gas-filled microvesicle capable of binding or associating to said construct. Also disclosed are methods of preparing and of administering said assembly, as well as an assembly comprising said targeting construct and said liposome or gas-filled microvesicle.Type: GrantFiled: October 7, 2009Date of Patent: November 24, 2015Assignee: Bracco Suisse S.A.Inventors: Eric Allemann, Thierry Bettinger, Philippe Bussat, Mathieu Hauwel, Feng Yan
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Publication number: 20130156706Abstract: Gas-filled microvesicles associated with a polypeptide comprising a sequence of amino acids, said sequence exhibiting binding affinity for selectins, particularly p-selectin. The gas-filled microvesicles can be used in ultrasound imaging.Type: ApplicationFiled: August 9, 2011Publication date: June 20, 2013Applicant: BRACCO SUISSE S.A.Inventors: Thierry Bettinger, Philippe Bussat, Samir Cherkaoui, Irene Guilbert-Brigger, Bernard Lamy
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Publication number: 20120093731Abstract: Gas-filled microvesicles comprising a boundary envelope containing a gas, wherein said microvesicles comprise: —a first component, bound to said envelope, having binding affinity for an Fc-region of an antibody; and—a second component comprising a Fc-region of an antibody, bound to said first component through said Fc-region, said second component comprising a targeting ligand or a therapeutic agent. Aqueous suspensions of said microvesicles are particularly useful in contrast enhanced ultrasound imaging.Type: ApplicationFiled: December 19, 2007Publication date: April 19, 2012Applicant: Bracco Research S.A.Inventors: Eric Allemann, Thierry Bettinger, Philippe Bussat
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Publication number: 20110208115Abstract: Composition comprising gas filled microcapsules and a bioactive agent, useful for an ultrasound-mediated delivery of said bioactive agent. The microcapsules comprise a relatively stiff shell of polymeric or lipid material and have in particular a resistance to a mechanical index of at least 0.15, while the bioactive agent is substantially unbound to the shell of the microcapsules. The composition of the invention is particularly suitable for effectively delivering a genetic material into a cell, upon exposure of the composition to a level of acoustic pressure capable of destroying a portion of the microcapsules and releasing the gas contained therein.Type: ApplicationFiled: April 28, 2011Publication date: August 25, 2011Applicant: BRACCO RESEARCH S.A.Inventors: Thierry BETTINGER, Feng YAN, Sophie MEHIER-HUMBERT, Peter FRINKING
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Publication number: 20110200530Abstract: Pharmaceutical kit comprising (i) a targeting construct which comprises a targeting ligand and an anti-polymer antibody and (ii) a polymer-containing liposome or gas-filled microvesicle capable of binding or associating to said construct. Also disclosed are methods of preparing and of administering said assembly, as well as an assembly comprising said targeting construct and said liposome or gas-filled microvesicle.Type: ApplicationFiled: October 7, 2009Publication date: August 18, 2011Applicant: Bracco Research S.A. or Bracco Suisse S.A.Inventors: Eric Allemann, Thierry Bettinger, Philippe Bussat, Mathieu Hauwel, Feng Yan
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Publication number: 20100086490Abstract: Gas-filled microvesicles comprising a boundary envelope containing a gas, wherein said microvesicles comprise: —a first component, bound to said envelope, having binding affinity for an Fc-region of an antibody; and—a second component comprising a Fc-region of an antibody, bound to said first component through said Fc-region, said second component comprising a targeting ligand or a therapeutic agent. Aqueous suspensions of said microvesicles are particularly useful in contrast enhanced ultrasound imaging.Type: ApplicationFiled: December 19, 2007Publication date: April 8, 2010Applicant: Bracco Research S.A.Inventors: Eric Allemann, Thierry Bettinger, Philippe Bussat
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Publication number: 20090041833Abstract: Composition comprising gas filled microcapsules and a bioactive agent, useful for an ultrasound-mediated delivery of said bioactive agent. The microcapsules comprise a relatively stiff shell of polymeric or lipid material and have in particular a resistance to a mechanical index of at least 0.15, while the bioactive agent is substantially unbound to the shell of the microcapsules. The composition of the invention is particularly suitable for effectively delivering a genetic material into a cell, upon exposure of the composition to a level of acoustic pressure capable of destroying a portion of the microcapsules and releasing the gas contained therein.Type: ApplicationFiled: April 11, 2006Publication date: February 12, 2009Applicant: Bracco Research S.A.Inventors: Thierry Bettinger, Feng Yan, Sophie Mehier-Humbert, Peter Frinking
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Publication number: 20070128117Abstract: Method for preparing a lyophilized matrix and, upon reconstitution of the same, a respective injectable contrast agent comprising a liquid aqueous suspension of gas-filled microbubbles stabilized predominantly by a phospholipid and comprising a ligand agent. The method comprises preparing an emulsion from an aqueous medium, comprising a phospholipid and a water immiscible organic solvent. A suspension of a compound comprising the ligand agent or a precursor thereof is then added to emulsion. The emulsion is then freeze-dried and subsequently reconstituted in an aqueous suspension of gas-filled microbubbles.Type: ApplicationFiled: December 19, 2006Publication date: June 7, 2007Applicant: Bracco International B.V.Inventors: Thierry Bettinger, Philippe Bussat