Abstract: The technology relates in part to biological methods for producing terpenes and to engineered cells and microorganisms capable of such production.

Abstract: The invention provides engineered biosynthetic pathways that can be used to produce cannabinoids from fatty acids, recombinant microorganisms incorporating such pathways, methods of biosynthetically producing cannabinoids from fatty acids, and cannabinoids so produced.

Abstract: The technology relates in part to biological methods for producing terpenes and to engineered cells and microorganisms capable of such production.

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract: Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees C. and at least one year at ?20 degrees C. Methods of using the markers and kits containing stable native protein markers in liquid form for determining molecular mass of proteins using electrophoresis are also provided. Furthermore, methods for generating revenue by selling the liquid molecular weight markers, are provided.

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract: Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees C. and at least one year at ?20 degrees C. Methods of using the markers and kits containing stable native protein markers in liquid form for determining molecular mass of proteins using electrophoresis are also provided. Furthermore, methods for generating revenue by selling the liquid molecular weight markers, are provided.

Abstract: Disclosed are methods for the characterization of allergens using recombinant fusion protein in an enzyme-linked immunosorbent (ELISA) assay. Also disclosed are methods for determining allergen sensitivity and methods for immunotherapy using the ELISA of the present invention.