Abstract: The present invention provides a method for clone-multiplication of a Paphiopedilum wherein a scape terminal bud or an axillary shoot, whose surface has been sterilized, or an aseptic shoot obtained by an aseptic cultivation, of a Paphiopedilum stock, are cut into cross-sectional pieces which are then cultivated in a medium containing a plant hormone. By cutting an aseptic shoot containing a number of axillary buds into cross-sectional pieces and cultivating, a multishoot formation is feasible. Also by employing a liquid medium containing a solid support such as a rockwool or a paper filter in the culture, the plant death due to browning of tissues can effectively be prevented. In addition, by optimizing the method for sterilizing the buds before blooming, the medium composition and the culture condition, a large scale production of Paphiopedilums by means of a clone multiplication technology becomes feasible.

Abstract: Disclosed is a method for producing Phalaenopsis orchid clone plants, which comprises cutting the tip of a growing root of a Phalaenopsis orchid that has been prepared under sterile conditions at a length of from 1 to 5 mm to prepare a root tip for culture having said predetermined length, cultivating said root tip in a PLB-inducing medium under sterile conditions to thereby induce PLB therein, further cultivating said PLB in a propagating medium, and then re-differentiating said PLB in a re-differentiation medium. The method is practicable and stably given PLB of Phalaenopsis orchids.