Abstract: A liquid yeast one- and two-hybrid high-throughput library screening method and use thereof changes the screening of solid medium to liquid culture solution screening, avoiding a large number of plate coating work, and avoiding the tedious work of identifying single colonies one by one, which not only saves experiment time, but also reduces the cost and workload; at the same time, the present invention also combines high-throughput sequencing technology to improve the analysis throughput of yeast hybridization screening work, and can also improve the automation level of analysis, and can greatly reduce false negatives. More importantly, the present invention can solve the phenomenon of protein self-activation. By changing to liquid screening, known protein interaction information can be successfully obtained, which provides a new opportunity for protein-protein interaction research.

Abstract: A system and method of converting waste plastics into hydrocarbon oil comprises a thermal cracking reactor, into which the waste plastics are cracked at a temperature in the range of 270-800° C. to obtain partly gaseous hydrocarbons, partly liquid hydrocarbons, and remaining residues. A continuous thermal cracking and residual discharging portion is connected to have the liquid hydrocarbons gradually and fully cracked into gaseous hydrocarbons, while the residues are discharged at a residual discharge outlet. A chlorine removal portion is connected to receive the gaseous hydrocarbons to remove chlorine from it. A catalytic cracking reactor is connected to the chlorine removal portion to have the gaseous hydrocarbons catalytic cracking with an acid catalyst. A three-stage cooling portion is adopted to have the catalytically cracked gaseous hydrocarbons fully converted into liquid hydrocarbons, i.e., hydrocarbon oil. A pressurized activation reaction portion is provided to remove few amount of S. N. P.