Abstract: The present invention relates to a polymer microsphere comprising at least one polymer and at least one bound latent colorant, wherein the microsphere is stabilized by at least one stabilizing polymer. The invention also includes a method of preparing polymer microspheres comprising combining latent colorant, ethylenically unsaturated monomer, stabilizing polymer, and an initiator in solvent and initiating polymerization to form a polymeric microsphere stabilized by a stabilizing polymer bound to the surface of the polymeric microsphere.

Abstract: A protein microarray element comprising: a) a support; b) a gelatin layer containing functional groups capable of binding biological probes; and interposed between the support and the gelatin layer and c) an adhesive interlayer layer capable of maintaining contact with the support and with the gelatin layer.

Abstract: A microarray comprising: a support, on which is disposed a layer of microspheres bearing biological probes; wherein said microspheres comprise at least one material with a latent color that can be developed and used to identify said microsphere. A method of identifying biological analytes using the microarray is also disclosed.

Abstract: A method of preparing polymeric particles with photographic couplers, such polymeric particles being useful in a microarray, the method comprising the steps of: (a) preparing an organic composition by combining a photographic coupler and a high boiling organic solvent; (b) preparing a separate aqueous composition comprising polymeric particles with functionally active groups on their surfaces; (c) combining the organic composition of step (a) with the aqueous composition of step (b), thereby effecting a loading of said photographic coupler and high boiling organic solvent into said polymeric particles; and (d) attaching biological probes to the functionally active groups on the surfaces of the loaded polymeric particles of step c). Polymeric particles made by the method is also disclosed.

Abstract: The present invention relates to a polymer microsphere comprising at least one polymer and at least one bound latent colorant, wherein the microsphere is stabilized by at least one stabilizing polymer. The invention also includes a method of preparing polymer microspheres comprising combining latent colorant, ethylenically unsaturated monomer, stabilizing polymer, and an initiator in solvent and initiating polymerization to form a polymeric microsphere stabilized by a stabilizing polymer bound to the surface of the polymeric microsphere.

Abstract: A gelatin-based substrate for fabricating protein arrays, the substrate comprising: gelatin having at least one surface; a polymer scaffold affixed to the gelatin surface; wherein the polymer in the scaffold is rich in reactive units capable of immobilizing proteins.

Abstract: A gelatin-based substrate for fabricating protein arrays, the substrate containing: gelatin and a trifunctional compound A—L—B; wherein A is a functional group capable of interacting with the gelatin; L is a linking group capable of interacting with A and with B; and B is a functional group capable of interacting with a protein capture agent. A may be the same or different from B.

Abstract: A method of making a microarray comprising the steps of: providing a support; coating on the support a fluid composition containing microspheres and gelatin; immobilizing the microspheres in the gelatin coating; partially digesting the gelatin with an enzyme to expose surfaces of the microspheres; and removing the enzyme and digested gelatin from the coating.

Abstract: In accordance with one embodiment of the invention, a photographic element is described comprising a support bearing one or more hydrophilic colloid layers including at least one photographic silver halide emulsion layer, wherein sequestered silver ions are incorporated into at least one hydrophilic colloid layer in the form of a silver ion containing material which sequesters silver ions prior to photographic processing and releases silver ions upon exposure to photographic processing solutions. Method for preparing and processing such photographic element are also described. In accordance with particular embodiments of the invention, the silver ion containing material comprises a silver ion-exchanged zeolite material or an intercalation composition comprising a layered host material having silver ions inserted as guest ions between the layers of the host material.

Abstract: A method of identifying nucleic acid samples comprising: providing a mircoarray including a substrate coated with a composition including a population of nucleic acid probe modified micro-spheres immobilized in a coating containing a gelling agent or a precursor to a gelling agent, wherein a first portion of the micro-spheres is submerged in the gelatin coating and a second portion is exposed above the gelatin coating and is substantially free of gelatin, at least one sub-population of the population micro-spheres containing an optical barcode generated from at least one colorant associated with the micro-spheres and including a nucleic acid probe sequence; contacting the array with a target nucleic acid sequence; and detecting the color barcode of the sub-population of micro-spheres due to the interaction of the probe nucleic acid sequence and the fluorescently/chemiluminescently labeled nucleic acid sample target nucleic acid sequence.

Abstract: A gelatin-based substrate for fabricating protein arrays, the substrate comprising:

Abstract: A method of making a microarray comprising the steps of:

Abstract: A method for single molecule identification of a target DNA molecule in a random coil state having the following steps: a) attaching an optically distinguishable material to a DNA sequence recognition unit; b) hybridizing the DNA sequence recognition unit to the target DNA molecule in a random coil state to form a hybridized DNA complex in a random coil state; c) passing the hybridized DNA complex in a random coil state from a reservoir in a microfluidic device through a narrow channel to cause an acceleration of flow through the channel, thereby causing the hybridized DNA complex to extend into a substantially linear configuration; and d) detecting the optically distinguishable material in a sequential manner along the substantially linear hybridized DNA complex, thereby identifying the target DNA molecule.

Abstract: In accordance with one embodiment of the invention, a photographic element is described comprising a support bearing one or more hydrophilic colloid layers including at least one photographic silver halide emulsion layer, wherein sequestered silver ions are incorporated into at least one hydrophilic colloid layer in the form of a silver ion containing material which sequesters silver ions prior to photographic processing and releases silver ions upon exposure to photographic processing solutions. Method for preparing and processing such photographic element are also described. In accordance with particular embodiments of the invention, the silver ion containing material comprises a silver ion-exchanged zeolite material or an intercalation composition comprising a layered host material having silver ions inserted as guest ions between the layers of the host material.

Abstract: A method of identifying nucleic acid samples comprising:

Abstract: A method for single molecule identification of a target DNA molecule in a random coil state having the following steps: a) attaching an optically distinguishable material to a DNA sequence recognition unit; b) hybridizing the DNA sequence recognition unit to the target DNA molecule in a random coil state to form a hybridized DNA complex in a random coil state; c) stretching the hybridized DNA complex in a random coil state to form a hybridized DNA complex in a substantially linear configuration; and d) detecting the optically distinguishable material in a sequential manner along the substantially linear hybridized DNA complex, thereby identifying the target DNA molecule.

Abstract: A gelatin-based substrate for fabricating protein arrays, the substrate containing: gelatin and a trifunctional compound A-L-B; wherein A is a functional group capable of interacting with the gelatin; L is a linking group capable of interacting with A and with B; and B is a functional group capable of interacting with a protein capture agent. A may be the same or different from B.

Abstract: A coating composition for making a microarray comprising: a gelling agent or a precursor to a gelling agent and microspheres dispersed in a fluid; wherein, upon coating the composition on a substrate, said microspheres become immobilized in the plane of coating and form a random pattern on the substrate. The substrate is characterized by an absence of specific sites capable of interacting physically or chemically with the microspheres.

Abstract: The present invention is a photographic element which includes a support, at least one silver halide emulsion layer superposed on the support and a processing-solution-permeable protective overcoat overlying the silver halide emulsion layer that becomes water-resistant in the final product without lamination or fusing. The present invention is also directed to a method of making a photographic print involving developing the photographic element.

Abstract: This invention relates to a silver halide photographic element comprising a support and at least one gelatin containing imaging or non-imaging layer wherein during chemical processing the maximum swell of the layer is greater than the equilibrium swell.