Patents by Inventor Tiiu Mandel

Tiiu Mandel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Viral expression plasmids for production of proteins, antibodies, enzymes, virus-like particles and for use in cell-based assays
    Publication number: 20130236958
    Abstract: This disclosure shows that the EBV FR-element comprised of EBNA1 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT).
    Type: Application
    Filed: January 23, 2013
    Publication date: September 12, 2013
    Applicant: ICOSAGEN CELL FACTORY OU
    Inventors: Toomas SILLA, Ingrid TAGEN, Jelizaveta GEIMANEN, Kadri JANIKSON, Aare ABROI, Ene USTAV, Mart USTAV, Tiiu MANDEL, Urve TOOTS, Andres TOVER, Anne KALLING, Radi TEGOVA
  • Viral expression plasmids for production of proteins, antibodies, enzymes, virus-like particles and for use in cell-based assays
    Patent number: 8377653
    Abstract: This disclosure shows that the EBV FR-element comprised of EBNA1 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT).
    Type: Grant
    Filed: July 7, 2010
    Date of Patent: February 19, 2013
    Assignee: Icosagen Cell Factory Oü
    Inventors: Toomas Silla, Ingrid Tagen, Anne Kalling, Radi Tegova, Mart Ustav, Tiiu Mandel, Urve Toots, Andres Tover, Aare Abroi, Ene Ustav, Jelizaveta Geimanen, Kadri Janikson
  • Viral expression plasmids for production of proteins, antibodies, enzymes, virus-like particles and for use in cell-based assays
    Publication number: 20110076760
    Abstract: This disclosure shows that the EBV FR-element comprised of EBNA1 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT).
    Type: Application
    Filed: July 7, 2010
    Publication date: March 31, 2011
    Applicant: Icosagen S.A.
    Inventors: Toomas Silla, Ingrid Tagen, Anne Kalling, Radi Tegova, Mart Ustav, Tiiu Mandel, Urve Toots, Andres Tover, Aare Abroi, Ene Ustav, Jelizaveta Geimanen, Kadri Janikson
  • Vectors, cell lines and their use in obtaining extended episomal maintenance replication of hybrid plasmids and expression of gene products
    Patent number: 7790446
    Abstract: This disclosure shows that the BPV-1 E2 protein-dependent minichromosome maintenance element (MME) comprised of E2 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). MME dependent plasmids are lost with the frequency of 6% per generation. Significantly long stable maintenance replication can also be provided without selection pressure. We also demonstrate that PyV core origin maintenance function/replication activation could be provided by Epstein-Barr virus Family of repeats and EBNA1 protein. The maintenance of the Polyomavirus core origin plasmid was characterized by 13% loss of the plasmid during one cell generation in the case of EBV FR harboring plasmids.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: September 7, 2010
    Assignee: Kosagen Cell Factory Oü
    Inventors: Toomas Silla, Ingrid Tagen, Jelizaveta Geimanen, Kadri Janikson, Aare Abroi, Ene Ustav, Mart Ustav, Tiiu Mandel
  • Vectors, cell lines and their use in obtaining extended episomal maintenance replication of hybrid plasmids and expression of gene products
    Publication number: 20060183230
    Abstract: This disclosure shows that the BPV-1 E2 protein-dependent minichromosome maintenance element (MME) comprised of E2 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). MME dependent plasmids are lost with the frequency of 6% per generation. Significantly long stable maintenance replication can also be provided without selection pressure. We also demonstrate that PyV core origin maintenance function/replication activation could be provided by Epstein-Barr virus Family of repeats and EBNA1 protein. The maintenance of the Polyomavirus core origin plasmid was characterized by 13% loss of the plasmid during one cell generation in the case of EBV FR harboring plasmids.
    Type: Application
    Filed: February 10, 2006
    Publication date: August 17, 2006
    Inventors: Toomas Silla, Ingrid Tagen, Jelizaveta Geimanen, Kadri Janikson, Aare Abroi, Ene Ustav, Mart Ustav, Tiiu Mandel