Patents by Inventor Tim Patno
Tim Patno has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120063653Abstract: An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: ApplicationFiled: August 9, 2010Publication date: March 15, 2012Applicant: Nanosphere, Inc.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Patent number: 7888107Abstract: A method and apparatus are provided for processing a nucleic acid. The apparatus includes a disposable self-contained processing module that contains the nucleic acid and substantially all of the fluids to effect a nanoparticle hybridization test, a pump coupled to the processing module, a valving system disposed between the pump and processing module and a control system coupled to the pump and valving system causing the processing fluids to interact with the nucleic acid to effect a sandwich hybridization test using nanoparticles.Type: GrantFiled: July 24, 2006Date of Patent: February 15, 2011Assignee: Nanosphere, Inc.Inventors: Tim Patno, Tom Westberg, Mark Weber, Benjamin Rush, Christopher Khoury, David Morrow, Janusz Ruszel, Christian Kronshage, William Cork
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Publication number: 20110008907Abstract: A method and apparatus are provided for processing a nucleic acid. The apparatus includes a disposable self-contained processing module that contains the nucleic acid and substantially all of the fluids to effect a nanoparticle hybridization test, a pump coupled to the processing module, a valving system disposed between the pump and processing module and a control system coupled to the pump and valving system causing the processing fluids to interact with the nucleic acid to effect a sandwich hybridization test using nanoparticles.Type: ApplicationFiled: July 24, 2006Publication date: January 13, 2011Inventors: Tim Patno, Tom Westberg, Mark Weber, Benjamin Rush, Christopher Khoury, David Morrow, Janusz Ruszel, Christian Kronshage, William Cork
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Patent number: 7773790Abstract: An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: GrantFiled: September 8, 2006Date of Patent: August 10, 2010Assignee: Nanosphere, Inc.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Patent number: 7695952Abstract: A disposable sample processing module is provided for processing DNA or RNA samples. The module includes a hybridization chamber adapted to receive an oligonucleotide covalently bonded to an internal surface of the hybridization chamber. The module also include a sample well adapted to hold a DNA or RNA sample, said sample well being coupled to the hybridization chamber, a moveable valve plate disposed between the sample well and hybridization chamber, said moveable valve plate having a first position that allows transfer of the DNA or RNA sample from the sample well to the hybridization chamber and a second position that blocks transfer to the hybridization chamber and a manifold adapted to exchange fluids with the hybridization chamber to hybridize the DNA or RNA sample with the oligonucleotide, to wash the hybridized sample and to amplify the hybridized sample.Type: GrantFiled: November 5, 2004Date of Patent: April 13, 2010Assignee: Nanosphere, Inc.Inventors: Tim Patno, Tom Westberg
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Publication number: 20100033724Abstract: An apparatus and method for imaging metallic nanoparticles. The invention teaches an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, and illumination module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provides for a compact system without the need for complex motorized devices to move a camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: ApplicationFiled: January 16, 2009Publication date: February 11, 2010Applicant: NANOSPHERE, INC.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Patent number: 7625746Abstract: A method and apparatus are provided for processing a DNA or RNA sample within a sample processing module. The method includes the steps of providing a sample well within the sample processing module that contains the DNA or RNA sample, coupling ultrasonic energy from an external source into the sampling well and denaturing and fragmenting the DNA or RNA sample using the ultrasonic energy.Type: GrantFiled: July 24, 2006Date of Patent: December 1, 2009Assignee: Nanosphere, Inc.Inventors: Tim Patno, Tom Westberg, Michael F. Halblander, Emily R. Beeson, Benjamin L. Rush
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Publication number: 20080268458Abstract: A method is provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.Type: ApplicationFiled: July 8, 2008Publication date: October 30, 2008Applicant: Nanosphere, Inc.Inventors: Tim Patno, Jennifer Hollenstein, Christian Kronshage, Christopher Khoury, Mark Weber, Tom Westberg, William Cork
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Patent number: 7396677Abstract: A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.Type: GrantFiled: November 7, 2003Date of Patent: July 8, 2008Assignee: Nanosphere, Inc.Inventors: Tim Patno, Jennifer Hollenstein, Christian Kronshage, Christopher Khoury, Mark Weber, Tom Westberg, William Cork
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Publication number: 20080020380Abstract: A method and apparatus are provided for processing a DNA or RNA sample within a sample processing module. The method includes the steps of providing a sample well within the sample processing module that contains the DNA or RNA sample, coupling ultrasonic energy from an external source into the sampling well and denaturing and fragmenting the DNA or RNA sample using the ultrasonic energy.Type: ApplicationFiled: July 24, 2006Publication date: January 24, 2008Inventors: Tim Patno, Tom Westberg, Michael F. Halblander, Emily R. Beeson, Benjamin L. Rush
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Publication number: 20070148665Abstract: An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: ApplicationFiled: September 8, 2006Publication date: June 28, 2007Applicant: Nanosphere Inc.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Publication number: 20070041624Abstract: An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: ApplicationFiled: September 8, 2006Publication date: February 22, 2007Applicant: Nanosphere Inc.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Patent number: 7110585Abstract: An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: GrantFiled: August 2, 2002Date of Patent: September 19, 2006Assignee: Nanosphere, Inc.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Publication number: 20050170493Abstract: A disposable sample processing module is provided for processing DNA or RNA samples. The module includes a hybridization chamber adapted to receive an oligonucleotide covalently bonded to an internal surface of the hybridization chamber. The module also include a sample well adapted to hold a DNA or RNA sample, said sample well being coupled to the hybridization chamber, a moveable valve plate disposed between the sample well and hybridization chamber, said moveable valve plate having a first position that allows transfer of the DNA or RNA sample from the sample well to the hybridization chamber and a second position that blocks transfer to the hybridization chamber and a manifold adapted to exchange fluids with the hybridization chamber to hybridize the DNA or RNA sample with the oligonucleotide, to wash the hybridized sample and to amplify the hybridized sample.Type: ApplicationFiled: November 5, 2004Publication date: August 4, 2005Inventors: Tim Patno, Tom Westberg
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Publication number: 20050112583Abstract: A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.Type: ApplicationFiled: November 7, 2003Publication date: May 26, 2005Inventors: Tim Patno, Jennifer Hollenstein, Christian Kronshage, Christopher Khoury, Mark Weber, Tom Westberg, William Cork
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Publication number: 20040146917Abstract: An apparatus and method for imaging metallic nanoparticles. The invention teaches an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact system without the need for complex motorized devices to move a camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: ApplicationFiled: January 15, 2004Publication date: July 29, 2004Applicant: Nanosphere, Inc.Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
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Publication number: 20030224505Abstract: An apparatus and method for DNA hybridization is provided. The apparatus and method work in conjunction with a substrate comprising an upper surface having probes. The apparatus may comprise a material which abuts the substrate, with at least a portion of the material being pliable. The material and the substrate form a plurality of chambers, each chamber having a bottom including at least a portion of the upper surface, at least one sidewall, and an opening. The apparatus further comprises a mechanism for closing the openings of the chambers, thereby sealing the chambers.Type: ApplicationFiled: January 27, 2003Publication date: December 4, 2003Inventors: Tim Patno, Mark Fisher, George Kyaw Soe Maung, Tom Westberg
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Publication number: 20030068638Abstract: An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.Type: ApplicationFiled: August 2, 2002Publication date: April 10, 2003Inventors: William Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham