Abstract: A device for forming an immersion film between a front lens of a microscope objective and a preparation on an inverted microscope. The device includes a protection device for preventing fluid leakage from the immersion film region, and an apparatus for supplying the immersion fluid (auto-immersion) to the immersion film region. The protection device is arranged statically in the main part of the microscope stand and the means for supplying immersion fluid to the immersion film region is connected to the protection device by means of an Aquastop system.

Abstract: A device for forming an immersion film between a front lens of a microscope objective and a preparation on an inverted microscope. The device includes a protection device for preventing fluid leakage from the immersion film region, and an apparatus for supplying the immersion fluid (auto-immersion) to the immersion film region. The protection device is arranged statically in the main part of the microscope stand and the means for supplying immersion fluid to the immersion film region is connected to the protection device by means of an Aquastop system.

Abstract: A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process.

Abstract: A procedure and a device for terminating the immersion at a microscope having one or more immersion lenses, or both immersion lenses and dry lenses, comprising a protective sheath around the immersion film area for preventing the escape of immersion media and an immersion media supply unit and an immersion media discharge unit connected with the protective sheath. The immersion media is removed in a controlled manner in particular in an automated microscope. Subsequently, the microscopic testing can be continued without the user having to intervene in the device or the process.

Abstract: A procedure and a device for terminating the immersion at a microscope having one or more immersion lenses, or both immersion lenses and dry lenses, comprising a protective sheath around the immersion film area for preventing the escape of immersion media and an immersion media supply unit and an immersion media discharge unit connected with the protective sheath. The immersion media is removed in a controlled manner in particular in an automated microscope. Subsequently, the microscopic testing can be continued without the user having to intervene in the device or the process.

Abstract: A device for sterilizing a sample chamber in an automated live cell microscope, having a sample holding frame contained in a housing of the microscope. The device includes a UVC sterilizing unit that emits an ultraviolet light with a wavelength of 280 nm to 100 nm (UVC radiation). The UVC sterilizing unit is arranged so that it is able to travel through the sample chamber for sterilization purposes.

Abstract: A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process.

Abstract: A device for forming an immersion film between a front lens of a microscope objective and a preparation on an inverted microscope. The device includes a protection device for preventing fluid leakage from the immersion film region, and an apparatus for supplying the immersion fluid (auto-immersion) to the immersion film region. The protection device is arranged statically in the main part of the microscope stand and the means for supplying immersion fluid to the immersion film region is connected to the protection device by means of an Aquastop system.

Abstract: A device for sterilizing a sample chamber in an automated live cell microscope, having a sample holding frame contained in a housing of the microscope. The device includes a UVC sterilizing unit that emits an ultraviolet light with a wavelength of 280 nm to 100 nm (UVC radiation). The UVC sterilizing unit is arranged so that it is able to travel through the sample chamber for sterilization purposes.