Abstract: The present invention provides in vitro models of the in vivo rolling and arrest of leukocytes along the endothelial cell wall, which are important steps in the migration of leukocytes out of the blood stream and into tissue, as part of the inflammatory response. The in vitro models of the invention are functional under physiologic flow conditions resulting in physiologic shear stresses. In a specific embodiment, for modelling leukocyte rolling, the apparatus of the invention comprises a solid phase surface with rolling mediator molecules present thereon. Such rolling mediators are, for example, selectins and selectin ligands which have binding partners expressed on leukocytes. In another specific embodiment, for modelling leukocyte rolling followed by adhesion/arrest, the apparatus of the invention comprises a solid phase surface with both rolling mediators and integrin binding partners present thereon.

Abstract: This invention concerns the cloning of the alpha-subunit of the p150,95 leukocyte adhesion receptor molecule. The invention also concerns the alpha-subunit of p150,95, as well as fragments of this receptor molecule. The invention further pertains to the diagnostic and therapeutic uses for such molecules.

Abstract: Pharmaceutical compositions comprising antibodies to intercellular adhesion molecule-1 (ICAM-1 or CD54) are useful in methods of decreasing the severity of inflammation associated with the adhesion of leukocytes to cells bearing ICAM-1. Treatment with anti-ICAM-1 antibodies reduced the severity of inflammation associated with acute organ or tissue rejection and prolonged allograft survival time. Such compositions may optionally contain other immunsuppressive agents.

Abstract: A method of purifying LFA-3 using affinity chromatography. Purified LFA-3 is useful for quantitating or separating out CD-2-containing cells.

Abstract: A micelle of an adhesion protein which naturally includes a phosphatidylinositol lipid anchor, the micelle being capable of binding multivalently to a plurality of target molecules on a cell surface; where the adhesion protein is LFA-3, the LFA-3 micelles can be administered to a patient to inhibit binding of activated T-cells to other cells.

Abstract: Polypeptides that bind to CD2, the receptor on the surface of T-lymphocytes. Most preferably, the polypeptides bind to CD2 on the surface of T-lymphocytes and inhibit adhesion between T-lymphocytes and target cells. DNA sequences that code on expression in appropriate unicellular hosts for those polypeptides. Methods of making and using those polypeptides in therapy and diagnosis.

Abstract: Methods featuring, in one aspect, a method of preparing an antigen or mixture of antigens substantially free of antigens specific to at least two monoclonal antibodies, said method involving contacting an antigen mixture with one or more previously-isolated monoclonal antibodies to form complexes between those antibodies and antigens present in the mixture specific to the antibodies, removing the complexes from the antigen mixture to yield a partially purified antigen mixture, immunizing an animal with the partially purified antigen mixture, fusing spleen cells from the immunized animal to myeloma cells to form hybridomas capable of producing additional monoclonal antibodies, culturing said hybridomas to produce said additional monoclonal antibodies, contacting a sample of the partially purified antigen mixture with said additional monoclonal antibodies to form complexes between said additional monoclonal antibodies and antigens present in the antigen mixture specific to the additional monoclonal antibodies,