Patents by Inventor Timothy C. Dodge
Timothy C. Dodge has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10266832Abstract: Described are compositions and methods relating variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.Type: GrantFiled: August 25, 2011Date of Patent: April 23, 2019Assignee: DANISCO US INCInventors: Timothy C. Dodge, Aleksandra Virag, Michael Ward
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Publication number: 20180148683Abstract: Described herein is a composition useful for inducing expression of genes whose expression is under control of an inducible promoter sequence and methods for the compositions preparation and use.Type: ApplicationFiled: July 7, 2016Publication date: May 31, 2018Applicant: DANISCO US INC.Inventors: Timothy C. DODGE, Ilkka Ilari KRUUS, Colin MITCHINSON, Timo Tapio VILJAVA
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Publication number: 20140024098Abstract: The invention provides methods for expression of a catalase enzyme in a Trichoderma host cell. In one embodiment, the catR gene from Aspergillus niger is expressed in Trichoderma reesei, resulting in improved yields of catalase enzyme in comparison with expression of catR in A. niger.Type: ApplicationFiled: July 19, 2013Publication date: January 23, 2014Applicant: Danisco US Inc.Inventors: Timothy C. Dodge, Katherine Marie Hoffmann, Andrei Miasnikov, Michael Ward
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Publication number: 20130224864Abstract: Described are compositions and methods relating variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.Type: ApplicationFiled: August 25, 2011Publication date: August 29, 2013Applicant: DANISCO US INC.Inventors: Timothy C. Dodge, Aleksandra Virag, Michael Ward
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Publication number: 20120009635Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.Type: ApplicationFiled: September 9, 2011Publication date: January 12, 2012Applicant: Danisco US Inc.Inventors: Timothy C. Dodge, Manoj Kumar, M. Harunur Rashid, Fernando Valle
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Patent number: 8034583Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.Type: GrantFiled: June 18, 2008Date of Patent: October 11, 2011Assignee: Danisco US Inc.Inventors: Timothy C. Dodge, Manoj Kumar, M. Harunur Rashid, Fernando Valle
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Publication number: 20110136197Abstract: The invention provides methods for expression of a catalase enzyme in a Trichoderma host cell. In one embodiment, the catR gene from Aspergillus niger is expressed in Trichoderma reesei, resulting in improved yields of catalase enzyme in comparison with expression of catR in A. niger.Type: ApplicationFiled: March 5, 2009Publication date: June 9, 2011Inventors: Timothy C Dodge, Katherine M. Hoffmann, Andrei Miasnikov, Michael Ward
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Publication number: 20080299614Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.Type: ApplicationFiled: June 18, 2008Publication date: December 4, 2008Inventors: Timothy C. Dodge, Manoj Kumar, M. Harunur Rashid, Fernando Valle
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Publication number: 20080254523Abstract: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.Type: ApplicationFiled: February 27, 2008Publication date: October 16, 2008Inventors: Timothy C. Dodge, Fernando Valle
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Patent number: 7419795Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.Type: GrantFiled: May 10, 2004Date of Patent: September 2, 2008Assignee: Danisco A/S, Genencor DivisionInventors: Timothy C. Dodge, Manoj Kumar, M. Harunur Rashid, Fernando Valle
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Patent number: 7407780Abstract: The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.Type: GrantFiled: November 13, 2006Date of Patent: August 5, 2008Assignee: Genencor International, Inc.Inventors: Timothy C. Dodge, Fernando Valle
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Patent number: 7241587Abstract: The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.Type: GrantFiled: April 4, 2002Date of Patent: July 10, 2007Assignee: Genencor International, Inc.Inventors: Timothy C. Dodge, Fernando Valle
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Patent number: 7033804Abstract: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.Type: GrantFiled: April 4, 2002Date of Patent: April 25, 2006Assignee: Genencor International, Inc.Inventors: Timothy C. Dodge, Fernando Valle
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Publication number: 20030073200Abstract: The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.Type: ApplicationFiled: April 4, 2002Publication date: April 17, 2003Inventors: Timothy C. Dodge, Fernando Valle
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Publication number: 20030040086Abstract: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.Type: ApplicationFiled: April 5, 2002Publication date: February 27, 2003Inventors: Timothy C. Dodge, Fernando Valle
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Patent number: 6303354Abstract: There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.Type: GrantFiled: November 21, 2000Date of Patent: October 16, 2001Assignee: Genencor International, Inc.Inventors: Walter Weyler, Timothy C. Dodge, John J. Lauff, Dan J. Wendt
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Patent number: 6190892Abstract: There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.Type: GrantFiled: November 20, 1995Date of Patent: February 20, 2001Assignee: Genencor International, Inc.Inventors: Walter Weyler, Timothy C. Dodge, John J. Lauff, Dan J. Wendt
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Patent number: 5866396Abstract: There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.Type: GrantFiled: December 11, 1996Date of Patent: February 2, 1999Inventors: Walter Weyler, Timothy C. Dodge, John J. Lauff, Dan J. Wendt