Patents by Inventor Timothy D. Leathers
Timothy D. Leathers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10612063Abstract: The present disclosure provides methods for producing bioproducts from novel genetically altered strains of Aureobasidium pullulans. Methods and materials for the construction of these strains, examination of the bioproducts and analysis and isolation of the bioproducts from genetically altered strains is provided. Genetically altered A. pullulans strains in which one or more genes encoding biosynthetic enzymes are knocked out is detailed and the benefits of using such strains described.Type: GrantFiled: May 8, 2019Date of Patent: April 7, 2020Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Christopher D. Skory, Timothy D. Leathers, Neil P. Price
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Patent number: 10544085Abstract: Compounds, called liamocins from Aureobasidium pullulans, having the general structure in Formula 1 are disclosed. where R1 is either COCH3 or H; and R2 is between two to ten O-linked 3,5-dihydroxydecanoate; and R3 can be a polyol (e.g., L- or D-glycerol, L- or D-threitol, L- or D-erythritol, L- or D-arabitol, L- or D-xylitol, L- or D-lyxitol, L- or D-ribitol, L- or D-allitol, L- or D-altritol, L- or D-mannitol, L- or D-iditol, L- or D-gulitol, L- or D-glucitol (also called sorbitol), L- or D-galactitol (also called dulcitol), and L- or D-talitol), 2-amino-D-mannitol, 2N-acetylamino-D-mannitol, L-rhamnitol, or D-fucitol; except when R3 is D-mannitol, R2 is not 2 nor 3 O-linked 3,5-dihydroxydecanoate chains. These liamocins described above in addition to D-mannitol liamocin A1, D-mannitol liamocin A2, D-mannitol liamocin B1, and D-mannitol liamocin B2, alone or in combination with each other, can be used to kill certain bacteria and to treat certain bacterial infections.Type: GrantFiled: March 13, 2018Date of Patent: January 28, 2020Assignee: The United States of America, as Represented by the Secretary of AgricultureInventors: Kenneth M. Bischoff, Timothy D. Leathers, Neil P. Price, Pennapa Manitchotpisit
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Publication number: 20190256879Abstract: The present disclosure provides methods for producing bioproducts from novel genetically altered strains of Aureobasidium pullulans. Methods and materials for the construction of these strains, examination of the bioproducts and analysis and isolation of the bioproducts from genetically altered strains is provided. Genetically altered A. pullulans strains in which one or more genes encoding biosynthetic enzymes are knocked out is detailed and the benefits of using such strains described.Type: ApplicationFiled: May 8, 2019Publication date: August 22, 2019Inventors: Christopher D. Skory, Timothy D. Leathers, NEIL P. PRICE
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Patent number: 10351889Abstract: The present disclosure provides methods for producing bioproducts from novel genetically altered strains of Aureobasidium pullulans. Methods and materials for the construction of these strains, examination of the bioproducts and analysis and isolation of the bioproducts from genetically altered strains is provided. Genetically altered A. pullulans strains in which one or more genes encoding biosynthetic enzymes are knocked out is detailed and the benefits of using such strains described.Type: GrantFiled: July 18, 2016Date of Patent: July 16, 2019Assignee: The United States of America, as represented by The Secretary of AgricultureInventors: Christopher D. Skory, Timothy D. Leathers, Neil P Price
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Publication number: 20180201571Abstract: Compounds, called liamocins from Aureobasidium pullulans, having the general structure in Formula 1 are disclosed. where R1 is either COCH3 or H; and R2 is between two to ten O-linked 3,5-dihydroxydecanoate; and R3 can be a polyol (e.g., L- or D-glycerol, L- or D-threitol, L- or D-erythritol, L- or D-arabitol, L- or D-xylitol, L- or D-lyxitol, L- or D-ribitol, L- or D-allitol, L- or D-altritol, L- or D-mannitol, L- or D-iditol, L- or D-gulitol, L- or D-glucitol (also called sorbitol), L- or D-galactitol (also called dulcitol), and L- or D-talitol), 2-amino-D-mannitol, 2N-acetylamino-D-mannitol, L-rhamnitol, or D-fucitol; except when R3 is D-mannitol, R2 is not 2 nor 3 O-linked 3,5-dihydroxydecanoate chains. These liamocins described above in addition to D-mannitol liamocin A1, D-mannitol liamocin A2, D-mannitol liamocin B1, and D-mannitol liamocin B2, alone or in combination with each other, can be used to kill certain bacteria and to treat certain bacterial infections.Type: ApplicationFiled: March 13, 2018Publication date: July 19, 2018Inventors: Kenneth M. Bischoff, Timothy D. Leathers, Neil P. Price, Pennapa Manitchotpisit
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Patent number: 9950986Abstract: Novel compounds, called liamocins from Aureobasidium pullulans, having the general structure in Formula 1 are disclosed. where R1 is either COCH3 or H; and R2 is between two to ten O-linked 3,5-dihydroxydecanoate; and R3 can be a polyol (e.g., L- or D-glycerol, L- or D-threitol, L- or D-erythritol, L- or D-arabitol, L- or D-xylitol, L- or D-lyxitol, L- or D-ribitol, L- or D-allitol, L- or D-altritol, L- or D-mannitol, L- or D-iditol, L- or D-gulitol, L- or D-glucitol (also called sorbitol), L- or D-galactitol (also called dulcitol), and L- or D-talitol), 2-amino-D-mannitol, 2N-acetylamino-D-mannitol, L-rhamnitol, or D-fucitol; except when R3 is D-mannitol, R2 is not 2 nor 3 O-linked 3,5-dihydroxydecanoate chains. These liamocins described above in addition to D-mannitol liamocin A1, D-mannitol liamocin A2, D-mannitol liamocin B1, and D-mannitol liamocin B2, alone or in combination with each other, can be used to kill certain bacteria and to treat certain bacterial infections.Type: GrantFiled: March 16, 2015Date of Patent: April 24, 2018Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Kenneth M. Bischoff, Timothy D. Leathers, Neil P. Price, Pennapa Manitchotpisit
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Publication number: 20170050916Abstract: Novel compounds, called liamocins from Aureobasidium pullulans, having the general structure in Formula 1 are disclosed. where R1 is either COCH3 or H; and R2 is between two to ten O-linked 3,5-dihydroxydecanoate; and R3 can be a polyol (e.g., L- or D-glycerol, L- or D-threitol, L- or D-erythritol, L- or D-arabitol, L- or D-xylitol, L- or D-lyxitol, L- or D-ribitol, L- or D-allitol, L- or D-altritol, L- or D-mannitol, L- or D-iditol, L- or D-gulitol, L- or D-glucitol (also called sorbitol), L- or D-galactitol (also called dulcitol), and L- or D-talitol), 2-amino-D-mannitol, 2N-acetylamino-D-mannitol, L-rhamnitol, or D-fucitol; except when R3 is D-mannitol, R2 is not 2 nor 3 O-linked 3,5-dihydroxydecanoate chains. These liamocins described above in addition to D-mannitol liamocin A1, D-mannitol liamocin A2, D-mannitol liamocin B1, and D-mannitol liamocin B2, alone or in combination with each other, can be used to kill certain bacteria and to treat certain bacterial infections.Type: ApplicationFiled: March 16, 2015Publication date: February 23, 2017Inventors: Kenneth M. BISCHOFF, Timothy D. LEATHERS, Neil P. PRICE, Pennapa MANITCHOTPISIT
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Publication number: 20170016039Abstract: The present disclosure provides methods for producing bioproducts from novel genetically altered strains of Aureobasidium pullulans. Methods and materials for the construction of these strains, examination of the bioproducts and analysis and isolation of the bioproducts from genetically altered strains is provided. Genetically altered A. pullulans strains in which one or more genes encoding biosynthetic enzymes are knocked out is detailed and the benefits of using such strains described.Type: ApplicationFiled: July 18, 2016Publication date: January 19, 2017Inventors: Christopher D. Skory, Timothy D. Leathers, NEIL P. PRICE
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Publication number: 20160355847Abstract: The present disclosure is directed to methods for producing butyric acid comprising fermenting a lignocellulosic biomass hydrolysate using C. tyrobutyricum strain RPT-4213 under anaerobic conditions using dilute acid-pretreated hydrolysates of wheat straw, corn fiber, corn stover, rice hull, and switchgrass, for example.Type: ApplicationFiled: June 3, 2016Publication date: December 8, 2016Inventors: Siqing Liu, Kenneth M. Bischoff, Timothy D. Leathers, Joseph O. Rich
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Patent number: 7442528Abstract: Four new Penicillium spp. isolates (NRRL 21966, NRRL 21967, NRRL 21968, and NRRL 21969) are capable of essentially quantitative conversion of native alternan to a polymeric modified form having a lower apparent molecular weight than native alternan. A fifth isolate (NRRL 30489) obtained from a survey of deposited organisms and classified as a Penicillium subgenus Biverticillium has the same ability. The modified alternan has rheological properties similar to ultrasonicated alternan and is produced without the expense of ultrasonication. It would have utility as a substitute for gum arabic for uses such as bulking agents and extenders in foods and cosmetics.Type: GrantFiled: March 23, 2006Date of Patent: October 28, 2008Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Timothy D. Leathers, Melinda S. Nunnally, Gregory L. Cote
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Patent number: 7049105Abstract: Four new Penicillium spp. isolates (NRRL 21966, NRRL 21967, NRRL 21968, and NRRL 21969) are capable of essentially quantitative conversion of native alternan to a polymeric modified form having a lower apparent molecular weight than native alternan. A fifth isolate (NRRL 30489) obtained from a survey of deposited organisms and classified as a Penicillium subgenus Biverticillium has the same ability. The modified alternan has rheological properties similar to ultrasonicated alternan and is produced without the expense of ultrasonication. It would have utility as a substitute for gum arabic for uses such as bulking agents and extenders in foods and cosmetics.Type: GrantFiled: September 5, 2002Date of Patent: May 23, 2006Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Timothy D. Leathers, Melinda S. Nunnally, Gregory L. Cote
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Patent number: 6696282Abstract: The instant invention is drawn towards transformed strains of Fusarium sporotrichioides effective for the production of lycopene. The transformed strains comprise an expression cassette having three genes encoding, respectively, geranylgeranyl-pyrophosphate synthase, phytoene synthase and phytoene desaturase (i.e. Tri5crtE, Tri5crtB and Tri5crtl). The transformed strains of Fusarium sporotrichioides of the instant invention produce lycopene at levels of up to 0.5 milligrams per gram culture dry weight.Type: GrantFiled: January 14, 2002Date of Patent: February 24, 2004Assignee: The United States of America as represented by the Secretary of AgricultureInventors: James D. Jones, Thomas M. Hohn, Timothy D. Leathers
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Publication number: 20030022373Abstract: A method which combines the use of polymerase chain reaction (PCR) or oligonucleotide linkers and restriction enzymes which cleave degenerate or variable recognition site sequences to allow the cloning of multiple DNA sequences into a vector is disclosed. In this invention, a plurality of unrelated DNA sequences may be directionally cloned within a single vector by adding onto the ends of the sequences, restriction sites with specific sequences which are cleaved by corresponding restriction endonucleases which recognize degenerate or variable recognition sites and which generate cohesive ends upon cleavage. The compatibility (or ability to anneal) of the cohesive ends on different DNA sequences is controlled by the choice of the nucleotide sequence within the recognition sequences of the restriction endonucleases, allowing the DNA sequences to be inserted or joined in any desired orientation.Type: ApplicationFiled: January 14, 2002Publication date: January 30, 2003Inventors: James D. Jones, Thomas M. Hohn, Timothy D. Leathers
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Publication number: 20030022319Abstract: Four new Penicillium spp. isolates (NRRL 21966, NRRL 21967, NRRL 21968, and NRRL 21969) are capable of essentially quantitative conversion of native alternan to a polymeric modified form having a lower apparent molecular weight than native alternan. A fifth isolate (NRRL 30489) obtained from a survey of deposited organisms and classified as a Penicillium subgenus Biverticillium has the same ability. The modified alternan has rheological properties similar to ultrasonicated alternan and is produced without the expense of ultrasonication. It would have utility as a substitute for gum arabic for uses such as bulking agents and extenders in foods and cosmetics.Type: ApplicationFiled: September 5, 2002Publication date: January 30, 2003Applicant: The United States of America, as represented by the Secretary of AgricultureInventors: Timothy D. Leathers, Melinda S. Nunnally, Gregory L. Cote
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Patent number: 6479275Abstract: Four new Penicillium spp. isolates (NRRL 21966, NRRL 21967, NRRL 21968, and NRRL 21969) are capable of essentially quantitative conversion of native alternan to a polymeric modified form having a lower apparent molecular weight than native alternan. A fifth isolate (NRRL 30489) obtained from a survey of deposited organisms and classified as a Penicillium subgenus Biverticillium has the same ability. The modified alternan has rheological properties similar to ultrasonicated alternan and is produced without the expense of ultrasonication. It would have utility as a substitute for gum arabic for uses such as bulking agents and extenders in foods and cosmetics.Type: GrantFiled: July 25, 2001Date of Patent: November 12, 2002Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Timothy D. Leathers, Melinda S. Nunnally, Gregory L. Cote
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Patent number: 6372479Abstract: The instant invention is drawn towards transformed strains of Fusarium sporotrichioides effective for the production of B-carotene. The transformed strains comprise an expression cassette having four genes encoding, respectively, geranylgeranyl-pyrophosphate synthase, phytoene synthase, phytoene desaturase and lycopene cyclase (i.e. Tri5crtE, Tri5crtB, TriScrtI and CrtY). The transformed strains of Fusarium sporotrichioides of the instant invention produce B-carotene at levels of up to 3-4 milligrams per gram of fungus (dry weight).Type: GrantFiled: September 6, 2000Date of Patent: April 16, 2002Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: James D. Jones, Thomas M. Hohn, Timothy D. Leathers
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Patent number: 6184000Abstract: A method which combines the use of polymerase chain reaction (PCR) or oligonucleotide linkers and restriction enzymes which cleave degenerate or variable recognition site sequences to allow the cloning of multiple DNA sequences into a vector is disclosed. In this invention, a plurality of unrelated DNA sequences may be directionally cloned within a single vector by adding onto the ends of the sequences, restriction sites with specific sequences which are cleaved by corresponding restriction endonucleases which recognize degenerate or variable recognition sites and which generate cohesive ends upon cleavage. The compatibility (or ability to anneal) of the cohesive ends on different DNA sequences is controlled by the choice of the nucleotide sequence within the recognition sequences of the restriction endonucleases, allowing the DNA sequences to be inserted or joined in any desired orientation.Type: GrantFiled: July 23, 1999Date of Patent: February 6, 2001Assignee: The United States of America as represented by the Secretary of AgricultureInventors: James D. Jones, Thomas M. Hohn, Timothy D. Leathers
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Patent number: 5789209Abstract: A rapid screening procedure to select microorganism strains that produce a high proportion of alternan to dextan and a high proportion of alternansucrase to dextransucrase is described. Strains selected by the method are useful to produce a high proportion of alternansucrase to dextransucrase and to produce a high proportion of alternan to dextran, either fermentatively by cultivation of the strains on sucrose, or enzymatically by using alternansucrase produced by the strains of the invention. The selected strains can also be used to obtain microorganism strains having further improvements.Type: GrantFiled: April 29, 1996Date of Patent: August 4, 1998Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Timothy D. Leathers, G. Thomas Hayman, Gregory L. Cote
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Patent number: 5702942Abstract: A rapid screening procedure to select microorganism strains that produce a high proportion of alternan to dextran and a high proportion of alternansucrase to dextransucrase is described. Strains selected by the method are useful to produce a high proportion of alternansucrase to dextransucrase and to produce a high proportion of alternan to dextran, either fermentatively by cultivation of the strains on sucrose, or enzymatically by using alternansucrase produced by the strains of the invention. The selected strains can also be used to obtain microorganism strains having further improvements.Type: GrantFiled: June 6, 1995Date of Patent: December 30, 1997Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Timothy D. Leathers, G. Thomas Hayman, Gregory L. Cote