Abstract: Microscopy methods for determining embryo viability are described. A method can include accessing, at a compute device, fluorescence lifetime imaging microscopy (FLIM) data set associated with a biological material. The biological material can include either an embryo or a gamete. The method further includes extracting a fluorescence photon arrival time from a subset of data from the FLIM data set. The method further includes estimating a likelihood that the biological material will produce a successful pregnancy and/or a live birth based on the fluorescence photon arrival time histogram and an estimation model that has been trained using artificial intelligence and labeled clinical training data. The method includes generating an output signal representing the estimated likelihood that the biological material will produce a successful pregnancy and/or a live birth.

Abstract: The present invention generally relates to non-linear imaging systems and methods for use in various assisted reproductive technologies and other applications. In one aspect, the present invention is generally directed to two-photon and other non-linear microscope techniques that can be used to determine the status or maturity state of eggs or egg follicles, for example by determining the presence of polar bodies within the egg follicles. In some embodiments, endogenous metabolites, such as NADH or FAD, can be determined, and in some cases without externally altering those metabolite concentrations or without adding other compounds. Other embodiments of the invention are also directed to computer programs or systems that can be used to determine the status or maturity state of eggs or egg follicles, kits involving such systems, or the like.

Abstract: The present invention generally relates to non-linear imaging systems and methods for use in various assisted reproductive technologies and other applications. In one aspect, the present invention is generally directed to two-photon and other non-linear microscope techniques that can be used to determine the status or maturity state of eggs or egg follicles, for example by determining the presence of polar bodies within the egg follicles. In some embodiments, endogenous metabolites, such as NADH or FAD, can be determined, and in some cases without externally altering those metabolite concentrations or without adding other compounds. Other embodiments of the invention are also directed to computer programs or systems that can be used to determine the status or maturity state of eggs or egg follicles, kits in such systems, or the like.

Abstract: The present invention generally relates to non-linear imaging systems and methods for use in various assisted reproductive technologies and other applications. In one aspect, the present invention is generally directed to two-photon and other non-linear microscope techniques that can be used to determine the status or maturity state of eggs or egg follicles, for example by determining the presence of polar bodies within the egg follicles. In some embodiments, endogenous metabolites, such as NADH or FAD, can be determined, and in some cases without externally altering those metabolite concentrations or without adding other compounds. Other embodiments of the invention are also directed to computer programs or systems that can be used to determine the status or maturity state of eggs or egg follicles, kits in such systems, or the like.

Abstract: Disclosed is an appliance for volatilizing active substances in either herbal material or fluid extract for inhalation, including a material vaporization surface with a plurality of convective air flow apertures, a conductive heater and a convective heater.