Abstract: A publish-subscribe messaging method may include identifying a definition of a transformation rule for transforming a message received from a publisher. The message may be received from the publisher at a broker. The message may identify a topic and may include message data. A determination may be made as to whether the message is associated with a transformation rule for transforming the message. The method may further include transforming the message as defined by the transformation rule and sending the transformed message to a destination.

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

Abstract: Functionality is disclosed herein for using a framework for a VR/AR application to utilize different services. In some configurations, a VR/AR application can utilize different services, such as an animation service, a multi-modal disambiguation service, a virtual platform service, a recognition service, an automatic speech recognition (ASR) service, a text-to-speech (TTS) service, a search service, as well as one or more other services. Instead of a developer of the VR/AR application having to develop programming code to implement features provided by one or more of services, the developer may utilize functionality of existing services that are available from a service provider network.

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

Abstract: Functionality is disclosed herein for using a framework for a VR/AR application to utilize different services. In some configurations, a VR/AR application can utilize different services, such as an animation service, a multi-modal disambiguation service, a virtual platform service, a recognition service, an automatic speech recognition (ASR) service, a text-to-speech (TTS) service, a search service, as well as one or more other services. Instead of a developer of the VR/AR application having to develop programming code to implement features provided by one or more of services, the developer may utilize functionality of existing services that are available from a service provider network.

Abstract: A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.

Abstract: Functionality is disclosed herein for using a framework for a VR/AR application to utilize different services. In some configurations, a VR/AR application can utilize different services, such as an animation service, a multi-modal disambiguation service, a virtual platform service, a recognition service, an automatic speech recognition (ASR) service, a text-to-speech (TTS) service, a search service, as well as one or more other services. Instead of a developer of the VR/AR application having to develop programming code to implement features provided by one or more of services, the developer may utilize functionality of existing services that are available from a service provider network.

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

Abstract: Technology for publish-subscribe message transformations is provided. In one example, a publish-subscribe messaging method may include identifying a definition of a transformation rule for transforming a message received from a publisher. The message may be received from the publisher at a broker. The message may identify a topic and may include message data. A determination may be made as to whether the message is associated with a transformation rule for transforming the message. The method may further include transforming the message as defined by the transformation rule and sending the transformed message to a destination.

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.