Abstract: A clutch alignment apparatus and method of aligning a dual clutch system in a continuously variable transmission that enables a user to measure the alignment of the primary clutch and secondary clutch when the system is under a load that is similar to that which is applied when the system is operating under normal operating conditions.

Abstract: A clutch alignment apparatus and method of aligning a dual clutch system in a continuously variable transmission that enables a user to measure the alignment of the primary clutch and secondary clutch when the system is under a load that is similar to that which is applied when the system is operating under normal operating conditions.

Abstract: A one piece fan shroud for a tractor has first and second faces, a frame with top, left and right sides, a bottom, and an upper wall extending from the top of the frame. The upper wall, left and right sides of the fan shroud are dimensioned to match the internal geometry and seal with an internal surface of a tractor hood. A rubber seal may extend around the upper wall and the left and right sides of the frame.

Abstract: A process for identifying proteinaceous protoxins expressed by Bacillus thuringiensis genes is disclosed. According to the process, daughter toxins are first generated by subjecting a protoxin-containing material, such as parasporal crystals of Bacillus thuringiensis, to limited proteolysis with a proteolytic enzyme in an aqueous suspension having a pH above 9.5. The daughter toxins are then separated by high performance anion-exchange liquid chromatography at a constant pH in excess of 10 in an increasing gradient of a salt, preferably sodium chloride. The gradient conditions, which are specific for the column used, are achieved by employing a series of buffers having increasing concentration of the salt and introduced at a predetermined time and rate. The procedure provides a chromatogram showing clearly identifiable peaks of toxins and permits therefore the qualitative and quantitative characterization of the original mixture and isolation of the individual toxins.

Abstract: A process for identifying proteinaceous protoxins expressed by Bacillus thuringiensis genes is disclosed. According to the process, daughter toxins are first generated by subjecting a protoxin-containing material, such as parasporal crystals of Bacillus thuringiensis, to limited proteolysis with a proteolytic enzyme in an aqueous suspension having a pH above 9.5. The daughter toxins are then separated by high performance anion-exchange liquid chromatography at a constant pH in excess of 10 in an increasing gradient of a salt, preferably sodium chloride. The gradient conditions, which are specific for the column used, are achieved by employing a series of buffers having increasing concentration of the salt and introduced at a predetermined time and rate. The procedure provides a chromatogram showing clearly identifiable peaks of toxins and permits therefore the qualitative and quantitative characterization of the original mixture and isolation of the individual toxins.