Patents by Inventor Timothy M. Woudenberg
Timothy M. Woudenberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190169684Abstract: The present disclosure provides systems and methods for sequencing nucleic acid molecules using tunneling labels. A sequence of a nucleic acid molecule may be identified with high accuracy using a chip comprising sensors, wherein each individual sensor may comprise at least two electrodes separated by a gap. The electrodes may be configured to generate at least one electrical signal upon binding of a tunneling label associated with a nucleotide. Epigenetic information can also be determined at the same time as a nucleic acid sequence.Type: ApplicationFiled: October 24, 2018Publication date: June 6, 2019Inventors: Mark F. OLDHAM, Eric S. NORDMAN, Timothy M. WOUDENBERG, Gaurav GOYAL, Masoud VAKILI, Toshihiko HONKURA, Sam WOO, Hisao KAWASAKI, Kazusuke MIHARA
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Patent number: 9205468Abstract: A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.Type: GrantFiled: November 30, 2010Date of Patent: December 8, 2015Assignee: Fluidigm CorporationInventors: Jing Wang, Timothy M. Woudenberg
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Publication number: 20130186433Abstract: A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.Type: ApplicationFiled: November 30, 2010Publication date: July 25, 2013Applicant: Fluidigm CorporationInventors: Jing Wang, Timothy M. Woudenberg
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Publication number: 20120316083Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.Type: ApplicationFiled: August 21, 2012Publication date: December 13, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Muhammad A. Sharaf, Timothy M. Woudenberg, Khairuzzaman Bashar Mullah
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Publication number: 20120172252Abstract: A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.Type: ApplicationFiled: March 15, 2012Publication date: July 5, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Robert C. Jones, Kenneth J. Livak
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Patent number: 8119423Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.Type: GrantFiled: April 7, 2006Date of Patent: February 21, 2012Assignee: Applied Biosystems, LLCInventors: Timothy M. Woudenberg, Michael Albin, Reid B. Kowallis, Yefim Raysberg, Robert P. Ragusa, Emily S. Winn-Deen
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Patent number: 8067226Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.Type: GrantFiled: May 10, 2006Date of Patent: November 29, 2011Assignee: Applied Biosystems, LLCInventors: Timothy M. Woudenberg, Michael Albin, Reid B. Kowallis, Yefim Raysberg, Robert P. Ragusa, Emily S. Winn-Deen
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Publication number: 20110223596Abstract: The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.Type: ApplicationFiled: February 18, 2011Publication date: September 15, 2011Applicant: Life Technologies CorporationInventors: Timothy M. WOUDENBERG, Dar Bahatt, Muhammad A. Sharaf, Timothy Z. Liu, Serguei Ermakov, Charles R. Connell, Jens J. Hyldig-Nielsen
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Publication number: 20110124002Abstract: The present invention provides, in one aspect, an apparatus that comprises a disc-shaped substrate defining (1) a central reservoir region, (2) a plurality of channels in fluid communication with, and emanating substantially radially from, the central reservoir region, the channels being coplanar with each other, and each channel having (i) a proximal end which is linked to the reservoir region, and (ii) a distal end, and preferably (3) for each channel, at least one chamber, and preferably three chambers, linked by a passageway in fluid communication with the distal end of that channel.Type: ApplicationFiled: June 29, 2010Publication date: May 26, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Reid B. KOWALLIS
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Publication number: 20100291548Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.Type: ApplicationFiled: March 12, 2007Publication date: November 18, 2010Applicant: Applera CorporationInventors: Muhammad A. Sharaf, Timothy M. Woudenberg, Khairuzzaman Bashar Mullah
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Patent number: 7833711Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.Type: GrantFiled: April 7, 2006Date of Patent: November 16, 2010Assignee: Applied Biosystems, LLCInventors: Timothy M. Woudenberg, Michael Albin, Reid B. Kowallis, Yefim Raysberg, Robert P. Ragusa, Emily S. Winn-Deen
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Publication number: 20100173293Abstract: A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.Type: ApplicationFiled: July 2, 2009Publication date: July 8, 2010Applicant: Life Technologies CorporationInventors: Timothy M. Woudenberg, Robert C. Jones, Kenneth J. Livak
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Publication number: 20100112572Abstract: The teachings herein generally relates to probes comprising fabricated coded molecular tags for detecting analytes. The teachings also relate to compositions, methods, and kits for fabricating coded molecular tags comprising a multiplicity or reporter groups in an ordered pattern.Type: ApplicationFiled: July 21, 2009Publication date: May 6, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Dar Bahatt, Muhammad A. Sharaf, Timothy Z. Liu, Serguei Ermakov, Charles R. Connell, Jens J. Hyldig-Nielsen, Benjamin Schroeder, Paolo Vatta
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Publication number: 20100105886Abstract: The present teachings generally relate to probes and probes sets for detecting analytes. The teachings also relates to compositions, methods, and kits for assembling probes comprising at least one coded molecular tag.Type: ApplicationFiled: July 21, 2009Publication date: April 29, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Dar Bahatt, Muhammad A. Sharaf, Timothy Z. Liu, Serguei Ermakov, Charles R. Connell
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Publication number: 20090325184Abstract: Compositions and methods of use are disclosed for clonally amplifying and analyzing one or more polynucleotides.Type: ApplicationFiled: September 8, 2009Publication date: December 31, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Cheryl Heiner, Eric S. Nordman
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Publication number: 20090317824Abstract: The present invention provides, in one aspect, an apparatus that comprises a disc-shaped substrate defining (1) a central reservoir region, (2) a plurality of channels in fluid communication with, and emanating substantially radially from, the central reservoir region, the channels being coplanar with each other, and each channel having (i) a proximal end which is linked to the reservoir region, and (ii) a distal end, and preferably (3) for each channel, at least one chamber, and preferably three chambers, linked by a passageway in fluid communication with the distal end of that channel.Type: ApplicationFiled: August 26, 2009Publication date: December 24, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Reid B. Kowallis
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Publication number: 20090280497Abstract: The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.Type: ApplicationFiled: June 26, 2009Publication date: November 12, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Timothy M. Woudenberg, Dar Bahatt, Muhammad A. Sharaf, Timothy Z. Liu, Serguei Ermakov, Charles R. Connell, Jens J. Hyldig-Nielsen
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Publication number: 20090155765Abstract: A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction.Type: ApplicationFiled: November 5, 2008Publication date: June 18, 2009Applicant: Applied Biosystems Inc.Inventors: John Girdner Atwood, Albert Carmelo Mossa, Lisa May Goven, Fenton Williams, Timothy M. Woudenberg, Marcel Margulies, Robert P. Rahusa, Richard Leath, Clive Miles
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Patent number: 7504241Abstract: A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction.Type: GrantFiled: May 12, 2006Date of Patent: March 17, 2009Assignee: Applied Biosystems, LLCInventors: John Girdner Atwood, Albert Carmelo Mossa, Lisa May Goven, Fenton Williams, Timothy M. Woudenberg, Marcel Margulies, Robert P. Ragusa, Richard Leath, Clive Miles
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Patent number: 7491305Abstract: The present invention provides, in one aspect, an apparatus or electrophoretic separation of analytes. In one aspect, the apparatus comprises a disc-shaped substrate defining (1) a central reservoir region, (2) a plurality of electrophoretic channels in fluid communication with, and emanating substantially radically from, the central reservoir region, the channels being coplanar with each other, and each channel having (i) a proximal end which is linked to the reservoir region, and (ii) a distal end, and preferably (3) for each channel, at least one chamber, and preferably three chambers, linked by a passageway in fluid communication with the distal end of that channel.Type: GrantFiled: December 8, 2003Date of Patent: February 17, 2009Assignee: Applied Biosystems Inc.Inventors: Timothy M. Woudenberg, Reid B. Kowallis