Abstract: Aspects of the present disclosure include methods for adjusting sensitivity of a photodiode in a light detection system. Methods according to certain embodiments include detecting light with a light detection system having a photodiode and an amplifier, determining responsivity of the photodiode over a plurality of wavelengths of light and adjusting one or more parameters of the amplifier in response to the responsivity of the photodiode over the plurality of wavelengths of light. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodiode and an amplifier for practicing the subject methods are also described. Non-transitory computer readable storage medium are also provided.

Abstract: Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include two or more photodetector arrays and an optical adjustment component positioned in an optical path between the photodetector arrays. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having two or more photodetector arrays and an optical adjustment component are also provided.

Abstract: Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include two or more photodetector arrays and an optical adjustment component positioned in an optical path between the photodetector arrays. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having two or more photodetector arrays and an optical adjustment component are also provided.

Abstract: Aspects of the present disclosure include a droplet deflector configured to apply a deflection force to a flow stream at a plurality of different angles. Droplet deflectors according to certain embodiments include one or more sets of parallel metallic plates that are configured to apply a deflection force to droplets in a flow stream flowing therebetween. Systems and methods for sorting particles in a sample with the subject deflector plates are also provided.

Abstract: Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include two or more photodetector arrays and an optical adjustment component positioned in an optical path between the photodetector arrays. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having two or more photodetector arrays and an optical adjustment component are also provided.

Abstract: Aspects of the present disclosure include methods and systems for assessing alignment of a light source with a flow stream. Methods according to certain embodiments include detecting first and second light signals along a vertical axis of the a light irradiated flow stream and calculating a differential signal amplitude between the first light signal and second light signal to assess the alignment of the light source with the flow stream. Systems for practicing the subject methods are also described.

Abstract: Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include two or more photodetector arrays and an optical adjustment component positioned in an optical path between the photodetector arrays. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having two or more photodetector arrays and an optical adjustment component are also provided.

Abstract: Aspects of the present disclosure include systems for dispensing a fluid, such as a sheath fluid, into a sample flow system of a flow cytometer. Systems according to certain embodiments include a housing and a pliant fluid dispensing container structure having a first pliant container and a second pliant container, where the second pliant container is configured to apply an amount of pressure to a fluid reservoir of the first pliant container sufficient to convey fluid from the fluid reservoir. Pliant fluid dispensing container structures and methods for using the subject systems to dispense a fluid, such as a sheath fluid to a sample flow system of a flow cytometer, are also described. Kits, having one or more of the pliant fluid dispensing container structures and sterile connector for coupling to a sample flow system are also provided.

Abstract: Aspects of the present disclosure include a droplet deflector configured to apply a deflection force to a flow stream at a plurality of different angles. Droplet deflectors according to certain embodiments include one or more sets of parallel metallic plates that are configured to apply a deflection force to droplets in a flow stream flowing therebetween. Systems and methods for sorting particles in a sample with the subject deflector plates are also provided.

Abstract: Aspects of the present disclosure include an optical detector scatter cap assembly. Optical detector scatter cap assemblies according to certain embodiments include a housing having a proximal end and a distal end, an orifice at the distal end of the housing, a scatter bar affixed to the housing and extending across the orifice and a cover bar that reversibly mates with the scatter bar. Also provided are systems that include the optical detector scatter cap assemblies, methods of using the systems, e.g., in sample processing, and kits that include component(s) of the optical detector scatter cap assemblies.

Abstract: Aspects of the present disclosure include methods for optically aligning components of a light collection system. Methods according to certain embodiments include coupling a connector having a first lens to an optical adjustment component having a second lens by connecting a first magnet and first aligner positioned on the connector to a second magnet and second aligner positioned on the optical adjustment component such that connecting the first magnet and first aligner to the second magnet and second aligner is sufficient to position the connector lens to be optically concentric with the optical adjustment component lens. Light collection systems optically aligned by the subject methods including flow cell nozzles optically aligned with an optical adjustment component are also described. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) are also provided.

Abstract: Aspects of the present disclosure include an optical detector scatter cap assembly. Optical detector scatter cap assemblies according to certain embodiments include a housing having a proximal end and a distal end, an orifice at the distal end of the housing, a scatter bar affixed to the housing and extending across the orifice and a cover bar that reversibly mates with the scatter bar. Also provided are systems that include the optical detector scatter cap assemblies, methods of using the systems, e.g., in sample processing, and kits that include component(s) of the optical detector scatter cap assemblies.

Abstract: Aspects of the present disclosure include methods and systems for assessing alignment of a light source with a flow stream. Methods according to certain embodiments include detecting first and second light signals along a vertical axis of the a light irradiated flow stream and calculating a differential signal amplitude between the first light signal and second light signal to assess the alignment of the light source with the flow stream. Systems for practicing the subject methods are also described.

Abstract: Aspects of the present disclosure include methods for optically aligning components of a light collection system. Methods according to certain embodiments include coupling a connector having a first lens to an optical adjustment component having a second lens by connecting a first magnet and first aligner positioned on the connector to a second magnet and second aligner positioned on the optical adjustment component such that connecting the first magnet and first aligner to the second magnet and second aligner is sufficient to position the connector lens to be optically concentric with the optical adjustment component lens. Light collection systems optically aligned by the subject methods including flow cell nozzles optically aligned with an optical adjustment component are also described. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) are also provided.

Abstract: Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.

Abstract: Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.

Abstract: Aspects of the present disclosure include a flow cell nozzle configured to propagate light emitted by a sample in a flow stream upstream by total internal reflectance. Flow cell nozzles according to certain embodiments include a nozzle chamber having a proximal end and a distal end and a nozzle orifice positioned at the distal end of the nozzle chamber where the flow cell nozzle is configured to propagate light emitted from a sample in the flow stream upstream through the flow cell nozzle orifice by total internal reflectance toward the proximal end of the nozzle chamber. Systems and methods employing the subject flow cell nozzles are also provided.

Abstract: Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.