Patents by Inventor Timothy Z. Liu

Timothy Z. Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11975329
    Abstract: An incubation system includes an actuator, a platform, an incubation lid, and a dispenser. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication, and the dispenser suspends over the thermal conductive plate of the platform.
    Type: Grant
    Filed: September 8, 2021
    Date of Patent: May 7, 2024
    Assignee: LifeOS Genomics Corporation
    Inventors: Timothy Z. Liu, Hung-Wen Chang
  • Publication number: 20230256438
    Abstract: A microfluidic cartridge includes lower cartridge body, a biochip, and an upper cartridge body. The lower cartridge body includes a first substrate and an inlet column. The inlet column is protruding above the substrate and is hollow. The biochip has a plurality of microwells and is attached to the first substrate of the lower cartridge body. The upper cartridge is disposed over the lower cartridge body and includes a second substrate, a first opening, and a first O-ring. The first opening penetrates the second substrate, wherein the inlet column of the lower cartridge body is inserted into the first opening, and the inlet column and the first opening are assembled into an inlet port. The first O-ring is disposed in the first opening. The inlet port and the biochip are connected to by an inflow channel.
    Type: Application
    Filed: January 31, 2023
    Publication date: August 17, 2023
    Applicant: LifeOS Genomics Corporation
    Inventors: Timothy Z. Liu, Cheng-Chang Lai
  • Publication number: 20210402409
    Abstract: An incubation system includes an actuator, a platform, an incubation lid, and a dispenser. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication, and the dispenser suspends over the thermal conductive plate of the platform.
    Type: Application
    Filed: September 8, 2021
    Publication date: December 30, 2021
    Applicant: LifeOS Genomics Corporation
    Inventors: Timothy Z. Liu, Hung-Wen Chang
  • Patent number: 11141726
    Abstract: A cartridge includes a plate including a fluid inlet and a fluid outlet, a biochip disposed under the plate, and a first adhesive layer bonding the plate and the biochip. A fluid channel is formed between the plate and the biochip. The fluid inlet and the fluid outlet are in fluid communication with the fluid channel.
    Type: Grant
    Filed: November 8, 2018
    Date of Patent: October 12, 2021
    Assignee: LifeOS Genomics Corporation
    Inventors: Cheng-Chang Lai, Hung-Wen Chang, Timothy Z. Liu, Ching-Jou Huang
  • Patent number: 10460828
    Abstract: A method of nucleic acid fragment detection includes capturing a target nucleic acid fragment by an oligonucleotide probe to form a hybridised double strand. The oligonucleotide probe has an identification sequence complementary to the target nucleic acid fragment and a reproducible sequence. The hybridised double strand is removed to expose the reproducible sequence of the oligonucleotide probe. The repeats of the reproducible sequence are produced. The repeats of the reproducible sequence are labelled by a detection probe for identification and quantitation.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: October 29, 2019
    Assignee: Lifeos Genomics Corporation
    Inventors: Timothy Z. Liu, Ching-Jou Huang
  • Publication number: 20190143322
    Abstract: A cartridge includes a plate including a fluid inlet and a fluid outlet, a biochip disposed under the plate, and a first adhesive layer bonding the plate and the biochip. A fluid channel is formed between the plate and the biochip. The fluid inlet and the fluid outlet are in fluid communication with the fluid channel.
    Type: Application
    Filed: November 8, 2018
    Publication date: May 16, 2019
    Inventors: Cheng-Chang Lai, Hung-Wen Chang, Timothy Z. Liu, Ching-Jou Huang
  • Patent number: 10280469
    Abstract: A method of quantifying multiple target nucleic acid sequences in a sample includes generating from a sample at least a plurality of first template molecules and at least a plurality of second template molecules. At least part of said first and at least part of said second template molecules are randomly distributed into individual reaction sites. A cluster of nucleic acid amplicons of said first template molecule and at least a cluster of nucleic acid amplicons of said second template molecule are generated by clonal amplification or replication. The ID codes of all said nucleic acid amplicon clusters are identified. The quantity of at least said first and second target nucleic acid sequences in said sample is quantified by statistical analysis of respective positive numbers of identified unique ID codes of first and second target nucleic acid sequences.
    Type: Grant
    Filed: October 18, 2016
    Date of Patent: May 7, 2019
    Assignee: LifeOS Genomics Corporation
    Inventor: Timothy Z. Liu
  • Publication number: 20180318839
    Abstract: An incubation device includes an actuator, a platform, and an incubation lid. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication.
    Type: Application
    Filed: May 3, 2017
    Publication date: November 8, 2018
    Inventors: Timothy Z. Liu, Hung-Wen Chang
  • Publication number: 20180096098
    Abstract: A method of nucleic acid fragment detection includes capturing a target nucleic acid fragment by an oligonucleotide probe to form a hybridised double strand. The oligonucleotide probe has an identification sequence complementary to the target nucleic acid fragment and a reproducible sequence. The hybridised double strand is removed to expose the reproducible sequence of the oligonucleotide probe. The repeats of the reproducible sequence are produced. The repeats of the reproducible sequence are labelled by a detection probe for identification and quantitation.
    Type: Application
    Filed: September 25, 2017
    Publication date: April 5, 2018
    Inventors: Timothy Z. Liu, Ching-Jou Huang
  • Publication number: 20170101687
    Abstract: A method of quantifying multiple target nucleic acid sequences in a sample includes generating from a sample at least a plurality of first template molecules and at least a plurality of second template molecules. At least part of said first and at least part of said second template molecules are randomly distributed into individual reaction sites. A cluster of nucleic acid amplicons of said first template molecule and at least a cluster of nucleic acid amplicons of said second template molecule are generated by clonal amplification or replication. The ID codes of all said nucleic acid amplicon clusters are identified. The quantity of at least said first and second target nucleic acid sequences in said sample is quantified by statistical analysis of respective positive numbers of identified unique ID codes of first and second target nucleic acid sequences.
    Type: Application
    Filed: October 18, 2016
    Publication date: April 13, 2017
    Inventor: Timothy Z. LIU
  • Patent number: 9534257
    Abstract: The present invention relates to methods and systems for single molecule based nucleic acid amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.
    Type: Grant
    Filed: December 6, 2013
    Date of Patent: January 3, 2017
    Assignee: LIFEOS GENOMICS CORPORATION
    Inventor: Timothy Z. Liu
  • Publication number: 20150038368
    Abstract: A method, apparatus, or system for generating a pattern of polynucleotides on a substrate. The method includes providing a substrate having a hydrophobic surface. The method further includes conjugating a polystyrene moiety to a polynucleotide and applying a polystyrene-polynucleotide conjugate to create a plurality of reaction spots on the hydrophobic surface of the substrate. An apparatus includes a substrate with at least one polystyrene-polynucleotide conjugate on a surface of the substrate. A system can analyze a polystyrene-polynucleotide conjugate and the system may perform PCR.
    Type: Application
    Filed: August 11, 2014
    Publication date: February 5, 2015
    Inventors: Timothy Z. LIU, Jer-Kang Chen, Ronald Graham
  • Publication number: 20140377750
    Abstract: Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
    Type: Application
    Filed: May 6, 2014
    Publication date: December 25, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Kristian M. Scaboo, Vissarion Aivazachvili, Timothy Z. Liu, Robert G. Eason, Konrad Faulstich
  • Patent number: 8883095
    Abstract: An analysis cartridge and an analysis system thereof are disclosed. The analysis cartridge comprises a cartridge body, a liquid storage box and a sealing film. The cartridge body has an accommodation portion. The liquid storage box is disposed within the accommodation portion and has a liquid storage tank and a pillar receiving hole. The sealing film covers the pillar receiving hole and seals an opening of the liquid storage tank.
    Type: Grant
    Filed: January 14, 2013
    Date of Patent: November 11, 2014
    Assignee: Lite-On Technology Corporation
    Inventors: Cheng-Chang Lai, Fu-Chun Huang, Yuh-Jiuan Lin, Timothy Z. Liu
  • Publication number: 20140093876
    Abstract: The present invention relates to methods and systems for single molecule based nucleic acid amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.
    Type: Application
    Filed: December 6, 2013
    Publication date: April 3, 2014
    Inventor: Timothy Z. Liu
  • Publication number: 20140045716
    Abstract: Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
    Type: Application
    Filed: August 7, 2013
    Publication date: February 13, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Kristian M. Scaboo, Vissarion Aivazachvili, Timothy Z. Liu, Robert G. Eason, Konrad Faulstich
  • Patent number: 8603743
    Abstract: The present invention relates to methods and systems for single molecule based clonal amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.
    Type: Grant
    Filed: October 3, 2011
    Date of Patent: December 10, 2013
    Inventor: Timothy Z. Liu
  • Publication number: 20130309133
    Abstract: An analysis cartridge and an analysis system thereof are disclosed. The analysis cartridge comprises a cartridge body, a liquid storage box and a sealing film. The cartridge body has an accommodation portion. The liquid storage box is disposed within the accommodation portion and has a liquid storage tank and a pillar receiving hole. The sealing film covers the pillar receiving hole and seals an opening of the liquid storage tank.
    Type: Application
    Filed: January 14, 2013
    Publication date: November 21, 2013
    Applicant: LITE-ON IT CORPORATION
    Inventors: Cheng-Chang LAI, Fu-Chun HUANG, Yuh-Jiuan LIN, Timothy Z. LIU
  • Publication number: 20120283109
    Abstract: This invention provides a method of analyzing target nucleic acids of biological samples for multiplex nucleic acid analysis of disease associated genetic changes of biological samples in biomedical research and clinical diagnostics.
    Type: Application
    Filed: April 9, 2012
    Publication date: November 8, 2012
    Inventor: Timothy Z. LIU
  • Publication number: 20120088684
    Abstract: The present invention relates to methods and systems for single molecule based clonal amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.
    Type: Application
    Filed: October 3, 2011
    Publication date: April 12, 2012
    Inventor: Timothy Z. Liu