Patents by Inventor Timothy Z. Liu
Timothy Z. Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11975329Abstract: An incubation system includes an actuator, a platform, an incubation lid, and a dispenser. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication, and the dispenser suspends over the thermal conductive plate of the platform.Type: GrantFiled: September 8, 2021Date of Patent: May 7, 2024Assignee: LifeOS Genomics CorporationInventors: Timothy Z. Liu, Hung-Wen Chang
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Publication number: 20230256438Abstract: A microfluidic cartridge includes lower cartridge body, a biochip, and an upper cartridge body. The lower cartridge body includes a first substrate and an inlet column. The inlet column is protruding above the substrate and is hollow. The biochip has a plurality of microwells and is attached to the first substrate of the lower cartridge body. The upper cartridge is disposed over the lower cartridge body and includes a second substrate, a first opening, and a first O-ring. The first opening penetrates the second substrate, wherein the inlet column of the lower cartridge body is inserted into the first opening, and the inlet column and the first opening are assembled into an inlet port. The first O-ring is disposed in the first opening. The inlet port and the biochip are connected to by an inflow channel.Type: ApplicationFiled: January 31, 2023Publication date: August 17, 2023Applicant: LifeOS Genomics CorporationInventors: Timothy Z. Liu, Cheng-Chang Lai
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Publication number: 20210402409Abstract: An incubation system includes an actuator, a platform, an incubation lid, and a dispenser. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication, and the dispenser suspends over the thermal conductive plate of the platform.Type: ApplicationFiled: September 8, 2021Publication date: December 30, 2021Applicant: LifeOS Genomics CorporationInventors: Timothy Z. Liu, Hung-Wen Chang
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Patent number: 11141726Abstract: A cartridge includes a plate including a fluid inlet and a fluid outlet, a biochip disposed under the plate, and a first adhesive layer bonding the plate and the biochip. A fluid channel is formed between the plate and the biochip. The fluid inlet and the fluid outlet are in fluid communication with the fluid channel.Type: GrantFiled: November 8, 2018Date of Patent: October 12, 2021Assignee: LifeOS Genomics CorporationInventors: Cheng-Chang Lai, Hung-Wen Chang, Timothy Z. Liu, Ching-Jou Huang
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Patent number: 10460828Abstract: A method of nucleic acid fragment detection includes capturing a target nucleic acid fragment by an oligonucleotide probe to form a hybridised double strand. The oligonucleotide probe has an identification sequence complementary to the target nucleic acid fragment and a reproducible sequence. The hybridised double strand is removed to expose the reproducible sequence of the oligonucleotide probe. The repeats of the reproducible sequence are produced. The repeats of the reproducible sequence are labelled by a detection probe for identification and quantitation.Type: GrantFiled: September 25, 2017Date of Patent: October 29, 2019Assignee: Lifeos Genomics CorporationInventors: Timothy Z. Liu, Ching-Jou Huang
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Publication number: 20190143322Abstract: A cartridge includes a plate including a fluid inlet and a fluid outlet, a biochip disposed under the plate, and a first adhesive layer bonding the plate and the biochip. A fluid channel is formed between the plate and the biochip. The fluid inlet and the fluid outlet are in fluid communication with the fluid channel.Type: ApplicationFiled: November 8, 2018Publication date: May 16, 2019Inventors: Cheng-Chang Lai, Hung-Wen Chang, Timothy Z. Liu, Ching-Jou Huang
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Patent number: 10280469Abstract: A method of quantifying multiple target nucleic acid sequences in a sample includes generating from a sample at least a plurality of first template molecules and at least a plurality of second template molecules. At least part of said first and at least part of said second template molecules are randomly distributed into individual reaction sites. A cluster of nucleic acid amplicons of said first template molecule and at least a cluster of nucleic acid amplicons of said second template molecule are generated by clonal amplification or replication. The ID codes of all said nucleic acid amplicon clusters are identified. The quantity of at least said first and second target nucleic acid sequences in said sample is quantified by statistical analysis of respective positive numbers of identified unique ID codes of first and second target nucleic acid sequences.Type: GrantFiled: October 18, 2016Date of Patent: May 7, 2019Assignee: LifeOS Genomics CorporationInventor: Timothy Z. Liu
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Publication number: 20180318839Abstract: An incubation device includes an actuator, a platform, and an incubation lid. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication.Type: ApplicationFiled: May 3, 2017Publication date: November 8, 2018Inventors: Timothy Z. Liu, Hung-Wen Chang
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Publication number: 20180096098Abstract: A method of nucleic acid fragment detection includes capturing a target nucleic acid fragment by an oligonucleotide probe to form a hybridised double strand. The oligonucleotide probe has an identification sequence complementary to the target nucleic acid fragment and a reproducible sequence. The hybridised double strand is removed to expose the reproducible sequence of the oligonucleotide probe. The repeats of the reproducible sequence are produced. The repeats of the reproducible sequence are labelled by a detection probe for identification and quantitation.Type: ApplicationFiled: September 25, 2017Publication date: April 5, 2018Inventors: Timothy Z. Liu, Ching-Jou Huang
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Publication number: 20170101687Abstract: A method of quantifying multiple target nucleic acid sequences in a sample includes generating from a sample at least a plurality of first template molecules and at least a plurality of second template molecules. At least part of said first and at least part of said second template molecules are randomly distributed into individual reaction sites. A cluster of nucleic acid amplicons of said first template molecule and at least a cluster of nucleic acid amplicons of said second template molecule are generated by clonal amplification or replication. The ID codes of all said nucleic acid amplicon clusters are identified. The quantity of at least said first and second target nucleic acid sequences in said sample is quantified by statistical analysis of respective positive numbers of identified unique ID codes of first and second target nucleic acid sequences.Type: ApplicationFiled: October 18, 2016Publication date: April 13, 2017Inventor: Timothy Z. LIU
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Patent number: 9534257Abstract: The present invention relates to methods and systems for single molecule based nucleic acid amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.Type: GrantFiled: December 6, 2013Date of Patent: January 3, 2017Assignee: LIFEOS GENOMICS CORPORATIONInventor: Timothy Z. Liu
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Publication number: 20150038368Abstract: A method, apparatus, or system for generating a pattern of polynucleotides on a substrate. The method includes providing a substrate having a hydrophobic surface. The method further includes conjugating a polystyrene moiety to a polynucleotide and applying a polystyrene-polynucleotide conjugate to create a plurality of reaction spots on the hydrophobic surface of the substrate. An apparatus includes a substrate with at least one polystyrene-polynucleotide conjugate on a surface of the substrate. A system can analyze a polystyrene-polynucleotide conjugate and the system may perform PCR.Type: ApplicationFiled: August 11, 2014Publication date: February 5, 2015Inventors: Timothy Z. LIU, Jer-Kang Chen, Ronald Graham
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Publication number: 20140377750Abstract: Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.Type: ApplicationFiled: May 6, 2014Publication date: December 25, 2014Applicant: Applied Biosystems, LLCInventors: Kristian M. Scaboo, Vissarion Aivazachvili, Timothy Z. Liu, Robert G. Eason, Konrad Faulstich
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Patent number: 8883095Abstract: An analysis cartridge and an analysis system thereof are disclosed. The analysis cartridge comprises a cartridge body, a liquid storage box and a sealing film. The cartridge body has an accommodation portion. The liquid storage box is disposed within the accommodation portion and has a liquid storage tank and a pillar receiving hole. The sealing film covers the pillar receiving hole and seals an opening of the liquid storage tank.Type: GrantFiled: January 14, 2013Date of Patent: November 11, 2014Assignee: Lite-On Technology CorporationInventors: Cheng-Chang Lai, Fu-Chun Huang, Yuh-Jiuan Lin, Timothy Z. Liu
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Publication number: 20140093876Abstract: The present invention relates to methods and systems for single molecule based nucleic acid amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.Type: ApplicationFiled: December 6, 2013Publication date: April 3, 2014Inventor: Timothy Z. Liu
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Publication number: 20140045716Abstract: Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.Type: ApplicationFiled: August 7, 2013Publication date: February 13, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Kristian M. Scaboo, Vissarion Aivazachvili, Timothy Z. Liu, Robert G. Eason, Konrad Faulstich
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Patent number: 8603743Abstract: The present invention relates to methods and systems for single molecule based clonal amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.Type: GrantFiled: October 3, 2011Date of Patent: December 10, 2013Inventor: Timothy Z. Liu
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Publication number: 20130309133Abstract: An analysis cartridge and an analysis system thereof are disclosed. The analysis cartridge comprises a cartridge body, a liquid storage box and a sealing film. The cartridge body has an accommodation portion. The liquid storage box is disposed within the accommodation portion and has a liquid storage tank and a pillar receiving hole. The sealing film covers the pillar receiving hole and seals an opening of the liquid storage tank.Type: ApplicationFiled: January 14, 2013Publication date: November 21, 2013Applicant: LITE-ON IT CORPORATIONInventors: Cheng-Chang LAI, Fu-Chun HUANG, Yuh-Jiuan LIN, Timothy Z. LIU
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Publication number: 20120283109Abstract: This invention provides a method of analyzing target nucleic acids of biological samples for multiplex nucleic acid analysis of disease associated genetic changes of biological samples in biomedical research and clinical diagnostics.Type: ApplicationFiled: April 9, 2012Publication date: November 8, 2012Inventor: Timothy Z. LIU
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Publication number: 20120088684Abstract: The present invention relates to methods and systems for single molecule based clonal amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.Type: ApplicationFiled: October 3, 2011Publication date: April 12, 2012Inventor: Timothy Z. Liu