Patents by Inventor Tobias Daniel Wheeler
Tobias Daniel Wheeler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11084036Abstract: Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.Type: GrantFiled: November 5, 2018Date of Patent: August 10, 2021Assignee: 10X Genomics, Inc.Inventors: Rajiv Bharadwaj, Kevin Ness, Tobias Daniel Wheeler
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Publication number: 20210222158Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.Type: ApplicationFiled: March 22, 2021Publication date: July 22, 2021Inventors: Zahra Kamila Belhocine, Rajiv Bharadwaj, Christopher Hindson, Michael Schnall-Levin, Bill Kengli Lin, Anthony Makarewicz, Pranav Patel, Katherine Pfeiffer, Andrew D. Price, Mohammad Rahimi Lenji, Tobias Daniel Wheeler, Yifeng Yin
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Publication number: 20210130892Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.Type: ApplicationFiled: October 29, 2020Publication date: May 6, 2021Inventors: Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas
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Publication number: 20210102936Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).Type: ApplicationFiled: June 9, 2020Publication date: April 8, 2021Inventors: Zahra Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
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Publication number: 20200391211Abstract: Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.Type: ApplicationFiled: April 27, 2020Publication date: December 17, 2020Inventors: Tobias Daniel WHEELER, Bill Kengli LIN, Mohammad Rahimi LENJI, Anthony MAKAREWICZ, Rajiv BHARADWAJ
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Publication number: 20200354771Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.Type: ApplicationFiled: April 22, 2020Publication date: November 12, 2020Inventors: Zahra Kamila Belhocine, Josephine Lee, Francesca Meschi, Luz Montesclaros, Katherine Pfeiffer, Andrew D. Price, Jerald Sapida, David Sukovich, Tobias Daniel Wheeler, Yifeng Yin
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Publication number: 20200290049Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.Type: ApplicationFiled: May 29, 2020Publication date: September 17, 2020Inventors: Rajiv BHARADWAJ, Tobias Daniel WHEELER, Kevin NESS, Benjamin HINDSON
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Patent number: 10717081Abstract: The invention provides microfluidic devices, systems, and methods for manipulating an object within a channel of a microfluidic device using an external electrode. The device has a channel disposed within the device, the channel having no included electrodes. The channel has a wall, at least a portion of which is penetrable by an electric field generated external to the device, the wall being penetrable such that the electric field extends through the wall portion and into a region within the channel. The system includes the microfluidic device and an electrode external to and not bonded to the device. In the method, the external electrode is placed adjacent to the device and energized to generate an electric field that extends through the wall of the device and into the channel, thereby manipulating an object within the channel.Type: GrantFiled: November 16, 2015Date of Patent: July 21, 2020Assignee: Caliper Life Sciences, Inc.Inventors: Joshua I. Molho, Daniel G. Stearns, I-Jane Chen, Danh Tran, Bradley W. Rice, Tobias Daniel Wheeler
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Patent number: 10688494Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.Type: GrantFiled: August 23, 2017Date of Patent: June 23, 2020Assignee: 10X Genomics, Inc.Inventors: Rajiv Bharadwaj, Tobias Daniel Wheeler, Kevin Ness, Benjamin Hindson
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Publication number: 20200165603Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.Type: ApplicationFiled: May 22, 2019Publication date: May 28, 2020Inventors: Kamila Belhocine, Rajiv Bharadwaj, Christopher Hindson, Michael Schnall-Levin, Bill Kengli Lin, Anthony Makarewicz, Pranav Patel, Katherine Pfeiffer, Andrew D. Price, Mohammad Rahimi Lenji, Tobias Daniel Wheeler, Yifeng Yin
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Publication number: 20200140944Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.Type: ApplicationFiled: November 22, 2019Publication date: May 7, 2020Inventors: Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohamad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo
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Publication number: 20200108389Abstract: A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.Type: ApplicationFiled: December 5, 2019Publication date: April 9, 2020Inventors: Tobias Daniel WHEELER, Rajiv BHARADWAJ
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Publication number: 20200024596Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.Type: ApplicationFiled: May 22, 2019Publication date: January 23, 2020Inventors: Kamila Belhocine, Rajiv Bharadwaj, Christopher Hindson, Michael Schnall-Levin, Bill Kengli Lin, Anthony Makarewicz, Pranav Patel, Katherine Pfeiffer, Andrew D. Price, Mohammad Rahimi Lenji, Tobias Daniel Wheeler, Yifeng Yin
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Publication number: 20200002763Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.Type: ApplicationFiled: June 12, 2019Publication date: January 2, 2020Inventors: Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas
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Publication number: 20200002764Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.Type: ApplicationFiled: June 17, 2019Publication date: January 2, 2020Inventors: Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas
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Publication number: 20190383804Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.Type: ApplicationFiled: April 4, 2019Publication date: December 19, 2019Inventors: Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
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Publication number: 20190383798Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.Type: ApplicationFiled: April 4, 2019Publication date: December 19, 2019Inventors: Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
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Publication number: 20190367969Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).Type: ApplicationFiled: April 4, 2019Publication date: December 5, 2019Inventors: Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
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Publication number: 20190367982Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications, including cell lineage analysis. Cell lineage analysis may comprise the use of one or more lineage tracing nucleic acid molecules. The disclosed methods may comprise using a lineage tracing nucleic acid molecule to identify a biological particle with one or more progenitor cells.Type: ApplicationFiled: April 4, 2019Publication date: December 5, 2019Inventors: Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliot Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Tery, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
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Publication number: 20190367966Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.Type: ApplicationFiled: April 4, 2019Publication date: December 5, 2019Inventors: Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez