Patents by Inventor Tobin Hellyer
Tobin Hellyer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9624555Abstract: Nucleic acid amplification primers and methods for specific detection of influenza A and influenza B nucleic acid targets are disclosed. The primer-target binding sequences are useful for detection of influenza A and influenza B targets in a variety of amplification and hybridization reactions. The oligonucleotide sequences are able to differentiate between influenza A and influenza B strains through specific hybridization to one or the other virus strain, enabling specific detection of the presence of influenza A and/or influenza B in a specimen.Type: GrantFiled: June 13, 2013Date of Patent: April 18, 2017Assignee: Becton, Dickinson and CompanyInventors: Tobin Hellyer, James A. Price, Jr., Erika L. Jones
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Patent number: 9499858Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.Type: GrantFiled: January 11, 2013Date of Patent: November 22, 2016Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, Tobin Hellyer, Dolores M. Berger, William Nussbaumer, Robert Rosenstein, Andrew Kuhn, Sha-Sha Wang, Keith Edward Thornton
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Patent number: 9217182Abstract: A full process control for use with a molecular assay and a method of determine the efficacy of the molecular assay. A full process control can include a fixed cell, and specifically can include a fixed vegetative cell. A method of determining the efficacy of a molecular assay can include providing an internal control, mixing the internal control with a sample, lysing the internal control and the sample, and detecting the lysis product. The full process control and/or the internal control can be Bacillus subtilis cells.Type: GrantFiled: November 7, 2013Date of Patent: December 22, 2015Assignee: Becton, Dickinson and CompanyInventors: Benoit Dore, Christian Menard, Tobin Hellyer
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Publication number: 20150152473Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.Type: ApplicationFiled: January 11, 2013Publication date: June 4, 2015Applicant: BECTON, DICKINSON AND COMPANYInventors: James G. Nadeau, Tobin Hellyer, Dolores M. Berger, William Nussbaumer, Robert Rosenstein, Andrew Kuhn, Sha-Sha Wang, Keith Edward Thornton
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Patent number: 8916349Abstract: Oligonucleotides for detection of nucleic acid in a sample that provide for an amplified signal by recycling probes and probe fragments.Type: GrantFiled: November 23, 2010Date of Patent: December 23, 2014Assignee: Becton, Dickinson and CompanyInventors: Jim Nadeau, Tobin Hellyer
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Patent number: 8859199Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: GrantFiled: April 4, 2008Date of Patent: October 14, 2014Assignee: Becton, Dickinson and CompanyInventors: Tobin Hellyer, Thomas Fort, Ray A. McMillian
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Publication number: 20140193819Abstract: Provided herein are methods and kits for modulating the amplification efficiency of nucleic acids, which are useful in multiplex reactions where the amplification efficiency of one or more nucleic acids in the mixture are desired to be modulated relative to one or more other nucleic acids. Embodiments relate to molecular diagnostics, including detecting sequence variants, such as SNPs, insertions deletions, and altered methylation patterns, as well as the modulation of the amplification efficiency of internal control sequences to provide more accurate control sequences for amplification reactions.Type: ApplicationFiled: March 11, 2014Publication date: July 10, 2014Applicant: Becton, Dickinson and CompanyInventors: Tobin Hellyer, James G. Nadeau
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Publication number: 20140134617Abstract: A full process control for use with a molecular assay and a method of determine the efficacy of the molecular assay. A full process control can include a fixed cell, and specifically can include a fixed vegetative cell. A method of determining the efficacy of a molecular assay can include providing an internal control, mixing the internal control with a sample, lysing the internal control and the sample, and detecting the lysis product. The full process control and/or the internal control can be Bacillus subtilis cells.Type: ApplicationFiled: November 7, 2013Publication date: May 15, 2014Applicant: Becton, Dickinson and CompanyInventors: Benoit Dore, Christian Menard, Tobin Hellyer
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Publication number: 20140004502Abstract: Nucleic acid amplification primers and methods for specific detection of influenza A and influenza B nucleic acid targets are disclosed. The primer-target binding sequences are useful for detection of influenza A and influenza B targets in a variety of amplification and hybridization reactions. The oligonucleotide sequences are able to differentiate between influenza A and influenza B strains through specific hybridization to one or the other virus strain, enabling specific detection of the presence of influenza A and/or influenza B in a specimen.Type: ApplicationFiled: June 13, 2013Publication date: January 2, 2014Inventors: Tobin Hellyer, James A. Price, JR., Erika L. Jones
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Patent number: 8597884Abstract: A full process control for use with a molecular assay and a method of determine the efficacy of the molecular assay. A full process control can include a fixed cell, and specifically can include a fixed vegetative cell. A method of determining the efficacy of a molecular assay can include providing an internal control, mixing the internal control with a sample, lysing the internal control and the sample, and detecting the lysis product. The full process control and/or the internal control can be Bacillus subtilis cells.Type: GrantFiled: March 9, 2012Date of Patent: December 3, 2013Assignee: Becton, Dickinson and CompanyInventors: Benoit Dore, Christian Menard, Tobin Hellyer
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Publication number: 20120322067Abstract: Oligonucleotides for detection of nucleic acid in a sample that provide for an amplified signal by recycling probes and probe fragments.Type: ApplicationFiled: November 23, 2010Publication date: December 20, 2012Applicant: BECTON, DICKINSON AND COMPANYInventors: Jim Nadeau, Tobin Hellyer
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Publication number: 20120282605Abstract: A full process control for use with a molecular assay and a method of determine the efficacy of the molecular assay. A full process control can include a fixed cell, and specifically can include a fixed vegetative cell. A method of determining the efficacy of a molecular assay can include providing an internal control, mixing the internal control with a sample, lysing the internal control and the sample, and detecting the lysis product. The full process control and/or the internal control can be Bacillus subtilis cells.Type: ApplicationFiled: March 9, 2012Publication date: November 8, 2012Applicant: GENEOHM SCIENCES, INC.Inventors: Benoit Dore, Christian Menard, Tobin Hellyer
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Publication number: 20110244457Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.Type: ApplicationFiled: March 25, 2011Publication date: October 6, 2011Applicant: Becton, Dickinson and CompanyInventors: James Nadeau, Tobin Hellyer, Dolores M. Berger, William Nussbaumer, Robert Rosenstein, Andrew Kuhn, Sha Sha Wang, Keith Thornton
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Patent number: 7932060Abstract: A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.Type: GrantFiled: April 19, 2004Date of Patent: April 26, 2011Assignee: Becton, Dickinson and CompanyInventors: James Nadeau, Tobin Hellyer, Dolores Berger, William Nussbaumer, Robert Rosenstein, Andrew Kuhn, Sha Sha Wang, Keith Thornton
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Publication number: 20100273156Abstract: Nucleic acid amplification primers and methods for specific detection of influenza A and influenza B nucleic acid targets are disclosed. The primer-target binding sequences are useful for detection of influenza A and influenza B targets in a variety of amplification and hybridization reactions. The oligonucleotide sequences are able to differentiate between influenza A and influenza B strains through specific hybridization to one or the other virus strain, enabling specific detection of the presence of influenza A and/or influenza B in a specimen.Type: ApplicationFiled: May 30, 2008Publication date: October 28, 2010Applicant: BECTON, DICKINSON AND COMPANYInventors: Tobin Hellyer, James A. Price, JR., Erika L. Jones
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Patent number: 7723041Abstract: Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the replicase gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARS-CoV infection.Type: GrantFiled: February 3, 2009Date of Patent: May 25, 2010Assignee: Becton, Dickinson and CompanyInventors: Jianrong Lou, James A. Price, Jr., Daretta A. Yursis, David M. Wolfe, Lisa M. Keller, Tobin Hellyer
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Publication number: 20090258340Abstract: Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the replicase gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARS-CoV infection.Type: ApplicationFiled: February 3, 2009Publication date: October 15, 2009Applicant: Becton, Dickinson and CompanyInventors: Jianrong Lou, James A. Price, JR., Daretta A. Yursis, David M. Wolfe, Lisa M. Keller, Tobin Hellyer
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Publication number: 20090149337Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: ApplicationFiled: April 4, 2008Publication date: June 11, 2009Applicant: Becton, Dickinson and CompanyInventors: Tobin Hellyer, Thomas Fort, Ray A. McMillian, Matthew P. Collis, Thomas L. Fort
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Patent number: 7371531Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: GrantFiled: April 25, 2005Date of Patent: May 13, 2008Assignee: Becton, Dickinson and CompanyInventors: Tobin Hellyer, Thomas Fort, Ray A. McMillian
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Publication number: 20060127924Abstract: The present invention relates, in general, to probes, methods, and kits used to determine the presence or absence of a microorganism in a sample. The probes, methods, and kits comprise at least one capture probe and/or at least one detector probe.Type: ApplicationFiled: August 5, 2005Publication date: June 15, 2006Applicant: Becton, Dickinson and CompanyInventors: Tobin Hellyer, James Nadeau, Jianrong Lou