Patents by Inventor Tokuzo Nishino
Tokuzo Nishino has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7186886Abstract: A protein which has an amino acid sequence shown by SEQ ID NO: 2, 4, 6, 23, 25, 27 or 29, or any of these amino acid sequences modified, and which has activity for transferring an aliphatic acyl group to a glycosyl group at the 5-position of flavonoids, and a gene encoding the same.Type: GrantFiled: June 1, 2001Date of Patent: March 6, 2007Assignee: International Flower Developments Proprietary LimitedInventors: Keiko Sakakibara, Toru Nakayama, Tokuzo Nishino
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Patent number: 6465236Abstract: Bacillus sp. NTAP-1 having been deposited under accession number FERM BP-6926; and a collagen-decomposing enzyme produced by bacterium. The above enzyme (1) has a capability of hydrolyzing, at the highest efficiency, collagen and gelatin from among casein, gelatin, albumin and collagen; (2) shows the optimum pH of 3.5 to 4.5; (3) shows the optimum temperature of 65 to 70° C.; (4) after heating at 60° C. at pH 6.0 for 4 hours, sustains an activity amounting to 60% or more of the level before the heat treatment; (5) remains stable at pH 3 to 6; and a molecular weight of approximately 46,000 when measured by SDS-PAGE.Type: GrantFiled: April 18, 2001Date of Patent: October 15, 2002Assignee: Japan Science and Technology CorporationInventors: Tokuzo Nishino, Toru Nakayama, Naoki Tsuruoka, Minoru Akai
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Patent number: 6413761Abstract: The present invention provides a prenyl diphosphate synthetase and a gene coding for the synthetase. The invention discloses a recombinant protein having the amino acid sequence shown in SEQ ID NO:2 or a recombinant protein which has the amino acid sequence shown in SEQ ID NO:2 having deletion, substitution or addition of at least one amino acid and which has decaprenyl diphosphate synthetase activity; a gene coding for the protein; a recombinant vector comprising the gene; a transformant transformed with the vector; a method for producing a decaprenyl diphosphate synthetase; and a method for producing ubiquinone-10.Type: GrantFiled: March 13, 2001Date of Patent: July 2, 2002Assignee: Toyota Jidosha Kabushiki KaishaInventors: Shusei Obata, Tokuzo Nishino, Tanetoshi Koyama, Yoshihiro Sato
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Patent number: 6410280Abstract: The present invention provides a prenyl diphosphate synthetase and a gene coding for the synthetase. The invention discloses a recombinant protein having the amino acid sequence shown in SEQ ID NO:2 or a recombinant protein which has the amino acid sequence shown in SEQ ID NO:2 having deletion, substitution or addition of at least one amino acid and which has decaprenyl diphosphate synthetase activity; a gene coding for the protein; a recombinant vector comprising the gene; a transformant transformed with the vector; a method for producing a decaprenyl diphosphate synthetase; and a method for producing ubiquinone-10.Type: GrantFiled: March 15, 2001Date of Patent: June 25, 2002Assignee: Toyota Jidosha Kabushiki KaishaInventors: Shusei Obata, Tokuzo Nishino, Tanetoshi Koyama, Yoshihiro Sato
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Patent number: 6395525Abstract: An isolated recombinant protein comprising the amino acid sequence shown in SEQ ID No: 1 is disclosed. A preferred embodiment of the invention is a recombinant protein having the amino acid sequence shown in SEQ ID NO: 1 but having a deletion, substitution or addition of at least one amino acid, excluding the amino acid at position 82, and which has geranyl diphosphate synthase activity. Also disclosed is the gene encoding the recombinant protein.Type: GrantFiled: August 16, 1999Date of Patent: May 28, 2002Assignee: Toyota Jidosha Kabushiki KaishaInventors: Chikara Ohto, Keishi Narita, Tokuzo Nishino, Shin-ichi Ohnuma
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Publication number: 20010051359Abstract: The following recombinant protein (a) or (b) and a gene encoding the same:Type: ApplicationFiled: August 16, 1999Publication date: December 13, 2001Inventors: CHIKARA OHTO, KEISHI NARITA, TOKUZO NISHINO, SHIN-ICHI OHNUMA
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Patent number: 6316216Abstract: A mutant prenyl diphosphate synthase having a modified amino acid sequence of prenyl diphosphate synthase, wherein the amino acid residue located at the eighth position in the N-terminal direction from D of the N-terminal of the Asp-rich domain DDXX(XX)D (in the sequence X denotes any amino acid and the two X's in the parentheses may not be present) present in the region II has been substituted by another amino acid, or both of the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal and the amino acid residue located at the eighth position in the N-terminal direction from D of the N-terminal have been substituted by other amino acids.Type: GrantFiled: April 27, 1999Date of Patent: November 13, 2001Assignee: Toyota Jidosha Kabushiki KaishaInventors: Chikara Ohto, Hiroyuki Nakane, Tokuzo Nishino, Shinichi Ohnuma, Kazutake Hirooka
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Publication number: 20010019838Abstract: The present invention provides a prenyl diphosphate synthetase and a gene coding for the synthetase. The invention discloses a recombinant protein having the amino acid sequence shown in SEQ ID NO:2 or a recombinant protein which has the amino acid sequence shown in SEQ ID NO:2 having deletion, substitution or addition of at least one amino acid and which has decaprenyl diphosphate synthetase activity; a gene coding for the protein; a recombinant vector comprising the gene; a transformant transformed with the vector; a method for producing a decaprenyl diphosphate synthetase; and a method for producing ubiquinone-10.Type: ApplicationFiled: March 13, 2001Publication date: September 6, 2001Inventors: Shusei Obata, Tokuzo Nishino, Tanetoshi Koyama, Yoshihiro Sato
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Patent number: 6225096Abstract: Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.Type: GrantFiled: December 30, 1999Date of Patent: May 1, 2001Assignee: Toyota Jidosha Kabushiki KaishaInventors: Keishi Narita, Chika Ishida, Yoshie Takeuchi, Chikara Ohto, Shinichi Ohnuma, Tokuzo Nishino
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Patent number: 6225097Abstract: The present invention provides a prenyl diphosphate synthetase and a gene coding for the synthetase. The invention discloses a recombinant protein having the amino acid sequence shown in SEQ ID NO:2 or a recombinant protein which has the amino acid sequence shown in SEQ ID NO:2 having deletion, substitution or addition of at least one amino acid and which has decaprenyl diphosphate synthetase activity; a gene coding for the protein; a recombinant vector comprising the gene; a transformant transformed with the vector; a method for producing a decaprenyl diphosphate synthetase; and a method for producing ubiquinone-10.Type: GrantFiled: February 19, 1998Date of Patent: May 1, 2001Assignee: Toyota Jidosha Kabushiki KaishaInventors: Shusei Obata, Tokuzo Nishino, Tanetoshi Koyama, Yoshihiro Sato
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Patent number: 6040165Abstract: Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.Type: GrantFiled: July 1, 1997Date of Patent: March 21, 2000Assignee: Toyota Jidosha Kabushiki KaishaInventors: Keishi Narita, Chika Ishida, Yoshie Takeuchi, Chikara Ohto, Shinichi Ohnuma, Tokuzo Nishino
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Patent number: 5935832Abstract: A mutant prenyl diphosphate synthase capable of synthesizing prenyl diphosphates, shorter than those synthesized by the original enzyme, by modifying the amino acid sequence in and upstream of the aspartic acid-rich domain DDXX(XX)D (X denotes any amino acid, and XX in the parentheses may not be present) present in region II of the prenyl diphosphate synthase.Type: GrantFiled: July 22, 1997Date of Patent: August 10, 1999Assignee: Toyota Jidosha Kabushiki KaishaInventors: Hiroyuki Nakane, Chikara Ohto, Shinichi Ohnuma, Kazutake Hirooka, Tokuzo Nishino
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Patent number: 5885810Abstract: The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.Type: GrantFiled: April 1, 1998Date of Patent: March 23, 1999Assignee: Toyota Jidosha Kabushiki KaishaInventors: Chikara Ohto, Chika Asada, Shinichi Ohnuma, Tokuzo Nishino, Kazutake Hirooka, Hisashi Hemmi
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Patent number: 5882909Abstract: The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein, at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.Type: GrantFiled: April 1, 1998Date of Patent: March 16, 1999Assignee: Toyota Jidosha Kabushiki KaishaInventors: Chikara Ohto, Chika Asada, Shinichi Ohnuma, Tokuzo Nishino, Kazutake Hirooka, Hisashi Hemmi
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Patent number: 5807725Abstract: The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein, at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.Type: GrantFiled: August 29, 1996Date of Patent: September 15, 1998Assignee: Toyota Jidosha Kabushiki KaishaInventors: Chikara Ohto, Chika Asada, Shinichi Ohnuma, Tokuzo Nishino, Kazutake Hirooka, Hisashi Hemmi
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Patent number: 5773273Abstract: DNA coding for thermostable geranylgeranyl diphosphate (GGDP) synthase derived from Sulfolobus acidocaldarius is provided. The DNA is useful for production of GGDP synthase, which is, in turn, useful for production of GGDP.Type: GrantFiled: March 24, 1995Date of Patent: June 30, 1998Assignee: Toyota Jidosha Kabushiki KaishaInventors: Tokuzo Nishino, Shinichi Ohnuma, Manabu Suzuki, Chikara Ohto, Chika Asada, Yuka Higuchi, Yoshie Takeuchi
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Patent number: 5766911Abstract: A mutated farnesyldiphosphate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding for said mutated enzyme, wherein the mutated enzyme is modified from a native farnesyldiphosphate synthase by mutation of a gene coding for a native farnesyldiphosphate synthase.Type: GrantFiled: September 28, 1995Date of Patent: June 16, 1998Assignee: Toyota Jidosha Kabushiki KaishaInventors: Ayumi Koike, Shusei Obata, Tokuzo Nishino, Shinichi Ohnuma, Takeshi Nakazawa, Kyozo Ogura, Tanetoshi Koyama
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Patent number: RE43425Abstract: A mutant prenyl diphosphate synthase capable of synthesizing prenyl diphosphates, shorter than those synthesized by the original enzyme, by modifying the amino acid sequence in and upstream of the aspartic acid-rich domain DDXX (XX)D (X denotes any amino acid, and XX in the parentheses may not be present) present in region II of the prenyl diphosphate synthase.Type: GrantFiled: July 12, 2001Date of Patent: May 29, 2012Assignee: Toyota Jidosha Kabushiki KaishaInventors: Hiroyuki Nakane, Chikara Ohto, Shinichi Ohnuma, Kazutake Hirooka, Tokuzo Nishino