Abstract: A coupling member is disclosed for use in connecting together flexible tubes and the like. The coupling member is adapted for connection with a mating coupling member, and includes an outer rigid coupling component which is thermally resistant and an inner coupling component which is thin and flexible, so that the inner coupling component can provide a penetrable seal and whereby the overall coupling member is capable of withstanding autoclaving temperatures so that it can be sterilized prior to use. A coupling device including such a coupling member is also disclosed, including a second coupling member which is capable of mating with the first coupling member and which includes a penetrating portion so that when the two coupling members are mated, the penetrating portion of the second coupling member penetrates the inner coupling component of the first coupling member.