Abstract: The object of the invention is the method of detection and selection of hybridoma cells capable to produce desired antibodies, comprising seeding the hybridoma cells in a culture vessel with biofunctionalized surface, containing culture medium, adding biofunctionalized luminescent labels and incubating such hybridoma cell culture, followed by optical detection of hybridoma cells producing desired antibodies by reaction of he biofunctionalized luminescent labels with the antibodies and detecting a luminescent label's signal border around hybridoma cells producing desired antibodies, and further separation in situ hybridoma cells producing the given antibody type from the rest of the cells.

Abstract: A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.

Abstract: The subject the present invention is a method of producing a pharmacologically stable form of purified and lyophilised bacteriophage preparations of increased stability and antibacterial activity, characterised in that phages produced from a bacterial lysate, for example by ultrafiltration on ultrafiltration membranes, containing a high molecular weight preparation of purified phages is stabilised most preferably with a probiotic extract in the presence or absence of neutral salts and/or organic solvents, lyophilised, characterised via HPLC chromatography, by SDS-PAGE, and bacterial lysis biological assays, and then stored under a vacuum, where the active lyophilisate is destined for phage therapy of infections and tumours.

Abstract: The subject the present invention is a method of producing affinity beds with bacterial lipopolysaccharide molecules bound to them in a non-covalent and stable fashion. The bed produced according to the present invention may be used in affinity chromatography for the purification of antibodies against lipopolysaccharides. It is particularly useful in the purification of antibodies against the labile epitopes of lipopolysaccharides, which degrade in heretofore used methods of covalent bonding of the lipopolysaccharide molecules to the solid phase.

Abstract: A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.

Abstract: The present invention provides immunogenic oligosaccharide compositions and methods of making and using them. In particular, the compositions comprise native O-linked and S-linked oligosaccharides coupled to a protein carrier via a linker, wherein the resultant conjugate elicits a protectively immunogenic response, particularly in vaccines against pathogenic Candida species and more particularly against Candida albicans. Preferably the pathogenic Candida species are those that possess cell wall oligosaccharide compositions similar to the ?-mannan component of Candida albicans cell walls.