Abstract: An object of the present invention is to provide an enzyme preparation useful for reducing purine bodies, especially, in beer or beer-based beverages, and use thereof. Provided is a nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less.

Abstract: It is an object of the present invention to provide a novel enzyme useful for producing low-purine foods or beverages. There is disclosed a nucleosidase comprising an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.

Abstract: An object of the present invention is to provide an enzyme preparation useful for reducing purine bodies, especially, in beer or beer-based beverages, and use thereof. Provided is a nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less.

Abstract: It is an object of the present invention to provide a novel enzyme useful for producing low-purine foods or beverages. There is disclosed a nucleosidase comprising an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.

Abstract: Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.

Abstract: Provided herein are a method for selecting a gene participating in the desire brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.

Abstract: Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.

Abstract: Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.

Abstract: An object of the present invention is to provide a method for analyzing gene of an industrial yeast. The method of the present invention comprises (a) analyzing the genome sequence of the industrial yeast; and (c-1) selecting a gene of the industrial yeast encoding an amino acid sequence having 70 to 97% identity to an amino acid sequence encoded by the gene of Saccharomyces cerevisiae, or (c-2) selecting a gene of the industrial yeast consisting of a nucleotide sequence having 60 to 94% identity to the nucleotide sequence of the gene of Saccharomyces cerevisiae.

Abstract: An object of the present invention is to provide a method for analyzing gene of an industrial yeast. The method of the present invention comprises (a) determining the whole genome sequence of the industrial yeast; and (c-1) selecting a gene of the industrial yeast encoding an amino acid sequence having 70 to 97% identity to an amino acid sequence encoded by the gene of Saccharomyces cerevisiae, or (c-2) selecting a gene of the industrial yeast consisting of a nucleotide sequence having 60 to 94% identity to the nucleotide sequence of the gene of Saccharomyces cerevisiae.

Abstract: The present invention provides (A) a method for the selection of genes participating in the desired brewing character, which comprises preparing a database compiling the data of the whole genome sequence of industrial yeast, particularly a brewing yeast used for alcoholic beverages; selecting gene participating in a desired brewing character that the brewing yeast specifically possesses; and carrying out functional analysis of the gene by disruption or overexpression; (B) a DNA array in which oligonucleotide(s) selected based on the data base compiling the data of the whole genome sequences of an industrial yeast, (C) a breeding method for constructing improved cultures achieving the desired brewing character, (D) a method for producing an alcohol or an alcoholic beverage in which productivity and quality are improved using the yeast, (E) a gene which is specific to the improved brewing yeast, and (F) a peptide encoded by the gene.