Patents by Inventor Tomoko Kunitomi
Tomoko Kunitomi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230323310Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.Type: ApplicationFiled: September 3, 2021Publication date: October 12, 2023Applicants: TOPPAN INC., OSAKA UNIVERSITYInventors: Tomoko KUNITOMI, Shiro KITANO, Kei TSUKAMOTO, Michiya MATSUSAKI
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Patent number: 11739288Abstract: A method for filling an analytical device includes providing a plate having a detection space or spaces and ports communicating with the space(s), fitting, into one of the ports, an adapter having a reagent fixed on an inner wall of the adapter such that a first end of the adapter is fitted into the port, and delivering a sample solution into the detection space(s) through the adapter such that the sample solution and reagent fill the detection space(s). The plate includes a first substrate, a second substrate, and a side wall such that the plate has the detection space(s) between the substrates and that the first substrate has the ports, the first and/or second substrates is formed of transparent material such that the detection space is observable from outside the plate, and the ports include a first port for delivering liquid-containing substance, and a second port for discharging the substance.Type: GrantFiled: September 22, 2021Date of Patent: August 29, 2023Assignee: TOPPAN PRINTING CO. , LTD.Inventors: Tomoko Kunitomi, Yoichi Makino
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Publication number: 20230203452Abstract: A method of producing a conditioned medium for culturing a patient-derived cancer cell, including culturing, in a first medium for 24 hours or longer, a three-dimensional cell tissue including an extracellular matrix component, a polymer electrolyte, and a cell cluster including a fibroblast, and collecting the first medium in which the three-dimensional cell tissue has been cultured as the conditioned medium.Type: ApplicationFiled: February 21, 2023Publication date: June 29, 2023Applicant: TOPPAN Inc.Inventors: Kei TSUKAMOTO, Shiro KITANO, Tomoko KUNITOMI
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Publication number: 20230117082Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that theType: ApplicationFiled: December 16, 2022Publication date: April 20, 2023Applicant: TOPPAN Inc.Inventors: Yoichi MAKINO, Akihiro HOSHINO, Tomoko KUNITOMI, Keisuke GOTO, Yosuke HORIUCHI
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Patent number: 11559804Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that theType: GrantFiled: October 29, 2018Date of Patent: January 24, 2023Assignee: TOPPAN PRINTING CO., LTD.Inventors: Yoichi Makino, Akihiro Hoshino, Tomoko Kunitomi, Keisuke Goto, Yosuke Horiuchi
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Publication number: 20220290222Abstract: A biomolecule analysis method may include preparing a reaction container comprising an array of a plurality of micropores in which a wash buffer is filled or preparing the reaction container and feeding the wash buffer into the reaction container including the array of the plurality of micropores. The method may include feeding a reaction reagent to detect a sample, into the plurality of micropores, the sample including a biomolecule. The method may include feeding a sealing solution to seal the plurality of micropores so that the plurality of micropores become a plurality of independent reaction chambers sealed from each other. The method may include causing an isothermal detection reaction in each of the plurality of the independent reaction containers. The method may include detecting a signal amplified by the isothermal detection reaction.Type: ApplicationFiled: May 23, 2022Publication date: September 15, 2022Applicant: TOPPAN PRINTING CO., LTD.Inventors: Yoichi MAKINO, Tomoko KUNITOMI
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Patent number: 11371089Abstract: A biomolecule analysis method including: feeding a reagent into a reaction container which includes a plurality of wells and filing the reagent in the plurality of wells, the reagent being for causing an enzymatic reaction with regard to a target substance of analysis; feeding an oil sealing solution over the plurality of wells and sealing the reagent into the plurality of wells with the oil sealing solution so that the plurality of wells become a plurality of independent reaction containers; performing the enzymatic reaction by incubating the reaction container; and detecting a signal amplified by the enzymatic reaction.Type: GrantFiled: April 17, 2019Date of Patent: June 28, 2022Assignee: TOPPAN PRINTING CO., LTD.Inventors: Yoichi Makino, Tomoko Kunitomi
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Patent number: 11261484Abstract: A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.Type: GrantFiled: July 27, 2016Date of Patent: March 1, 2022Assignee: TOPPAN PRINTING CO., LTD.Inventors: Yoichi Makino, Tomoko Kunitomi
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Publication number: 20220002645Abstract: A method for filling an analytical device includes providing a plate having a detection space or spaces and ports communicating with the space(s), fitting, into one of the ports, an adapter having a reagent fixed on an inner wall of the adapter such that a first end of the adapter is fitted into the port, and delivering a sample solution into the detection space(s) through the adapter such that the sample solution and reagent fill the detection space(s). The plate includes a first substrate, a second substrate, and a side wall such that the plate has the detection space(s) between the substrates and that the first substrate has the ports, the first and/or second substrates is formed of transparent material such that the detection space is observable from outside the plate, and the ports include a first port for delivering liquid-containing substance, and a second port for discharging the substance.Type: ApplicationFiled: September 22, 2021Publication date: January 6, 2022Applicant: TOPPAN PRINTING CO., LTD.Inventors: Tomoko KUNITOMI, Yoichi MAKINO
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Patent number: 11142731Abstract: An analytical device includes a plate including a first substrate having a front surface and a back surface and a second substrate facing the back surface of the first substrate such that at least one detection space observable from outside is formed between the second substrate and the back surface of the first substrate, and an adapter. The first substrate has a first port having a through hole formed through the front surface and communicated with the detection space for delivering a liquid-containing substance into the detection space, the first substrate has a second port having a through hole formed through the front surface and communicated with the detection space for discharging a liquid-containing substance or a gas from the detection space, and the adapter is attachable to and detachable from the first port and the second port, and the front surface of the first substrate without the adapter is flat.Type: GrantFiled: December 26, 2017Date of Patent: October 12, 2021Assignee: TOPPAN PRINTING CO., LTD.Inventors: Tomoko Kunitomi, Yoichi Makino
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Patent number: 10894973Abstract: An array device including a base having wells, a cover positioned over the base with a gap from the base such that openings of the wells are covered by the cover with the gap in between, an injection port communicating with the gap, a discharge port communicating with the gap and positioned apart from the injection port, and a waste liquid vessel which collects liquid that flows from the gap via the discharge port and is positioned at a level different from the gap forming a channel. The discharge port is placed to discharge a surplus aqueous solution outside the wells from the discharge port by an oleaginous sealing liquid to be delivered from the injection port.Type: GrantFiled: May 4, 2017Date of Patent: January 19, 2021Assignee: TOPPAN PRINTING CO., LTD.Inventors: Yoichi Makino, Tomoyuki Ozawa, Shuichi Akashi, Tomoko Kunitomi
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Publication number: 20200003691Abstract: A device including a well array including wells in which a target substance is detected based on a biochemical reaction thereof. Each of the wells has an aspect ratio of 1 or more as calculated by Formula (1) such that the biochemical reaction of the target substance is detected, Aspect ratio=Depth of each well/Diameter of largest circle among circles included in a region enclosed by a rim of each well??Formula (1).Type: ApplicationFiled: September 13, 2019Publication date: January 2, 2020Applicant: TOPPAN PRINTING CO., LTD.Inventors: Misaki NAGAHARA, Yoichi MAKINO, Akihiro HOSHINO, Takumi HIRASE, Tomoko KUNITOMI, Keisuke GOTO, Yusuke HORIUCHI
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Publication number: 20190241948Abstract: A biomolecule analysis method including: feeding a reagent into a reaction container which includes a plurality of wells and filing the reagent in the plurality of wells, the reagent being for causing an enzymatic reaction with regard to a target substance of analysis; feeding an oil sealing solution over the plurality of wells and sealing the reagent into the plurality of wells with the oil sealing solution so that the plurality of wells become a plurality of independent reaction containers; performing the enzymatic reaction by incubating the reaction container; and detecting a signal amplified by the enzymatic reaction.Type: ApplicationFiled: April 17, 2019Publication date: August 8, 2019Applicant: TOPPAN PRINTING CO., LTD.Inventors: Yoichi Makino, Tomoko Kunitomi
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Publication number: 20190060897Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that theType: ApplicationFiled: October 29, 2018Publication date: February 28, 2019Applicant: TOPPAN PRINTING CO., LTD.Inventors: Yoichi MAKINO, Akihiro HOSHINO, Tomoko KUNITOMI, Keisuke GOTO, Yosuke HORIUCHI
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Publication number: 20180119078Abstract: An analytical device includes a plate including a first substrate having a front surface and a back surface and a second substrate facing the back surface of the first substrate such that at least one detection space observable from outside is formed between the second substrate and the back surface of the first substrate, and an adapter. The first substrate has a first port having a through hole formed through the front surface and communicated with the detection space for delivering a liquid-containing substance into the detection space, the first substrate has a second port having a through hole formed through the front surface and communicated with the detection space for discharging a liquid-containing substance or a gas from the detection space, and the adapter is attachable to and detachable from the first port and the second port, and the front surface of the first substrate without the adapter is flat.Type: ApplicationFiled: December 26, 2017Publication date: May 3, 2018Applicant: TOPPAN PRINTING CO., LTD.Inventors: Tomoko KUNITOMI, Yoichi MAKINO
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Publication number: 20170233790Abstract: An array device including a base having wells, a cover positioned over the base with a gap from the base such that openings of the wells are covered by the cover with the gap in between, an injection port communicating with the gap, a discharge port communicating with the gap and positioned apart from the injection port, and a waste liquid vessel which collects liquid that flows from the gap via the discharge port and is positioned at a level different from the gap forming a channel. The discharge port is placed to discharge a surplus aqueous solution outside the wells from the discharge port by an oleaginous sealing liquid to be delivered from the injection port.Type: ApplicationFiled: May 4, 2017Publication date: August 17, 2017Applicant: TOPPAN PRINTING CO., LTD.Inventors: Yoichi Makino, Tomoyuki Ozawa, Shuichi Akashi, Tomoko kunitomi
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Publication number: 20160333400Abstract: A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.Type: ApplicationFiled: July 27, 2016Publication date: November 17, 2016Applicant: TOPPAN PRINTING CO. LTD.Inventors: Yoichi MAKINO, Tomoko Kunitomi