Abstract: Disclosed is a measurement apparatus for analyzing a cell contained in a specimen, comprising: a chamber for preparing a measurement sample in which the cell is stained with first and second fluorescent dyes contained in a reagent supplied from at least one reagent container; a liquid feeding section for feeding the reagent from the reagent container to the chamber via a liquid feeding tube provided between the reagent container and the chamber; and a detection section that acquires first and second signals each corresponding to fluorescence of a first wavelength and fluorescence of a second wavelength emitted from the cell stained with the first and second fluorescent dyes in response to irradiation of the measurement sample flowing in a flow cell with light; and an analysis section that analyzes the cell on the basis of the first and second signals.

Abstract: The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The present invention also provides a regent kit and reagent for classifying leukocyte which are used for classifying and counting leukocytes in biological samples.

Abstract: Disclosed is a method of detecting filarial larvae in blood. The method comprises preparing a measurement sample from a blood sample collected from an animal; flowing the prepared measurement sample through a flow cell; irradiating light on the measurement sample flowing through the flow cell; detecting light given off from the irradiated measurement sample; and detecting filarial larvae contained in the measurement sample based on characteristic parameter of the detected light.

Abstract: Disclosed is a method of detecting filarial larvae in blood. The method comprises preparing a measurement sample from a blood sample collected from an animal; flowing the prepared measurement sample through a flow cell; irradiating light on the measurement sample flowing through the flow cell; detecting light given off from the irradiated measurement sample; and detecting filarial larvae contained in the measurement sample based on characteristic parameter of the detected light.

Abstract: The present invention provides a reagent kit for analyzing a sample comprising a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid and a second reagent containing a fluorescent dye capable of staining nucleic acid, and a method for analyzing a sample using the kit.

Abstract: The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The present invention also provides a regent kit and reagent for classifying leukocyte which are used for classifying and counting leukocytes in biological samples.

Abstract: A reagent for measuring basophils and/or nucleated red blood cells in a sample, which comprises at least one fluorescent dye defined in the formula (I) or (II) is disclosed. Also disclosed are a kit and method for measuring basophils and/or nucleated red blood cells.

Abstract: A reagent for measuring a basophil and/or a nucleated red blood cell is described. The reagent comprises (a) an alcohol having 4 to 8 total carbon atoms, and (b) one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I) and a compound of the general formula (II).

Abstract: A method for classifying and counting leukocytes, which comprises steps of: staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent, with a fluorescent dye; introducing the sample containing the stained cells into a flow cytometer to measure first scattered light, second scattered light different from the first scattered light and fluorescence of the respective cells; obtaining scattered light peak intensities and scattered light widths of the respective cells based on the measured first scattered light, obtaining scattered light intensities of the respective cells based on the measured second scattered light, and obtaining fluorescence intensities of the respective cells based on the measured fluorescence light; classifying the cells into a first group and a second group based on the scattered light peak intensities and the scattered light widths, the first group including leukocytes and second group including platelet clumps; classifying the leukocytes included in

Abstract: The present invention provides a reagent kit for analyzing a sample comprising a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid and a second reagent containing a fluorescent dye capable of staining nucleic acid, and a method for analyzing a sample using the kit.

Abstract: There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the

Abstract: A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.

Abstract: Methods and devices are described for monitoring a therapeutic effect in a red blood cell-related disease by detecting the size of a reticulocyte using a full-automatic blood cell counting device.

Abstract: 1.

Abstract: A reagent for measuring a basophil and/or a nucleated red blood cell is described. The reagent comprises (a) an alcohol having 4 to 8 total carbon atoms, and (b) one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I) and a compound of the general formula (II).

Abstract: A reagent for measuring basophils and/or nucleated red blood cells in a sample, which comprises at least one fluorescent dye defined in the formula (I) or (II) is disclosed. Also disclosed are a kit and method for measuring basophils and/or nucleated red blood cells.

Abstract: A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.

Abstract: There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the ste

Abstract: Methods and devices are described for monitoring a therapeutic effect in a red blood cell-related disease by detecting the size of a reticulocyte using a full-automatic blood cell counting device.

Abstract: A method for evaluating an artificial receptor includes subjecting a functional monomer and a crosslinker in the presence of a template substance in a vessel into polymerization, thereby forming a resultant polymer with the template substance usually in the form of a thin film, dissociating the template substance from the polymer and removing it from the vessel by washing an inside of the vessel, thereby to prepare an artificial receptor, feeding a solution of the same or different template substance into the vessel to associate the template substance with the resulting polymer; then determining the amount of the template substance remaining in the vessel, and evaluating the artificial receptor on the basis of a determination result.