Abstract: A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3? end portion, a sequence (Ac?) that hybridizes to a sequence (A) located in the 3? end portion of the target nucleic acid sequence. The first primer also contains, on the 5? side of the sequence (Ac?), a sequence (B?) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5? side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3? end portion, a sequence (Cc?) that hybridizes to a sequence (C) located in the 3? end portion of a complementary sequence to the target nucleic acid sequence.

Abstract: A method of analyzing a biosample that enables substantial shortening of time required for analysis, further enabling obtaining highly reliable results through means for avoiding sway of analytical results depending on observers, and that enables one-time analysis of a multiplicity of genes, etc. on a single biosample to thereby enhance workload and time efficiencies, and that enables analysis of multiple genes, etc. under conditions completely free from any difference in background attributed to biosamples. There is provided a method comprising irradiating a biosample as an analyte with ultra-short pulse laser beams to thereby effect an ablation thereof so that molecules contained in the biosample are atomized into constituting elements, ionizing the constituting elements resulting from the atomization and analyzing the ionized constituting elements to thereby analyze analytical-target molecules of the biosample.

Abstract: A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3? end portion, a sequence (Ac?) that hybridizes to a sequence (A) located in the 3? end portion of the target nucleic acid sequence. The first primer also contains, on the 5? side of the sequence (Ac?), a sequence (B?) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5? side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3? end portion, a sequence (Cc?) that hybridizes to a sequence (C) located in the 3? end portion of a complementary sequence to the target nucleic acid sequence.

Abstract: The invention is a method and a kit for preparing single-stranded DNA from double-stranded DNA and the purification of single-stranded DNA derived from double-stranded DNA. A single-stranded-DNA binding substance is used in combination with a double-strand-specific endonuclease for the removal of undesired double-stranded DNA from a single-stranded DNA preparation and for other related purposes.

Abstract: A testing chip for detecting a target nucleic acid from a liquid sample which at least comprises a support, a sample introduction part provided on the support, and a test region wherein an amplification reagent for amplifying the target nucleic acid is immobilized in the inside or on the surface of the support.

Abstract: The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for detecting a target nucleic acid from a sample, comprising at least a first compartment which contains an extraction reagent composition for extracting nucleic acids from said sample, a second compartment which contains an amplification reagent composition for amplifying the target nucleic acid, a separating means for separating the first and second compartments, and an aperture which enables to introduce said sample into only said first compartment.

Abstract: The invention provides a sequence specific method for amplifying nucleic acids. More particularly, the invention provides a method for amplifying nucleic acid sequences which enables such sequences to be detected with high precision, rapidity and high specificity as compared to conventional methods. The present invention also provides a simple method for cloning nucleic acids, particularly, a rapid and simple method for amplifying alternative splicing forms synthesized by an alternative splicing which is performed in a process of preparing a matured mRNA from a DNA.