Abstract: A collection device is disclosed having a tubular housing detachably receiving a valved bulb reservoir at a first end thereof, the housing having a second end for dispensing fluids. The valved bulb reservoir has a sample capillary tube attached at one end thereof. The bore of the capillary is fluidically coupled to the valved bulb reservoir. The capillary is removed from the tubular housing to collect a sample in its bore, and is then replaced into the tubular housing. The contents of the valved tubular reservoir, which can be a buffer solution, a reagent, or other analytic fluid, or a gas, are then passed through the capillary bore to expel the sample and mix therewith in the housing. The sample product can then be dispensed from the tubular housing.

Abstract: A collection device is disclosed having a tubular housing detachably receiving a valved bulb reservoir at a first end thereof, the housing having a second end for dispensing fluids. The valved bulb reservoir has a sample capillary tube attached at one end thereof. The bore of the capillary is fluidically coupled to the valved bulb reservoir. The capillary is removed from the tubular housing to collect a sample in its bore, and is then replaced into the tubular housing. The contents of the valved tubular reservoir, which can be a buffer solution, a reagent, or other analytic fluid, or a gas, are then passed through the capillary bore to expel the sample and mix therewith in the housing. The sample product can then be dispensed from the tubular housing.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: A collection device is disclosed having a tubular housing detachably receiving a valved bulb reservoir at a first end thereof, the housing having a second end for dispensing fluids. The valved bulb reservoir has a sample capillary tube attached at one end thereof. The bore of the capillary is fluidically coupled to the valved bulb reservoir. The capillary is removed from the tubular housing to collect a sample in its bore, and is then replaced into the tubular housing. The contents of the valved tubular reservoir, which can be a buffer solution, a reagent, or other analytic fluid, or a gas, are then passed through the capillary bore to expel the sample and mix therewith in the housing. The sample product can then be dispensed from the tubular housing.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.