Abstract: The present inventors aimed to identify the gene responsible for the temperature-sensitive male sterility trait in “PL12” and to provide a method for producing conditional male sterile plants targeting the gene, and performed whole genome sequence analysis on “PL12” and the original variety thereof to compare the two. As a result, it was found that there was a deletion of about 150 kb in “PL12” chromosome 7. Then, as for this region, six types of partial deletion lines were produced by genome editing, and the presence or absence of the temperature-sensitive male sterility trait in each line was used as an index to successfully narrow the range, containing the responsible mutation, down to about 10 kb. Furthermore, as a result of creating a line with genome editing-disrupted functions of the two genes located in this region, the gene responsible for the temperature-sensitive male sterility trait was successfully identified.

Abstract: This invention is intended to develop many DNA markers in plants of the genus Fragaria and to evaluate anthracnose resistance with high accuracy with the use of such many DNA markers. The markers associated with anthracnose resistance in plants of the genus Fragaria each comprise a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 10 in the chromosome of a plant of the genus Fragaria.

Abstract: The present invention provides a parthenocarpy regulatory gene including any of the following polynucleotides and use of the parthenocarpy regulatory gene. (1) A polynucleotide that encodes an amino acid sequence represented by SEQ ID NO: 1, (2) a polynucleotide that encodes an amino acid sequence having a sequence identity of 70% or higher relative to the amino acid sequence represented by SEQ ID NO: 1, (3) a polynucleotide that encodes an amino acid sequence in which 1 to 87 amino acids are substituted and/or the like in the amino acid sequence represented by SEQ ID NO: 1, and (4) a polynucleotide that is hybridized with a polynucleotide having a sequence complementary to the polynucleotide described in the above (1) under a stringent condition.

Abstract: This invention is intended to develop many DNA markers in plants of the genus Fragaria and to evaluate anthracnose resistance with high accuracy with the use of such many DNA markers. The markers associated with anthracnose resistance in plants of the genus Fragaria each comprise a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 10 in the chromosome of a plant of the genus Fragaria.