Abstract: A method for classifying data executed by a computer includes obtaining a plurality of data groups, each of the data groups including a plurality of data items about detected intensities being associated with physical index values, respectively; and classifying, based on identification information of each of the data groups and the physical index values, the data items included in the data groups into a plurality of clusters.

Abstract: A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.

Abstract: A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.

Abstract: A novel ion trap made of at least two ion guides sets separated by a gap and each guide consists of three or more rods-like multipole carrying radio frequency (RF) voltages with delayed phases. The injected ions are axially or orthogonally, contained by pulsed DC and/or RF voltages. When the ions translational energy is damped due to collisions with a low-pressurized inert gas, the 3-D RF field in the gap, which is created by the special rod and electricity arrangement, can trap the ions and compact them in a dense ion cloud. Because the ions are trapped in the small gap, new ions can be injected and the trapping cycle can be repeated many times before the ion ejection. The ions are ejected from the gap orthogonally or axially. This ion trap is useful for mass spectrometry and beam physics, specifically for high efficient ion accumulation and focusing the ions in a small space.

Abstract: The invention provide the method to enable the molecular detections of cellular fluid of at least a single cell easily and rapidly and clarify the dynamics of life phenomena and molecular mechanisms very rapidly by conducting simultaneous observations of cellular morphological dynamism. At the same time, it provides the method of exploring active molecules. Even in case that the target is a single cell, the cellular fluid is directly sucked by the capillary tip and the fluid is subjected to the analysis directly. By performing this method under the observation of the cell with image-magnifying scopes, such as a microscope, the cellular fluid is sucked and captured by the nanospray ionization capillary tip even if the target is a single cell and the trapped sample is subjected to the mass spectrometer by nano-spraying the sample solution directly after addition of the ionization supporting solvent into the tip, and then the molecular components in the cellular fluid is detected.