Abstract: The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention.

Abstract: The present invention relates to a population of cells comprising hepatic stem-like cells and therapeutic use thereof, for the treatment and the prevention of fulminant liver disorders. The hepatic stem-like cells according to the invention may be safely and reproducibly generated from pluripotent stem cells. In addition, although the hepatic stem-like cells according to the invention do not display the phenotype of physiologically mature hepatic cells, as they are lacking the albumin expression marker (ALB?), they may still be transplanted in a diseased liver with acute failure, rescue the diseased liver and promote liver regeneration. Moreover, various protocols of preparation of hepatic stem-like cells according to the invention may be implemented, all resulting in high quality and high yield of production. Finally, the hepatic stem-like cells according to the invention may be cryopreserved and may also be prepared as spheroid particles.

Abstract: Aspects of the present disclosure may include a vehicle having one or more tires, a body, and an occupant compartment including: a rotatable platform, a central pod seat and one or more companion pod seats disposed on the rotatable platform, and one or more rear seats, wherein the rotatable platform is configured to rotate the central pod seat and the one or more companion pod seats toward a front of the vehicle in a first mode and toward a rear of the vehicle in a second mode, and wherein the central pod seat is further configured to translate linearly away from a center of the rotatable platform into a first position and toward the center of the rotatable platform into a second position.

Abstract: Aspects of the present disclosure may include a vehicle having one or more tires, a body, and an occupant compartment including: a rotatable platform, a central pod seat and one or more companion pod seats disposed on the rotatable platform, and one or more rear seats, wherein the rotatable platform is configured to rotate the central pod seat and the one or more companion pod seats toward a front of the vehicle in a first mode and toward a rear of the vehicle in a second mode, and wherein the central pod seat is further configured to translate linearly away from a center of the rotatable platform into a first position and toward the center of the rotatable platform into a second position.

Abstract: The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention.