Abstract: Disclosed herein is mesoscale bioreactor platform comprising an upwards open chamber for a biological cell, which chamber via a first port is in communication with a first channel for conducting an influent stream of a liquid into the chamber and via a second port is in communication with a second channel for conducting an effluent stream of a liquid away from the chamber, which chamber is provided with a closure comprising a water-immiscible liquid, and wherein said first channel is in fluid communication with a reservoir for a liquid and said second channel is in fluid communication with a waste container. Furthermore, a method for modifying the interaction of a content of a chamber with the surroundings is described as well as method of culturing a biological cell.

Abstract: Disclosed is a mesoscale bioreactor platform including two or more liquid reservoirs in fluid communication with a culture chamber which chamber is in fluid communication with an exit. The platform allows the chamber to be perfused with a flow of liquid from one or more of the liquid reservoirs. The integrated reservoirs for liquids remove the need for external supplies of liquid to the culture chamber during cell culture experiments requiring perfusion of liquids. Moreover, with two or more reservoir it is possible to supply the culture chamber with different types of liquids.

Abstract: The invention relates to methods and devices for forming a concentration gradient of a reagent for use in chemotactic evaluation. A flow passage is provided and defined at least in part by a substrate having a target region on a surface thereof. A concentration gradient of a reagent is formed over the target region by controlled delivery of a fluid containing the reagent in laminar flow through the flow passage and over the target region. The concentration gradient is suitable for chemotactic evaluation. Typically, the inventive methods and devices are employed to evaluate the chemotactic interaction between a candidate compound and a monolayer of immobilized cells.