Patents by Inventor Umberto Ulmanella
Umberto Ulmanella has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9127306Abstract: Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.Type: GrantFiled: February 17, 2009Date of Patent: September 8, 2015Assignee: Life Technologies CorporationInventors: Vladimir I. Bashkirov, Umberto Ulmanella, Robert G. Eason, Bradford J. Taft
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Publication number: 20150218630Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures.Type: ApplicationFiled: December 2, 2014Publication date: August 6, 2015Inventors: Hongye SUN, Mark F. Oldham, John O'Neill, Charles R. Connell, Umberto Ulmanella, Aldrich N.K. Lau, Theo Kotseroglou, Kenneth J. Livak
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Patent number: 9011663Abstract: The present teachings relate to microfluidic valves and pumping systems, which may be suitable for controlling and facilitating liquid flow. Electrodes are disposed proximately to volumes containing a liquid. The liquid flow can be facilitated by electrowetting forces. Processes for controlling the flow of liquids, as well as for pumping liquids, are also disclosed.Type: GrantFiled: September 16, 2013Date of Patent: April 21, 2015Assignee: Applied Biosystems, LLCInventor: Umberto Ulmanella
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Patent number: 8969090Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures.Type: GrantFiled: January 4, 2011Date of Patent: March 3, 2015Assignee: Life Technologies CorporationInventors: Hongye Sun, Eric S. Nordman, Mark F. Oldham, John R. O'Neill, Charles Connell, Umberto Ulmanella, Aldrich N. K. Lau, Theofilos Kotseroglou, Kenneth J. Livak
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Publication number: 20140162264Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.Type: ApplicationFiled: December 2, 2013Publication date: June 12, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
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Publication number: 20140076436Abstract: The present teachings relate to microfluidic valves and pumping systems, which may be suitable for controlling and facilitating liquid flow. Electrodes are disposed proximately to volumes containing a liquid. The liquid flow can be facilitated by electrowetting forces. Processes for controlling the flow of liquids, as well as for pumping liquids, are also disclosed.Type: ApplicationFiled: September 16, 2013Publication date: March 20, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventor: Umberto Ulmanella
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Patent number: 8597590Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.Type: GrantFiled: May 27, 2010Date of Patent: December 3, 2013Assignee: Applied Biosystems, LLCInventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
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Patent number: 8383062Abstract: A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.Type: GrantFiled: November 12, 2010Date of Patent: February 26, 2013Assignee: Applied Biosystems, LLCInventors: Umberto Ulmanella, Debjyoti Banerjee, Konrad Faulstich, Aldrich Lau, Jun Xie
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Publication number: 20120214156Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.Type: ApplicationFiled: February 23, 2012Publication date: August 23, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Debjyoti BANERJEE, Konrad Faulstich, Aldrich N.K. Lau, Umberto Ulmanella, Jun Xie
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Publication number: 20120138158Abstract: The present teachings relate to microfluidic valves and pumping systems, which may be suitable for controlling and facilitating liquid flow. Electrodes are disposed proximately to volumes containing a liquid. The liquid flow can be facilitated by electrowetting forces. Processes for controlling the flow of liquids, as well as for pumping liquids, are also disclosed.Type: ApplicationFiled: December 6, 2011Publication date: June 7, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Umberto Ulmanella
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Patent number: 8147770Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.Type: GrantFiled: November 24, 2010Date of Patent: April 3, 2012Assignee: Applied Biosystems, LLCInventors: Debjyoti Banerjee, Konrad Faulstich, Aldrich Lau, Umberto Ulmanella, Jun Xie
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Patent number: 8092664Abstract: The present teachings relate to microfluidic valves and pumping systems, which may be suitable for controlling and facilitating liquid flow. Electrodes are disposed proximately to volumes containing a liquid. The liquid flow can be facilitated by electrowetting forces. Processes for controlling the flow of liquids, as well as for pumping liquids, are also disclosed.Type: GrantFiled: June 3, 2010Date of Patent: January 10, 2012Assignee: Applied Biosystems LLCInventor: Umberto Ulmanella
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Patent number: 8062611Abstract: A fluid processing device is provided that includes a substrate, a plurality of fluid retainment regions formed in or on the substrate, and a barrier at least partially separating two or more of the fluid retainment regions. The barrier includes a mixture of a sequestering material and a reaction component. The reaction component can be at least one of a reactant, a reagent, a catalyst, an initiator, a promoter, a cofactor, an enzyme, a salt, or a combination thereof. The sequestering material can be a porous material, a dissolvable material, or both.Type: GrantFiled: October 18, 2005Date of Patent: November 22, 2011Assignee: Applied Biosystems, LLCInventors: Konrad Faulstich, Aldrich N. K. Lau, Debjyoti Banerjee, Umberto Ulmanella, Jun Xie
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Publication number: 20110236984Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures.Type: ApplicationFiled: January 4, 2011Publication date: September 29, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Hongye Sun, Eric S. Nordman, Mark F. Oldham, John R. O'Neill, Charles Connell, Umberto Ulmanella, Aldrich N.K. Lau, Theofilos Kotseroglou, Kenneth J. Livak
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Publication number: 20110126913Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.Type: ApplicationFiled: November 24, 2010Publication date: June 2, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Debjyoti Banerjee, Konrad Faulstich, Aldrich N. K. Lau, Umberto Ulmanella, Jun Xie
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Publication number: 20110114206Abstract: A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.Type: ApplicationFiled: November 12, 2010Publication date: May 19, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Debjyoti Banerjee, Umberto Ulmanella, Konrad Faulstich, Aldrich N.K. Lau, Jun Xie
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Publication number: 20110083964Abstract: The present teachings relate to microfluidic valves and pumping systems, which may be suitable for controlling and facilitating liquid flow. Electrodes are disposed proximately to volumes containing a liquid. The liquid flow can be facilitated by electrowetting forces. Processes for controlling the flow of liquids, as well as for pumping liquids, are also disclosed.Type: ApplicationFiled: June 3, 2010Publication date: April 14, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Umberto ULMANELLA
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Publication number: 20110020179Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.Type: ApplicationFiled: May 27, 2010Publication date: January 27, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Min Yue, David M. Liu, Yuh-Min Chiang, Joon-Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Joy Roy, Maryam Shariati, Umberto Ulmanella
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Publication number: 20100136701Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.Type: ApplicationFiled: November 30, 2009Publication date: June 3, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Debjyoti Banerjee, Konrad Faulstich, Aldrich N. K. Lau, Umberto Ulmanella, Jun Xie
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Publication number: 20100133104Abstract: A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.Type: ApplicationFiled: November 30, 2009Publication date: June 3, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Debjyoti Banerjee, Umberto Ulmanella, Konrad Faulstich, Aldrich N. K. Lau, Jun Xie