Abstract: A centrifuge rotor is described which facilitates the preparation of cell dispersions on microscope slides. The rotor is bowl-like in configuration and defines an annular channel having plural circumferentially disposed septa. The septa provide plural separate regions each adapted to receive removable chambers. The outer wall in each region is flat to accommodate a microscope slide. Each chamber has an outlet orifice adapted to contact the microscope slide. A sample containing blood cells, for example, may be placed in each chamber and the cells centrifugally sedimented against the slide associated with the respective chambers. A gasket at the interface between each slide and its chamber prevents leakage of the sample and a conduit in each chamber permits removal of the supernatant fluid following cell sedimentation.

Abstract: A blood bag is described which aids in maintaining the separation of blood components. This bag is constructed of a flexible plastic, has a conical top leading to an outlet line and internal septa to reduce swirling of the bag's contents during rotor deceleration. The bag is adapted to be placed in a split sleeve prior to placement in the swinging bucket of a centrifuge rotor. A collar is placed over the top of the bag to aid in reducing wrinkles in the bag.Methods are described for reducing contamination of separated blood components by reducing bag wrinkles, reducing swirling of the blood fractions during rotor deceleration, and reducing mixing during expression.

Abstract: A blood bag is described which aids in maintaining the separation of blood components. This bag is constructed of a flexible plastic, has a conical top leading to an outlet line and internal septa to reduce swirling of the bag's contents during rotor deceleration. The bag is adapted to be placed in a split sleeve prior to placement in the swinging bucket of a centrifuge rotor. A collar is placed over the top of the bag to aid in reducing wrinkles in the bag.Methods are described for reducing contamination of separated blood components by reducing bag wrinkles, reducing swirling of the blood fractions during rotor deceleration, and reducing mixing during expression.

Abstract: A blood bag is described which aids in maintaining the separation of blood components. This bag is constructed of a flexible plastic, has a conical top leading to an outlet line and internal septa to reduce swirling of the bag's contents during rotor deceleration. The bag is adapted to be placed in a split sleeve prior to placement in the swinging bucket of a centrifuge rotor. A collar is placed over the top of the bag to aid in reducing wrinkles in the bag.Methods are described for reducing contamination of separated blood components by reducing bag wrinkles, reducing swirling of the blood fractions during rotor deceleration, and reducing mixing during expression.

Abstract: A centrifuge rotor is described which facilitates the preparation of cell dispersions on microscope slides. The rotor is bowl-like in configuration and defines plural peripheral regions adapted to receive the slides and a removable sample chamber associated with each slide. Each chamber has an outlet adpated to contact its slide. A sample, containing blood cells, for example, may be placed in a chamber and the cells centrifugally sedimented against the slide associated therewith. Excess or unwanted fluid is removed from the chamber by applying a vacuum through a vacuum line and hollow drive shaft. Leaf springs maintain each chamber in position and the vacuum line in fluid contact with the chamber. The vacuum line conecting each chamber cooperates with its leaf spring such that if a chamber is missing, the leaf spring closes the vacuum line.

Abstract: Stoppers are separated from tubes they close by suspending a stoppered tube by its stopper, placing the suspended tube in a swinging bucket centrifuge, subjecting the suspended tube to centrifugal force.This may be accomplished by adapting a test tube rack to be placed in a centrifuge's swinging bucket type carrier. The rack is a two piece unit having upper and lower members that are separable. The test tubes use stoppers having an enlarged portion with a diameter which exceeds that of the test tube such that the tubes may be suspended from the upper member by their stoppers. The rack has a height which exceeds that of the test tubes such that when the rack with the tubes suspended therein are subjected to centrifugal force, the tubes withdraw from the stoppers. This leaves the stoppers positioned on the upper surface of the upper member. If now the upper member is lifted away, the stoppers are removed leaving the unstoppered test tubes positioned in the lower member of the rack.