Patents by Inventor Veronique Lecault
Veronique Lecault has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20220365072Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: ApplicationFiled: July 7, 2021Publication date: November 17, 2022Applicant: The University of British ColumbiaInventors: Marketa RICICOVA, Kevin Albert HEYRIES, Hans ZAHN, Oleh PETRIV, Veronique LECAULT, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad NELSON, Julie NIELSEN, Kathleen Lisaingo
-
Publication number: 20210269792Abstract: The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. The methods can also be used to identify an antigen that mediates a disease state, e.g., an autoantigen implicated in an autoimmune disorder or a tumor response.Type: ApplicationFiled: May 17, 2021Publication date: September 2, 2021Inventors: Ester FALCONER, Carl Lars Genghis HANSEN, Veronique LECAULT, Kathleen LISAINGO, Kevin Albert HEYRIES
-
Patent number: 11072772Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: GrantFiled: June 10, 2020Date of Patent: July 27, 2021Assignee: The University of British ColumbiaInventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Publication number: 20200363401Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: ApplicationFiled: July 16, 2020Publication date: November 19, 2020Applicant: The University of British ColumbiaInventors: Marketa RICICOVA, Kevin Albert HEYRIES, Hans ZAHN, Oleh PETRIV, Veronique LECAULT, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad NELSON, Julie NIELSEN, Kathleen Lisaingo
-
Publication number: 20200325431Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: ApplicationFiled: June 10, 2020Publication date: October 15, 2020Applicant: The University of British ColumbiaInventors: Carl L. G. Hansen, Veronique LECAULT, James M. PIRET, Anupam Singhal
-
Patent number: 10738270Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: GrantFiled: March 13, 2020Date of Patent: August 11, 2020Assignee: The University of British ColumbiaInventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Patent number: 10725024Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: GrantFiled: March 28, 2014Date of Patent: July 28, 2020Assignee: THE UNIVERSITY OF BRITISH COLUMBIAInventors: Marketa Ricicova, Kevin Albert Heyries, Hans Zahn, Oleh Petriv, Veronique Lecault, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad Nelson, Julie Nielsen, Kathleen Lisaingo
-
Publication number: 20200216791Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: ApplicationFiled: March 13, 2020Publication date: July 9, 2020Applicant: The University of British ColumbiaInventors: Carl L. G. Hansen, Veronique LECAULT, James M. PIRET, Anupam Singhal
-
Patent number: 10704018Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: GrantFiled: July 30, 2019Date of Patent: July 7, 2020Assignee: THE UNIVERSITY OF BRITISH COLUMBIAInventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Publication number: 20200156071Abstract: Methods and devices for identifying a cell population comprising an effector cell exhibiting an extracellular effect are provided. The method comprises retaining in a plurality of open chambers a plurality of cell populations, each optionally comprising one or more effector cells. The open chambers can each comprise a readout particle population, and the open chambers are present in a first component of a device comprising a first component and optionally a second component. The open chambers have an average aspect ratio of ?0.6 and the first component forms a reversible seal with the second component.Type: ApplicationFiled: March 17, 2017Publication date: May 21, 2020Inventors: Carl Lars Genghis Hansen, Kathleen Lisaingo, Veronique Lecault
-
Publication number: 20200149031Abstract: The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. The methods can also be used to identify an antigen that mediates a disease state, e.g., an autoantigen implicated in an autoimmune disorder or a tumor response.Type: ApplicationFiled: December 21, 2016Publication date: May 14, 2020Inventors: Ester FALCONER, Carl Lars Genghis HANSEN, Veronique LECAULT, Kathleen LISAINGO, Kevin Albert HEYRIES
-
Patent number: 10508299Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: GrantFiled: October 25, 2017Date of Patent: December 17, 2019Assignee: The University of British ColumbiaInventors: Carl Lars Genghis Hansen, Michael Vanlnsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
-
Publication number: 20190367860Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: ApplicationFiled: July 30, 2019Publication date: December 5, 2019Inventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Patent number: 10421936Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: GrantFiled: December 13, 2017Date of Patent: September 24, 2019Assignee: The University of British ColumbiaInventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Patent number: 10087408Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet.Type: GrantFiled: July 7, 2011Date of Patent: October 2, 2018Assignee: The University of British ColumbiaInventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Publication number: 20180230512Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: ApplicationFiled: October 25, 2017Publication date: August 16, 2018Inventors: Carl Lars Genghis Hansen, Michael VanInsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
-
Publication number: 20180163166Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.Type: ApplicationFiled: December 13, 2017Publication date: June 14, 2018Inventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
-
Patent number: 9902990Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: GrantFiled: May 27, 2011Date of Patent: February 27, 2018Assignee: The University of British ColumbiaInventors: Carl Lars Genghis Hansen, Michael VanInsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
-
Publication number: 20160252495Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: ApplicationFiled: March 28, 2014Publication date: September 1, 2016Inventors: Marketa Ricicova, Kevin Albert Heyries, Hans Zahn, Oleh Petriv, Veronique Lecault, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad Nelson, Julie Nielsen, Kathleen Lisaingo
-
Publication number: 20150018226Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: ApplicationFiled: May 27, 2011Publication date: January 15, 2015Inventors: Carl Lars Genoble Hansen, Michael Vaninsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski