Abstract: Analyzing expression of protein-coding variants in cells is provided herein. A method may include replacing a protein coding-region of the DNA in a cell with a donor vector including a variant of the protein-coding region and a first barcode identifying that variant. The cell may generate mRNA including an expression of the variant and an expression of the first barcode. A second barcode corresponding to the cell may be coupled to the mRNA. The mRNA. having the second barcode coupled thereto, may be reverse transcribed into complementary cDNA. The cDNA may be sequenced. The donor vector or cDNA may be sequenced using amplicon sequencing. The donor vector sequence and the cDNA sequence may be correlated to identify the variant and the cell's expression of the variant.

Abstract: A wireless implant and associated system for motor function recovery after spinal cord injury, and more particularly a multi-channel wireless implant with small package size. The wireless implant can further be used in various medical applications, such as retinal prostheses, gastrointestinal implant, vagus nerve stimulation, and cortical neuromodulation. The system also includes a method and its implementation to acquire the impedance model of the electrode-tissue interface of the implant.

Abstract: An ultrasonic transducer device includes a bottom electrode layer of a transducer cavity disposed over a substrate. The bottom electrode layer includes a bottom layer of a first type metal; a top layer of the first type metal; a second type metal disposed between the bottom layer and the top layer; and at least one intermediate layer of the first type metal disposed between the bottom layer and the top layer, the at least one intermediate layer configured so as to define at least two discrete layers of the second type metal.

Abstract: Systems and methods for defending against volumetric attacks, implemented in a cloud-based system. Embodiments include steps of, monitoring flows and a rate of requests to a Data Center (DC); receiving a request from an address to the DC, the request being for a service in a cloud-based system; determining if the address has been successfully authenticated within a past predetermined time period; responsive to the address not having been successfully authenticated within the past time period, and one of (i) the rate of requests being above a threshold or (ii) the number of flows being above a threshold, placing the address in a penalty box for a predetermined amount of time; and blocking requests from the address in the penalty box for the predetermined amount of time.

Abstract: A wireless implant and associated system for motor function recovery after spinal cord injury, and more particularly a multi-channel wireless implant with small package size. The wireless implant can further be used in various medical applications, such as retinal prostheses, gastrointestinal implant, vagus nerve stimulation, and cortical neuromodulation. The system also includes a method and its implementation to acquire the impedance model of the electrode-tissue interface of the implant.

Abstract: A system may have a display that includes a plurality of light sources such as light-emitting diodes. The display may be an exterior display that is routinely operated in daytime conditions where ambient light levels are very high. To increase contrast in an exterior display, the display may include a sunlight blocking element. A static sunlight blocking element may include a louver film with asymmetric light blocking portions. The system may include an ambient light sensor that is configured to determine ambient light levels. Based on the detected ambient light level, control circuitry in the system may adjust one or more adjustable components in the display. The display may include an adjustable diffuser that has at least two states with different haze levels. The display may include an adjustable tint layer that has at least two states with different transmission levels.

Abstract: A method for electronic device virtualization and management includes transmitting a signal to a server from a client. The signal is initiated by a user of a user interface of the client. The user interface of the client presents at least two devices to the user, which the user may interact with. The signal may include a power cycling instruction directed to at least one of the devices. When the server receives a communication back from the at least one device, it may send the information to the client, where the user interface may be updated in response to the communication. The devices may be controlled with other instructions, such as scheduling instructions, firmware update instructions, and configuration backup instructions. If a power device is virtualized, it may be controlled on a port-by-port basis.

Abstract: A rain erosion test device includes an integrated double-wing blade and a drive assembly. The integrated double-wing blade is symmetrical around the center of the integrated double-wing blade, and rotatable around the center in the horizontal plane. A rain erosion sample mold fixing mechanism is provided on a leading edge adjacent to each of two ends of the integrated double-wing blade. The drive assembly is configured to drive the integrated double-wing blade to rotate. The blade of the rain erosion test device has simple machining and a low cost, and can still operate stably when a weight difference between two ends of the blade is up to 1 kg so as to achieve a high error-tolerant rate. The rain erosion test device can realize continuous test on rain erosion resistance of the coating at a speed of 207 m/s or more.

Abstract: A process for joining polymer molded parts. Providing a first part made from a first material and a second part made from a same or different first material. Attaching an infrared or other thermal bonding material to at least one of the parts in a place for the parts to be joined. Thereafter, heating the at least on infrared bonding material with an infrared heating element and pressing the first part into contact with the second part wherein a bond is formed.

Abstract: Provided are methods for isolating T-cells with T cell receptors (TCRs) optimized for reactivity to specific peptides and decreased cross-reactivity to non-target peptides. Advantageously, TCRs of the invention can be optimized to target cancer antigens and peptides while having reducing reactivity to healthy cells. Methods of the invention utilize a novel combination of culturing conditions that increase T-cell activation and allow for validation of TCR activity. Culturing conditions of the invention further reduce culturing times generally needed to achieve expanded reactive T-cells. Because of the robust nature of the activation and validation conditions of the present invention, variants of identified TCRs can also be optimized and validated for their response to peptides, including cancer peptides.

Abstract: Provided are methods for isolating T-cells with T cell receptors (TCRs) optimized for reactivity to specific peptides and decreased cross-reactivity to non-target peptides. Advantageously, TCRs of the invention can be optimized to target cancer antigens and peptides while having reducing reactivity to healthy cells. Methods of the invention utilize a novel combination of culturing conditions that increase T-cell activation and allow for validation of TCR activity. Culturing conditions of the invention further reduce culturing times generally needed to achieve expanded reactive T-cells. Because of the robust nature of the activation and validation conditions of the present invention, variants of identified TCRs can also be optimized and validated for their response to peptides, including cancer peptides.

Abstract: Described herein are single chain trimer (SCT) polypeptides comprising or consisting essentially of a target peptide, a first linker, at least a portion of a beta-2 microglobulin domain, a second linker, and at least a portion of a major histocompatibility complex (MHC) I alpha chain, or pharmaceutically acceptable derivatives thereof. The SCT polypeptides may further include a leader peptide, e.g., a PHO5, SUC2, app8, or HLA A2 leader sequence at the N-terminus of the target peptide. Further described herein are polypeptide compositions comprising or consisting essentially of a first polypeptide comprising a target peptide, and a second polypeptide comprising at least a portion of a beta-2 microglobulin domain, a second linker, and at least a portion of a major histocompatibility complex (MHC) I alpha chain, a third linker, and a tether peptide, or pharmaceutically acceptable derivatives thereof. The first polypeptide and/or the second polypeptide may further include a leader peptide, e.g.

Abstract: Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) cryoprotectant: the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol, and ethylene glycol; (3) a saline buffer; wherein the salt buffer is a solution containing Na+, K+, Mg+, Cl?, and CH3COO? ions; (4) a vitamin; and (5) an amino acid, wherein the human albumin concentration is 1%-20% (w/v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used or conventional adherent and suspension cells.

Abstract: A process for joining polymer molded parts. Providing a first part made from a first material and a second part made from a same or different first material. Attaching an infrared or other thermal bonding material to at least one of the parts in a place for the parts to be joined. Thereafter, heating the at least on infrared bonding material with an infrared heating element and pressing the first part into contact with the second part wherein a bond is formed.

Abstract: In one embodiment, an apparatus includes a connecting member configured for positioning on an upper surface of an integrated circuit package and a cable comprising a first end attached to the connecting member and a second end configured for electrically coupling with a power supply component. The connecting member is operable to position the cable for connection to the upper surface of the integrated circuit package to deliver power from the power supply component to the integrated circuit package with the power supply component and the integrated circuit package mounted on an upper surface of a printed circuit board. A method is also disclosed herein.

Abstract: Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) cryoprotectant: the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol, and ethylene glycol; (3) a saline buffer; wherein the salt buffer is a solution containing Na+, K+, Mg+, Cl?, and CH3COO? ions; (4) a vitamin; and (5) an amino acid, wherein the human albumin concentration is 1%-20% (w/v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used or conventional adherent and suspension cells.

Abstract: Disclosed herein are system, method, and computer program product embodiments for detecting duplicates with exact and fuzzy matching on encrypted match indexes using an encryption key in a cloud computing platform. An embodiment operates by determining a match rule index value upon reception of a new record. The embodiment encrypts the match index rule value using the customer's encryption key and a deterministic encryption method and stores the encrypted match rule index value. Duplicate detection may be later performed by using the same deterministic encryption method to determine a cypher text for a candidate entry and comparing the ciphertext to the stored encrypted match indexes.

Abstract: Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) cryoprotectant: the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol, and ethylene glycol; (3) a saline buffer; wherein the salt buffer is a solution containing Na+, K+, Mg+, Cl?, and CH3COO? ions; (4) a vitamin; and (5) an amino acid, wherein the human albumin concentration is 1%-20% (w/v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used or conventional adherent and suspension cells.

Abstract: In a method for superimposed conveyance of data and electrical power, a first switch can cycle to convey the electric power and data from a first device. Binary digits of the data from the first device can be represented by transitions, of a voltage at a node of a second device, between a first voltage and a second voltage. A second switch can cycle to convey data from the second device. Binary digits of the data from the second device can be represented by transitions, of a current through a component of the first device, between being less than a threshold and being greater than the threshold. A voltage regulator of the second device can provide, in response to the voltage at the node being within a range of voltages that includes the first voltage and the second voltage, the electrical power to one or more components.

Abstract: A metamaterial-based phase shifting element utilizes a variable capacitor (varicap) to control the effective capacitance of a metamaterial structure in order to control the phase of a radio frequency output signal generated by the metamaterial structure. The metamaterial structure is configured to resonate at the same radio wave frequency as an incident input signal (radiation), whereby the metamaterial structure emits the output signal by way of controlled scattering the input signal. A variable capacitance applied on metamaterial structure by the varicap is adjustable by way of a control voltage, whereby the output phase is adjusted by way of adjusting the control voltage. The metamaterial structure is constructed using inexpensive metal film or PCB fabrication technology including an upper metal “island” structure, a lower metal backplane layer, and a dielectric layer sandwiched therebetween.