Abstract: The present disclosure envisages a method for producing 1,3-butadiene by a biochemical approach. The starting material used for the biosynthesis of 1,3-butadiene, i.e., malonyl-CoA, can be obtained by converting syngas to acetyl-CoA and further carboxylation to malonyl-CoA. The next step involves condensing malonyl-CoA and acetaldehyde via a decarboxylative Claisen condensation reaction, to obtain 3-hydroxybutyryl-CoA. Syngas, a byproduct of many industrial processes, is used here to produce 1,3-butadiene, which makes the method of the present disclosure economical, and produces a product having value addition.

Abstract: The present disclosure relates to method for enhanced production of metabolite including but not limiting to isoprene and isoprenoid through chromosomal integration of genes belonging to MEP pathway. The disclosure further relates a host cell for the production of the said metabolite. The method of the present disclosure bypasses the cumbersome method of plasmid application for the production of metabolite. The disclosure also relates to a gene construct comprising MEP genes and auxotrophic markers and a vector comprising the said gene construct.

Abstract: The present disclosure relates to a process for recovering a polyester component and products of a non-polyester component from a fabric containing a polyester blend. The polyester component of the present disclosure is polyethylene terephthalate and the non-polyester component is wool. The process includes hydrolyzing the article using at least one hydrolyzing agent to convert the non-polyester component into products of the non-polyester component and release the polyester component.

Abstract: Disclosed herein are methods and compositions related to the production and extraction of oils and biodiesel from oleaginous yeast, such as a new yeast isolate of the genus Pichia. Also disclosed herein are methods for providing fermentation conditions for the production of yeast in high density using inexpensive raw materials including crude glycerol and corn steep liquor.

Abstract: The present disclosure relates to the field of organic chemistry in general. Particularly, the present invention relates to a process for conversion of biomass to simpler organic compound(s) such as carbohydrates. The process of the present disclosure provides for pre-treatment of biomass using acids organic acids and/or inorganic acids and further treatment using de-esterification agent, thereby rendering the biomass more amenable to enzymatic hydrolysis. The present disclosure also relates to application of the process in biofuel production by fermenting the organic products obtained by the process of the disclosure.

Abstract: The present disclosure relates to a process for recovering a polyester component and products of a non-polyester component from an article containing a polyester blend. The polyester blend consists of a polyester component and a non-polyester component. Particularly, the polyester component of the present disclosure is polyethylene terephthalate and the non-polyester component is at least one selected from the group consisting of cotton and viscose. The process of the present disclosure includes hydrolyzing the article using at least one hydrolyzing agent having pH ranging from 7 to 14 to obtain a first mixture containing the polyester component, the non-polyester component, optionally, products of the non-polyester component and the hydrolyzing agent.

Abstract: The present disclosure relates to a process for recovering a polyester component and products of a non-polyester component from a fabric containing a polyester blend. The polyester component of the present disclosure is polyethylene terephthalate and the non-polyester component is wool. The process includes hydrolyzing the article using at least one hydrolyzing agent to convert the non-polyester component into products of the non-polyester component and release the polyester component.

Abstract: The production of butanol by the mutant Clostridium acetobutylicum MTCC 5587 using an acid pretreated biomass such as jatropha seed cake, pongamia seed cake and banana stems as renewable feedstock. Chemical mutagenesis was carried out for improvement of the strain for better butanol tolerance and production. This leads to a high yield of butanol obtained in single batch fermentation using acid pretreated jatropha seed cake.

Abstract: The present invention provides the use of acid pretreated biomass such as jatropha seed cake, pongamia seed cake and banana stems as renewable feedstock for butanol production by the mutant Clostridium acetobutylicum MTCC 587. Chemical mutagenesis was carried out for improvement of the strain for better butanol tolerance and production. This present invention for the first time has reported an efficient process for high yield of butanol obtained in single batch fermentation using acid pretreated jatropha seed cake.

Abstract: The disclosure provides methods and systems for the production of biodiesel from biological feedstocks such as vegetable oils or animal fats. In particular, the disclosure is directed to the product of biodiesel from seeds such as non-edible oil seeds. Methods for maximizing oil recovery from the feedstocks are provided, as well as methods to convert free fatty acids in the feedstocks into glycerides amenable to transesterification. Thus the methods and systems provide for efficient biodiesel production.

Abstract: Disclosed herein are methods and compositions related to the production and extraction of oils and biodiesel from Noleaginous yeast, such as a new yeast isolate of the genus Pichia. Also disclosed herein are methods for providing fermentation conditions for the production of yeast in high density using inexpensive raw materials including crude glycerol and corn steep liquor.

Abstract: The present invention provides an improved process for the production of gibberellic acid (GA3), achieving a yield over 225 g/kg of GA3 with solid substrate fermentation, and over 15 g/L by submerged fermentation. The method also provides novel substrates, including the use of Jatropha seed cake. The present invention has in particular provided an improved, cost-effective process for the manufacture of GA3, as the process has a surprisingly high yield of product, achieves the maximal yield in shorter time than other techniques, consumes less energy, and works with very inexpensive substrates. In all, the manufacturing costs are significantly reduced.

Abstract: The present invention relates to an efficient process for producing polylactic acid from fermentation of renewable agricultural feed-stocks not limited to molasses or cane bagasse employed as starting material. The present invention in particular provides a cost effective and industrially scalable process for producing polylactic acid obtained by fermentation of Lactic acid having industrial applications.

Abstract: The disclosure provides methods and systems for the production of biodiesel from biological feedstocks such as vegetable oils or animal fats. In particular, the disclosure is directed to the product of biodiesel from seeds such as non-edible oil seeds. Methods for maximizing oil recovery from the feedstocks are provided, as well as methods to convert free fatty acids in the feedstocks into glycerides amenable to transesterification. Thus the methods and systems provide for efficient biodiesel production.

Abstract: The present invention provides an efficient and improved process for the production and purification of high molecular weight hyaluronic acid (HA). One embodiment relates to providing growth conditions for production of high molecular weight HA in Streptococcus zooepidemicus. For example, growth in medium comprising a lower protein concentration, e.g., 1% casein hydrolyzate, and a higher sugar concentration, e.g., 5% sucrose, gave 5-6 g/L of high molecular weight HA in the range of 3.5-4.0×106 Da. The present invention also provides an efficient process for the purification of HA comprising treatment with silica gel then active carbon, and subsequent diafiltration with less volume of solvent.

Abstract: The present invention provides an improved process for the production of gibberellic acid (GA3), achieving a yield over 225 g/kg of GA3 with solid substrate fermentation, and over 15 g/L by submerged fermentation. The method also provides novel substrates, including the use of Jatropha seed cake. The present invention has in particular provided an improved, cost-effective process for the manufacture of GA3, as the process has a surprisingly high yield of product, achieves the maximal yield in shorter time than other techniques, consumes less energy, and works with very inexpensive substrates. In all, the manufacturing costs are significantly reduced.

Abstract: The present invention relates to an interactive wound cover wherein a cultured monolayer of keratinocytes is delivered using a biopolymer. The present invention describes the composition, method of preparation and its properties relating to safety, and efficacy. The wound cover of the present invention is useful in the treatment of wounds and in skin tissue regeneration.

Abstract: The present invention relates to an efficient process for producing polylactic acid from fermentation of renewable agricultural feed-stocks not limited to molasses or cane bagasse employed as starting material. The present invention in particular provides a cost effective and industrially scalable process for producing polylactic acid obtained by fermentation of Lactic acid having industrial applications.