Abstract: This disclosure provides a method to modify seed oil composition of Camelina sativa plants. The disclosure also provides novel promoters and gene sequences for modification of plant seed oil composition.

Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

Abstract: This disclosure provides a novel herbicide resistant plant, a method to transform Camelina sativa for herbicide resistance and a method for an improved in vitro regeneration of transformed plants.

Abstract: In order to better aid consumers of prescription medicine and the like in efficient and rapid identification of the correct pill to be taken from among multiple containers, a novel group of articles of manufacture adapted for use with end-use prescription medicine containers at least includes a plurality of predetermined medicine purpose types corresponding to different types of medical or vitality purposes that underlying medicines are targeted to treat or address. Each article of manufacture is adapted to be coupled to an end-use, target prescription medicine container by an end-user or pharmacy function, and each article of manufacture at least includes an ideogram adapted to encourage a psychological association with, and identification of, the medicine expected to be placed in the target prescription medicine container to which the article of manufacture is adapted, and each article of manufacture is associated with a particular medicine purpose type.

Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

Abstract: The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.

Abstract: The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

Abstract: An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed.

Abstract: The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

Abstract: The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.

Abstract: The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

Abstract: An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed.

Abstract: The invention is related to a method and a DNA construct for controlling transgene segregation in a sexually reproducing multicellular organism (SRMO), especially in such SRMOs which are susceptible of interbreeding with their cultured or wild-type relatives. The method and construct allows the farmer to reuse the transgenic crop without risk for escape of the transgene into the environment. The DNA construct is a recoverable block of function (RBF) system comprising at least one blocking construct (BC), and at least one means for recovering the blocked functions. The BC has a capacity of blocking at least one molecular or physiological function essential for the development and/or reproductive cycle of the SRMO. The BC is closely linked to the transgene of intereset (TGI). The blocked function is recoverable by an external treatment or intervention, which is optionally combined with one or more recovering constructs (RC).

Abstract: The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

Abstract: The invention is related to a method and a complex of DNA constructs for providing an increased level of control of transgene segregation in Sexually Reproducing Multicellular Organisms (SRMOs), which are prone to out-crossing with their wild-type or cultivated relatives. The method and constructs allow the farmer to reuse the transgenic crop. without risk. The RBF system comprises one or more Transgenes of Interest (TGIs) encoding desired gene products, one or more Blocking Constructs (BC), and one or more user-controlled means for recovering the blocked functions. The BC has the capacity of blocking at least one function essential for the survival and/or reproduction of the SRMO. Preferably more than one BC flanking the TGIs are used. The BC may be located in close proximity to the TGI, preferably in an intron or flanking the TGIs. The blocked function is recoverable by user-controlled interventions preferably applicable under confined conditions.

Abstract: The present invention relates to plant biotechnology and specifically to a novel transformation protocol for obtaining transgenic turnip rape plants with Agrobacterium mediated transformation. In the protocol an internode section of the inflorescence carrying stem of mature turnip rape is used as explant.

Abstract: The present invention is related to a method and a system for controlling the transgene segregation and spread. Escape of the gene of interest (TGI) into the environment is prevented by a repressible excision system (RES), which can be controlled by externally applicable means and comprises an excision construct (EC) having a gene encoding recombinase closely linked to the (TGI) and flanked by excision recognition sites (ERSs). The externally applicable means for controlling the repression of the expression of the gene encoding the recombinase enzyme is achieved with or without a repressor construct (RC). The action of the repressible excision system (RES) leads to excision of the transgenic insert, whenever a transgenic organism and the externally applicable means are withdrawn, which occurs if a transgenic organism escapes from the human control.