Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and characterization of novel NEDD8 activating and conjugating enzymes.

Abstract: Compositions are provided according to embodiments of the present invention which include an isolated fusion protein including an E3 protein RING motif bonded to an UCE E2 protein. In preferred embodiments, the C-terminus of the E3 protein RING motif is bonded to the N-terminus of the UCE E2 protein by an intervening peptide linker. Optionally, the peptide linker is 5-50 amino acids. Preferred is a peptide linker of 15-20 amino acids. Methods of identifying ubiquitylation modulators are provided according to embodiments of the present invention which include contacting an isolated fusion protein including an E3 protein RING motif bonded to an UCE E2 protein with a ubiquitylation substrate in the presence of a test substance. Ubiquitylation of the ubiquitylation substrate is then detected to determine the effect of the test substance on ubiquitylation of the ubiquitylation substrate.

Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and characterization of novel NEDD8 activating and conjugating enzymes.

Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and charactization of novel NEDD8 activating and conjugating enzymes.

Abstract: Compositions are provided according to embodiments of the present invention which include an isolated fusion protein including an E3 protein RING motif bonded to an UCE E2 protein. In preferred embodiments, the C-terminus of the E3 protein RING motif is bonded to the N-terminus of the UCE E2 protein by an intervening peptide linker. Optionally, the peptide linker is 5-50 amino acids. Preferred is a peptide linker of 15-20 amino acids. Methods of identifying ubiquitylation modulators are provided according to embodiments of the present invention which include contacting an isolated fusion protein including an E3 protein RING motif bonded to an UCE E2 protein with a ubiquitylation substrate in the presence of a test substance. Ubiquitylation of the ubiquitylation substrate is then detected to determine the effect of the test substance on ubiquitylation of the ubiquitylation substrate.

Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and characterization of novel NEDD8 activating and conjugating enzymes.

Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and charactization of novel NEDD8 activating and conjugating enzymes.

Abstract: The present invention relates to an apparatus for remotely controlling a vehicle cover that extends and retracts the cover automatically. The apparatus for remotely controlling a vehicle cover comprises, a plurality of covers for covering a vehicle, a plurality of wires for guiding the cover to extend and retract from and to a housing, a plurality of reel tables placed on the vehicle to route and secure the wires and the cover; and a control module comprising. The control module comprises at least one motor for extending and retracting the covers by controlling the wires; a means for receiving wireless control signals from a user operated remote control means; and a means for interpreting and implementing the signals.

Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and characterization of novel NEDD8 activating and conjugating enzymes.

Abstract: The invention relates to covalent modification of proteins through their conjugation with other proteins. More particularly, the invention relates to the modulation of such conjugation involving the protein NEDD8. The invention provides compositions and methods for detecting and/or modulating the activation and/or conjugation of NEDD8, as well as compositions and methods for discovering molecules which are useful in detecting and/or modulating the activation and/or conjugation of NEDD8. The present invention arises from the purification and characterization of novel NEDD8 activating and conjugating enzymes.