Patents by Inventor Virginia Espina
Virginia Espina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240158465Abstract: In an embodiment, present invention relates to a method of generating, ex vivo production of soluble Lox-1 (sLox-1), comprising: introducing a sample containing blood into a device; adding a coagulation enhancing material in the sample to form a cultured blood clot; incubating the cultured blood clot in the device at a temperature greater than 25° C. and less than 45° C. for at least 2 hours to allow production of Lox-1 from neutrophils of blood and to shed the sLox-1 outside the cultured blood clot; and collecting sLox-1 shedded in the device, wherein the method is configured to shed sLox-1 more than fresh blood.Type: ApplicationFiled: November 22, 2023Publication date: May 16, 2024Applicant: GEORGE MASON UNIVERSITYInventors: Caroline Dieckmann Hoemann, Julia Alexa Leonard, Virginia A. Espina, David Kepplinger
-
Publication number: 20240110852Abstract: Compositions and methods for the simulataneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: ApplicationFiled: September 1, 2023Publication date: April 4, 2024Applicant: GEORGE MASON RESEARCH FOUNDATION, INC.Inventors: Lance LIOTTA, Virginia ESPINA, Nitin AGRAWAL, Alessandra Luchini KUNKEL
-
Publication number: 20230416799Abstract: An embodiment of the invention provides a method of generating, ex vivo production of soluble Lox-1 (sLox-1), comprising: introducing a sample containing a blood free from an anti-coagulant factor into a device; adding a coagulation enhancing material in the sample; incubating the device; forming a cultured blood clot in the device; and shedding of the sLox-1 outside the cultured blood clot, wherein the method is configured to shed sLox-1 more than an anti-coagulated blood.Type: ApplicationFiled: May 22, 2023Publication date: December 28, 2023Applicant: GEORGE MASON UNIVERSITYInventors: Caroline Dieckmann Hoemann, Julia Alexa Leonard, Virginia A. Espina, David Kepplinger
-
Publication number: 20230384187Abstract: A liquid including an organic component, a stabilization component, and a preservation component is provided. The organic component, the stabilization component, and the preservation component are different components. The organic component present in the liquid includes a volatile organic solution with a refractive index of 1.5 or below. Further, the liquid is a visualization solution that provides a resolving power of a lens by 1/1.5.Type: ApplicationFiled: November 10, 2021Publication date: November 30, 2023Applicant: George Mason Research Foundation, Inc.Inventors: Lance A. LIOTTA, Alessandra Luchini KUNKEL, Marissa Ashton HOWARD, Amanda Haymond STILL, Amanda Lynn STILL, Virginia ESPINA
-
Patent number: 11747242Abstract: Compositions and methods for the simultaneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: GrantFiled: May 20, 2020Date of Patent: September 5, 2023Assignee: George Mason Research Foundation, Inc.Inventors: Lance Liotta, Virginia Espina, Nitin Agrawal, Alessandra Luchini Kunkel
-
Patent number: 11493518Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.Type: GrantFiled: September 25, 2018Date of Patent: November 8, 2022Assignee: GEORGE MASON RESEARCH FOUNDATION, INC.Inventors: Alessandra Luchini Kunkel, Lance Liotta, Virginia Espina
-
Patent number: 10927155Abstract: In one aspect, the invention relates to compositions comprising peptides and/or peptidomimetic compounds, methods of making same, pharmaceutical compositions comprising same, and methods of treating various diseases, including, but not limited to, arthritis, obstructive lung disease, psoriasis, asthma, a defect in hematopoiesis, a neoplasia, a fungal infection, a parasitic infection, or an autoimmune disease, such as, but not limited to, rheumatoid arthritis, atopic allergy, anaphylaxis, psoriasis, asthma, lupus erythematosis, a myeloid cell disorder, or a eosinophil cell disorder. In an aspect, the disclosed peptides and/or compounds inhibit the interaction of IL-1RAcP with the ST2 and IL33 receptor/cytokine complexes. In a further aspect, the disclosed peptides and/or compounds inhibit the interaction of IL-1RAcP with the IL-1R1 and IL-1? receptor/cytokine complexes.Type: GrantFiled: November 18, 2016Date of Patent: February 23, 2021Assignee: GEORGE MASON RESEARCH FOUNDATION, INC.Inventors: Lance Liotta, Alessandra Luchini, Virginia Espina, Mikell Paige
-
Publication number: 20200333220Abstract: Compositions and methods for the simulataneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: ApplicationFiled: May 20, 2020Publication date: October 22, 2020Applicant: GEORGE MASON RESEARCH FOUNDATION, INC.Inventors: Lance LIOTTA, Virginia ESPINA, Nitin AGRAWAL, Alessandra Luchini KUNKEL
-
Patent number: 10697866Abstract: Compositions and methods for the simulataneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: GrantFiled: April 26, 2019Date of Patent: June 30, 2020Assignee: GEORGE MASON RESEARCH FOUNDATION, INC.Inventors: Lance Liotta, Virginia Espina, Nitin Agrawal, Alessandra Luchini
-
Publication number: 20190352351Abstract: In one aspect, the invention relates to compositions comprising peptides and/or peptidomimetic compounds, methods of making same, pharmaceutical compositions comprising same, and methods of treating various diseases, including, but not limited to, arthritis, obstructive lung disease, psoriasis, asthma, a defect in hematopoiesis, a neoplasia, a fungal infection, a parasitic infection, or an autoimmune disease, such as, but not limited to, rheumatoid arthritis, atopic allergy, anaphylaxis, psoriasis, asthma, lupus erythematosis, a myeloid cell disorder, or a eosinophil cell disorder. In an aspect, the disclosed peptides and/or compounds inhibit the interaction of IL-1RAcP with the ST2 and IL33 receptor/cytokine complexes. In a further aspect, the disclosed peptides and/or compounds inhibit the interaction of IL-1RAcP with the IL-1R1 and IL-1? receptor/cytokine complexes.Type: ApplicationFiled: November 18, 2016Publication date: November 21, 2019Inventors: Lance LIOTTA, Alessandra LUCHINI, Virginia ESPINA, Mikell PAIGE
-
Publication number: 20190250079Abstract: Compositions and methods for the simulataneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: ApplicationFiled: April 26, 2019Publication date: August 15, 2019Inventors: LANCE LIOTTA, VIRGINIA ESPINA, NITIN AGRAWAL, ALESSANDRA LUCHINI
-
Patent number: 10324008Abstract: Compositions and methods for the simultaneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: GrantFiled: December 22, 2016Date of Patent: June 18, 2019Assignee: GEORGE MASON RESEARCH FOUNDATIONInventors: Lance Liotta, Virginia Espina, Nitin Agrawal, Alessandra Luchini
-
Publication number: 20190033319Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.Type: ApplicationFiled: September 25, 2018Publication date: January 31, 2019Applicant: George Mason Research Foundation, Inc.Inventors: Alessandra Luchini, Lance Liotta, Virginia Espina
-
Patent number: 10126304Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.Type: GrantFiled: January 15, 2014Date of Patent: November 13, 2018Assignee: George Mason Research Foundation, Inc.Inventors: Alessandra Luchini, Lance Liotta, Virginia Espina
-
Publication number: 20170176301Abstract: Compositions and methods for the simultaneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.Type: ApplicationFiled: December 22, 2016Publication date: June 22, 2017Inventors: Lance Liotta, Virginia Espina, Nitin Agrawal, Alessandra Luchini
-
Publication number: 20150338417Abstract: The present disclosure relates to compositions and methodology for revealing binding sites between proteins, proteins and nucleic acids, or proteins and small molecules. The disclosure provides rapid and direct positive identification and sequencing of the contact region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.Type: ApplicationFiled: January 15, 2014Publication date: November 26, 2015Inventors: Alessandra Luchini, Lance Liotta, Virginia Espina
-
Patent number: 9096833Abstract: Described herein are progenitor cancer cells and cell lines isolated from human breast ductal carcinoma in situ (DCIS) lesions and the uses of these cells or cell lines in drug design, drug screening, and monitoring in vivo therapy. The DCIS malignant precursor cells or cell lines are epithelial in origin, are positive for markers of autophagy, show at least one genetic difference from normal cells of said fragment, form 3-D tube-like structures or ball aggregates, or are inhibited in formation of 3-D structures and migration by treatment with chloroquine. In one embodiment, there is a loss of heterozygosity (LOH) that is narrowly confined to a region of chromosome 6p (6p21.1-6p12.3) that contains the SUPT3H gene.Type: GrantFiled: October 28, 2010Date of Patent: August 4, 2015Assignee: GEORGE MASON UNIVERSITYInventors: Virginia Espina, Lance Liotta
-
Patent number: 9086414Abstract: The present invention provides reliable methods to identify subsets of subjects with a cancer of epithelial origin characterized by a high level of phosphorylated c-erbB2 which does not correlate with the over-expression of total c-erbB2 as measured by IHC or FISH, for selection and inclusion for c-erbB2-direct treatment and therapy. Furthermore, the present invention provides a reliable method to determine whether a subject with a cancer of epithelial origin who has been determined to be c-erbB2 positive by IHC and by FISH should be excluded from c-erbB2-direct treatment because of a non-significant level of phosphorylated c-erbB2 in epithelial tumor tissue.Type: GrantFiled: July 8, 2009Date of Patent: July 21, 2015Assignee: George Mason Research Foundation, Inc.Inventors: Emanuel F. Petricoin, III, Lance A. Liotta, Virginia Espina, Julia D. Wulfkuhle
-
Patent number: 9029076Abstract: This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that stabilizes the proteins in the sample and that has a sufficient water content for a stabilizer and/or a permeability enhancing agent to be soluble therein; (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; (3) a permeability enhancing agent; and (4) lactic acid. Methods and kits are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g.Type: GrantFiled: March 15, 2013Date of Patent: May 12, 2015Assignee: George Mason Research Foundation, Inc.Inventors: Virginia A. Espina, Lance A. Liotta, David Geho
-
Patent number: 9012240Abstract: An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle.Type: GrantFiled: August 26, 2009Date of Patent: April 21, 2015Assignee: George Mason Research Foundation, Inc.Inventors: Lance A. Liotta, Alessandra Luchini, Emanuel F. Petricoin, Virginia Espina