Patents by Inventor Vladimir I. Slepnev
Vladimir I. Slepnev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240140981Abstract: The present disclosure, in some aspects, is directed to nucleic acid purification methods using a water-immiscible solvent wash. In other aspects, the present disclosure is directed to kits, components, and compositions (such as consumables) useful for the nucleic acid purification methods described herein.Type: ApplicationFiled: December 28, 2021Publication date: May 2, 2024Inventors: Vladimir I. SLEPNEV, Katie BOND, James MISSELBROOK, Anna Katherine RODGERS
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Publication number: 20220339623Abstract: The present disclosure, in some aspects, provides devices useful for determining a target nucleic acid profile of a sample. The device comprises an enrichment module, a reaction module and a nucleic acid sequencer. In other aspects, the present disclosure provides kits, components and compositions (such as consumables) of the devices and methods of using the devices described herein.Type: ApplicationFiled: September 29, 2020Publication date: October 27, 2022Inventors: Frederick Edward TURNER, Vladimir I. SLEPNEV, Ronald TEDESCO
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Publication number: 20220325322Abstract: The present disclosure, in some aspects, is directed to methods of nucleic acid enrichment using high affinity capture probes comprising one or more nucleotide analogs and uses thereof. For example, in another aspect, provided herein are methods of enriching a target nucleic acid if present in a sample. In another aspect, provided herein are methods of detecting if a target nucleic acid is in a sample. In another aspect, the present disclosure is directed to uses, kits, and compositions of the methods described herein.Type: ApplicationFiled: July 31, 2020Publication date: October 13, 2022Inventors: Vladimir I. SLEPNEV, Manasi RAJE, Nidhi GUPTA, Katie BOND, Frederick Edward TURNER
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Publication number: 20220298573Abstract: The present disclosure, in some aspects, is directed to methods of determining an infection profile of an individual, wherein the infection profile is based on a host response profile and a pathogen profile of the individual. In other aspects, the present disclosure is directed to uses (e.g., methods of treatment, treatment selection, patient selection), kits, and compositions of the methods described herein.Type: ApplicationFiled: June 10, 2020Publication date: September 22, 2022Inventors: Frederick Edward TURNER, Vladimir I. SLEPNEV
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Patent number: 9856514Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.Type: GrantFiled: November 18, 2014Date of Patent: January 2, 2018Assignee: Meridian Bioscience, Inc.Inventors: Vladimir I Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
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Publication number: 20170247680Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.Type: ApplicationFiled: April 4, 2017Publication date: August 31, 2017Inventors: Vladimir I. Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
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Publication number: 20150079601Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.Type: ApplicationFiled: November 18, 2014Publication date: March 19, 2015Inventors: Vladimir I. Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
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Publication number: 20140256029Abstract: An apparatus for expression profiling analysis, subjecting biological materials to polynucleotide extraction, amplification and analysis. The apparatus include an amplification device which permits the amplification of polynucleotides and an analysis device which quantifies the amount of the amplified polynucleotide products. The amplification device of the apparatus may further permit polynucleotide extraction to prepare the template for amplification, or sequence identification of a quantified polynucleotide product. A fraction collector may be included in the apparatus to collect a qualified polynucleotide product before its sequence is identified. The analysis device may further permit data generation, or alternatively, data can be generated by a separate data generation device provided with the apparatus. The devices within the apparatus are connected by connecting means which permit the transfer of a fluid or a signal for amplification and analysis.Type: ApplicationFiled: January 13, 2014Publication date: September 11, 2014Applicant: PRIMERADX, INC.Inventor: Vladimir I. Slepnev
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Publication number: 20130210013Abstract: The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.Type: ApplicationFiled: April 18, 2012Publication date: August 15, 2013Applicant: PRIMERADX, INC.Inventor: Vladimir I. Slepnev
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Publication number: 20130053274Abstract: Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards utilizing a plurality of nucleic acid templates that are amplified within the same reaction tube as the nucleic acid target(s) interrogated.Type: ApplicationFiled: October 19, 2012Publication date: February 28, 2013Applicant: PRIMERADX, INC.Inventors: Kyle Hart, Vladimir Dancik, Michael Bristol, Patrick Wells, Elizabeth Garcia, Vladimir I. Slepnev, John Unger
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Patent number: 8182995Abstract: The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.Type: GrantFiled: January 13, 2010Date of Patent: May 22, 2012Assignee: Primeradx, Inc.Inventor: Vladimir I. Slepnev
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Publication number: 20120100600Abstract: An apparatus for expression profiling analysis, subjecting biological materials to polynucleotide extraction, amplification and analysis. The apparatus include an amplification device which permits the amplification of polynucleotides and an analysis device which quantifies the amount of the amplified polynucleotide products. The amplification device of the apparatus may further permit polynucleotide extraction to prepare the template for amplification, or sequence identification of a quantified polynucleotide product. A fraction collector may be included in the apparatus to collect a qualified polynucleotide product before its sequence is identified. The analysis device may further permit data generation, or alternatively, data can be generated by a separate data generation device provided with the apparatus. The devices within the apparatus are connected by connecting means which permit the transfer of a fluid or a signal for amplification and analysis.Type: ApplicationFiled: December 6, 2011Publication date: April 26, 2012Applicant: PRIMERADX, INC.Inventor: Vladimir I. Slepnev
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Publication number: 20110300537Abstract: The invention relates to methods of genotyping single nucleotide differences in a nucleic acid sample. More particularly, the invention provides methods of identifying the nucleotide at a polymorphic site or a group of polymorphic sites in a sample of genomic DNA. The method uses tagged primer extension in which a set of tag sequences correspond to the identity of the nucleotides at the polymorphic sites. Primer extension products are PCR amplified using a common set of tag-specific primers, the downstream primers bearing distinguishable labels. Following separation by size and/or charge, the detection of distinguishable label in a product of the anticipated size determines the identity of the nucleotide at the polymorphic site. The method is well-suited for the genotyping of multiple single-nucleotide differences in one series of reactions.Type: ApplicationFiled: May 2, 2011Publication date: December 8, 2011Applicant: PRIMERADX, INC.Inventor: Vladimir I. Slepnev
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Publication number: 20100221725Abstract: The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.Type: ApplicationFiled: January 13, 2010Publication date: September 2, 2010Applicant: PRIMERADX, INC.Inventor: Vladimir I. Slepnev
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Patent number: 7674582Abstract: The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.Type: GrantFiled: June 13, 2006Date of Patent: March 9, 2010Assignee: Primeradx, Inc.Inventor: Vladimir I. Slepnev
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Patent number: 7550266Abstract: The invention provides compositions, methods and systems for dynamic transcription profiling of two or more samples. The method of the invention comprises the uses of sample-specific primers for cDNA synthesis and for subsequent amplification of the synthesized cDNAs. The levels of abundance of genes are compared between samples for the identification of differentially expressed genes.Type: GrantFiled: December 23, 2005Date of Patent: June 23, 2009Assignee: Primera Biosystems, Inc.Inventor: Vladimir I. Slepnev
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Publication number: 20090075274Abstract: The invention allows for the quantitative detection of a plurality of pathogens in a single sample. The method includes the amplification of a sample with a plurality of pathogen-specific primer pairs to generate amplicons of distinct sizes from each of the pathogen specific primer pairs. The method further includes the use of a plurality of competitor polynucleotide targets that correspond to each of the pathogen-specific primer pairs. The competitor polynucleotides are added to the reaction mixture at a known concentration to allow for the quantitation of the amount of pathogen in the sample. The method can be used for monitoring pathogen infection in an individual, preferably an immunocompromised individual.Type: ApplicationFiled: June 13, 2008Publication date: March 19, 2009Applicant: PRIMERA BIOSYSTEMS, INC.Inventors: Vladimir I. SLEPNEV, Kazumi SHIOSAKI, Kyle HART, Elizabeth GARCIA
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Patent number: 7445893Abstract: The invention provides sampling methods that permit the quantitative analysis of nucleic acid amplification reactions. The methods disclosed permit quantitative expression analysis of multiple genes or transcription units, both in the same amplification reaction and in multiple amplification reactions. In one aspect, the methods disclosed include, briefly, dispensing or withdrawing an aliquot from a reaction mixture at plural stages of an amplification regimen, separating and detecting nucleic acids in the aliquot, determining the quantity of a plurality of separated nucleic acid species in the aliquot, and for each separated nucleic acid species from each stage, correlating the quantity of the species with the stage at which the aliquot comprising the species was dispensed, thereby generating a profile of the amplification.Type: GrantFiled: November 21, 2003Date of Patent: November 4, 2008Assignee: Primera Biosystems, Inc.Inventor: Vladimir I. Slepnev
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Patent number: 7368246Abstract: Described herein are approaches to the measurement of gene expression for chosen genes in a biological sample. The methods permit the quantitation of target nucleic acids, e.g., DNAs or RNAs in a nucleic acid sample, both singly and in a multiplex format that permits the determination of levels (e.g., expression levels or copy numbers) for two or more target nucleic acids in a single reaction.Type: GrantFiled: September 15, 2005Date of Patent: May 6, 2008Assignee: Primera Biosystems, Inc.Inventors: Vladimir I. Slepnev, Elizabeth Garcia
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Patent number: 7081339Abstract: The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.Type: GrantFiled: March 12, 2003Date of Patent: July 25, 2006Assignee: Primera BioSystems, Inc.Inventor: Vladimir I. Slepnev