Patents by Inventor Volker Busskamp
Volker Busskamp has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117312Abstract: Forced expression of a handful of transcription factors (TFs) can induce conversion between cell identities; however, the extent to which TFs can alter cell identity has not been systematic ally assessed. Here, we assembled a “human TFome,” a comprehensive expression library of 1,578 human TF clones with fall coverage of the major TF families. By systematically screening the human TFome, we identified many individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression pantiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.Type: ApplicationFiled: May 18, 2023Publication date: April 11, 2024Applicant: President and Fellows of Harvard CollegeInventors: Hon Man Alex Ng, George M. Church, Volker Busskamp
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Patent number: 11845960Abstract: Forced expression of a handful of transcription factors (TFs) can induce conversions between cell identities; however, the extent to which TFs can alter cell identity has not been systematically assessed. Here, we assembled a human TFome, a comprehensive expression library of 1,578 human TF clones with full coverage of the major TF families. By systematically screening the human TFome, we identified 77 individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression profiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.Type: GrantFiled: September 12, 2017Date of Patent: December 19, 2023Assignee: President and Fellows of Harvard CollegeInventors: Hon Man Alex Ng, George M. Church, Volker Busskamp
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Publication number: 20220056402Abstract: A method for producing induced photoreceptor cells from an initial cell is provided, the method includes providing one or more transcription factors (TFs) including at least GON4L to the initial cell. In some versions, the initial cell is a human induced pluripotent stem cell (iPSC). In other embodiments the method includes providing the TFs OTX2 and/or NEUROD1 to the initial cell. Cells produced and obtainable by the method are also provided, the use of these cells as a medicament in the treatment of retinopathy, vectors for inducing the photoreceptor cells and combinations of transcription factors intended for this use.Type: ApplicationFiled: March 2, 2020Publication date: February 24, 2022Applicant: Rheinische Friedrich-Wilhelms-Universitat BonnInventors: Volker Busskamp, Marta Zuzic, Anka Kempe
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Patent number: 10987404Abstract: The present invention relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene.Type: GrantFiled: November 13, 2017Date of Patent: April 27, 2021Assignee: FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCHInventors: David Balya, Volker Busskamp, Pamela Lagali, Botond Roska
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Publication number: 20200063105Abstract: Forced expression of a handful of transcription factors (TFs) can induce conversions between cell identities; however, the extent to which TFs can alter cell identity has not been systematically assessed. Here, we assembled a “human TFome,” a comprehensive expression library of 1,578 human TF clones with full coverage of the major TF families. By systematically screening the human TFome, we identified many individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression profiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.Type: ApplicationFiled: April 30, 2018Publication date: February 27, 2020Applicant: President and Fellows of Harvard CollegeInventors: Hon Man Alex Ng, George M. Church, Volker Busskamp
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Publication number: 20190233795Abstract: Forced expression of a handful of transcription factors (TFs) can induce conversions between cell identities; however, the extent to which TFs can alter cell identity has not been systematically assessed. Here, we assembled a human TFome, a comprehensive expression library of 1,578 human TF clones with full coverage of the major TF families. By systematically screening the human TFome, we identified 77 individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression profiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.Type: ApplicationFiled: September 12, 2017Publication date: August 1, 2019Applicant: President and Fellows of Harvard CollegeInventors: Alex H. M. Ng, George M. Church, Volker Busskamp
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Publication number: 20180208928Abstract: The present inventions relates to isolated nucleic acid molecules comprising a nucleotide sequence coding for miRNA-182 (uuuggcaaugguagaacucacacu or ugguucuagacuugccaacua), miRNA-96 (uuuggcacuagcacauuuuugcu or aaucaugugcagugccaauaug) and/or miRNA-183 (uauggcacugguagaauucacu or gugaauuaccgaagggccauaa) for use in treating or ameliorating a ciliopathy and/or a photoreceptor dysfunction.Type: ApplicationFiled: December 6, 2017Publication date: July 26, 2018Applicant: FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCHInventors: Volker Busskamp, Witold Filipowicz, Jacek Krol, Botond Roska
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Publication number: 20180125925Abstract: The present invention relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene.Type: ApplicationFiled: November 13, 2017Publication date: May 10, 2018Applicant: FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCHInventors: David BALYA, Volker BUSSKAMP, Pamela LAGALI, Botond ROSKA
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Patent number: 9844579Abstract: The present inventions relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene.Type: GrantFiled: January 25, 2016Date of Patent: December 19, 2017Assignee: Friedrich Miescher Institute for Biomedical ResearchInventors: David Balya, Volker Busskamp, Pamela Lagali, Botond Roska
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Publication number: 20160304865Abstract: The present inventions relates to isolated nucleic acid molecules comprising a nucleotide sequence coding for mi RNA-182 (uuuggcaaugguagaacucacacu or ugguucuagacuugccaacua), miRNA-96 (uuuggcacuagcacauuuuugcu or aaucaugugcagugccaauaug) and/or mi RNA-183 (uauggcacugguagaauucacu or gugaauuaccgaagggccauaa) for use in treating or ameliorating a ciliopathy and/or a photoreceptor dysfunction.Type: ApplicationFiled: September 25, 2014Publication date: October 20, 2016Applicant: Friedrich Miescher Institute for Biomedical ResearchInventors: Volker Busskamp, Witold Filipowicz, Jacek Krol, Botond Roska
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Publication number: 20130059374Abstract: The present inventions relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene.Type: ApplicationFiled: September 13, 2012Publication date: March 7, 2013Inventors: Volker BUSSKAMP, Pamela LAGALI, Botond ROSKA, Rita HABAR
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Publication number: 20120258530Abstract: The present inventions relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene.Type: ApplicationFiled: November 18, 2011Publication date: October 11, 2012Inventors: David BALYA, Rita HABAR, Volker BUSSKAMP, Pamela LAGALI, Botond ROSKA
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Publication number: 20120196281Abstract: The present invention provides an organotypic system comprising a portion of a retina containing live cells in a cultured in medium, wherein photosensitivity has been restored in at least some of said live cells. Methods of use thereof are also provided.Type: ApplicationFiled: September 28, 2010Publication date: August 2, 2012Inventors: Volker Busskamp, Jens Duebel, Serge Picaud, Botond Roska, José Alain Sahel