Abstract: Improved holder specially adapted for disposable floor mats to be placed adjacent entryways to factories, industrial buildings, or the like or for use in connection with clean rooms, hospitals, laboratories. The holder includes a flat base adapted to hold the mat and at least one hingedly connected side flap adapted to fold over on top of an edge of the mat and securely retain it in position. Means are included to hold the flap against the mat in a closed position to firmly resist movement that would cause wrinkles or bunches in the mat which could cause trips or falls. In accordance with the invention, the base support includes at least one hand hole adjacent the edge of the base and located so as to be covered by the flap in the closed position. By means of the hand hole, direct pressure may be applied directly against the flap or by pressing against the mat so as to overcome the holding means and greatly facilitate opening of the flap to release the mat when desired.