Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

Abstract: A forceps for clamping, grasping, manipulating, coagulating/desiccating and cutting tissue includes a shaft portion having a proximal end, a distal end and first and second jaw members pivotally attached to the distal end of the shaft by a pivot assembly. Each of the jaw members including an opposing inner facing surface having a plurality of wave forms disposed thereon for engaging tissue therebetween. The wave forms disposed on the inner facing surface of the second jaw member being complimentary to the wave forms on the inner facing surface of first jaw member. A handle portion having an actuating assembly is attached to the proximal end of the shaft for imparting movement of the first and second jaw members from a first open position wherein the jaw members are disposed in spaced relation relative to one another to a second clamping position wherein the jaw members cooperate to grasp tissue therebetween.

Abstract: A laparoscopic bipolar electrosurgical instrument for sealing tissue includes a handle having an elongated tube affixed thereto. The tube includes first and second jaw members having electrically conductive sealing surfaces attached to a distal end thereof which are movable from a first position for approximating tissue to a second position for grasping tissue therebetween. The handle includes a fixed handle and a handle which is movable relative to the fixed handle to effect movement of the jaw members from the first position to the second position for grasping tissue. The jaw members connect to a source of electrosurgical energy such that the opposable sealing surfaces are capable of conducting electrosurgical energy through tissue held therebetween. A stop is included for maintaining a minimum separation distance between opposing sealing surfaces. A ratchet is also included to maintain a closure force in the range of about 7 kg/cm2 to about 13 kg/cm2 between opposing sealing surfaces.

Abstract: A bipolar electrosurgical instrument for clamping, grasping, manipulating, and sealing tissue includes first and second shafts each having a jaw member extending from a distal end thereof and a handle disposed at a proximal end thereof. The handle being operable to effect movement of the jaw members relative to one another from a first position wherein the jaw members are disposed in spaced relation relative to one another to a second position wherein the jaw members cooperate to grasp tissue therebetween. The bipolar instrument is connectable to a source of electrical energy having a first electrical potential connected to one of the jaw members and a second electrical potential connected to the other of the jaw members such that the jaw members are capable of selectively conducting energy through tissue held therebetween to effect a seal. Both the first and second electrical potentials are transmitted to the jaw members through the first shaft.

Abstract: A laparoscopic bipolar electrosurgical instrument for sealing tissue includes a handle having an elongated tube affixed thereto. The tube includes first and second jaw members having electrically conductive sealing surfaces attached to a distal end thereof which are movable from a first position for approximating tissue to a second position for grasping tissue therebetween. The handle includes a fixed handle and a handle which is movable relative to the fixed handle to effect movement of the jaw members from the first position to the second position for grasping tissue. The jaw members connect to a source of electrosurgical energy such that the opposable sealing surfaces are capable of conducting electrosurgical energy through tissue held therebetween. A stop is included for maintaining a minimum separation distance between opposing sealing surfaces. A ratchet is also included to maintain a closure force in the range of about 7 kg/cm2 to about 13 kg/cm2 between opposing sealing surfaces.

Abstract: A laparoscopic bipolar electrosurgical instrument for sealing tissue includes a handle having an elongated tube affixed thereto. The tube includes first and second jaw members having electrically conductive sealing surfaces attached to a distal end thereof which are movable from a first position for approximating tissue to a second position for grasping tissue therebetween. The handle includes a fixed handle and a handle which is movable relative to the fixed handle to effect movement of the jaw members from the first position to the second position for grasping tissue. The jaw members connect to a source of electrosurgical energy such that the opposable sealing surfaces are capable of conducting electrosurgical energy through tissue held therebetween. A stop is included for maintaining a minimum separation distance between opposing sealing surfaces. A ratchet is also included to maintain a closure force in the range of about 7 kg/cm2 to about 13 kg/cm2 between opposing sealing surfaces.

Abstract: A laparoscopic bipolar electrosurgical instrument can apply a large closure force between its jaws without damaging the small yoke assembly.

Abstract: A laparoscopic bipolar electrosurgical instrument can apply a large closure force between its jaw without damaging the small yoke assembly.

Abstract: A forceps for clamping, grasping, manipulating, coagulating/desiccating and cutting tissue includes a shaft portion having a proximal end, a distal end and first and second jaw members pivotally attached to the distal end of the shaft by a pivot assembly. Each of the jaw members including an opposing inner facing surface having a plurality of wave forms disposed thereon for engaging tissue therebetween. The wave forms disposed on the inner facing surface of the second jaw member being complimentary to the wave forms on the inner facing surface of first jaw member. A handle portion having an actuating assembly is attached to the proximal end of the shaft for imparting movement of the first and second jaw members from a first open position wherein the jaw members are disposed in spaced relation relative to one another to a second clamping position wherein the jaw members cooperate to grasp tissue therebetween.

Abstract: A endoscopic bipolar forceps for clamping and sealing tissue includes first and second jaw members pivotally attached in opposing relation relative to one another which are movable from a first open position wherein the jaw members are disposed in spaced relation relative to one another to a second clamping position wherein the jaw members cooperate to grasp tissue therebetween. A drive rod assembly connects each of the jaw members to a source of electrical energy such that the jaw members are capable of conducting bipolar energy through the tissue held therebetween. A handle attached to the drive rod assembly imparts movement of the first and second jaw members from the first and second positions. At least one stop member is attached to the jaw members for controlling the distance between the jaw members. The forceps may also include a yoke member for insulating the jaw members from one another to prevent short circuiting of the forceps.

Abstract: A endoscopic bipolar forceps for clamping and sealing tissue includes first and second jaw members pivotally attached in opposing relation relative to one another which are movable from a first open position wherein the jaw members are disposed in spaced relation relative to one another to a second clamping position wherein the jaw members cooperate to grasp tissue therebetween. A drive rod assembly connects each of the jaw members to a source of electrical energy such that the jaw members are capable of conducting bipolar energy through the tissue held therebetween. A handle attached to the drive rod assembly imparts movement of the first and second jaw members from the first and second positions. At least one stop member is attached to the jaw members for controlling the distance between the jaw members. The forceps may also include a yoke member for insulating the jaw members from one another to prevent short circuiting of the forceps.

Abstract: A endoscopic bipolar forceps for clamping and sealing tissue includes first and second jaw members pivotally attached in opposing relation relative to one another which are movable from a first open position wherein the jaw members are disposed in spaced relation relative to one another to a second clamping position wherein the jaw members cooperate to grasp tissue therebetween. A drive rod assembly connects each of the jaw members to a source of electrical energy such that the jaw members are capable of conducting bipolar energy through the tissue held therebetween. A handle attached to the drive rod assembly imparts movement of the first and second jaw members from the first and second positions. At least one stop member is attached to the jaw members for controlling the distance between the jaw members. The forceps may also include a yoke member for insulating the jaw members from one another to prevent short circuiting of the forceps.

Abstract: A laparoscopic bipolar electrosurgical instrument can apply a large closure force between its jaw without damaging the small yoke assembly.

Abstract: A laparoscopic bipolar electrosurgical instrument for sealing tissue includes a handle having an elongated tube affixed thereto. The tube includes first and second jaw members having electrically conductive sealing surfaces attached to a distal end thereof which are movable from a first position for approximating tissue to a second position for grasping tissue therebetween. The handle includes a fixed handle and a handle which is movable relative to the fixed handle to effect movement of the jaw members from the first position to the second position for grasping tissue. The jaw members connect to a source of electrosurgical energy such that the opposable sealing surfaces are capable of conducting electrosurgical energy through tissue held therebetween. A stop is included for maintaining a minimum separation distance between opposing sealing surfaces. A ratchet is also included to maintain a closure force in the range of about 7 kg/cm2 to about 13 kg/cm2 between opposing sealing surfaces.

Abstract: A bipolar electrosurgical instrument for clamping, grasping, manipulating, and sealing tissue includes first and second shafts each having a jaw member extending from a distal end thereof and a handle disposed at a proximal end thereof. The handle being operable to effect movement of the jaw members relative to one another from a first position wherein the jaw members are disposed in spaced relation relative to one another to a second position wherein the jaw members cooperate to grasp tissue therebetween. The bipolar instrument is connectable to a source of electrical energy having a first electrical potential connected to one of the jaw members and a second electrical potential connected to the other of the jaw members such that the jaw members are capable of selectively conducting energy through tissue held therebetween to effect a seal. Both the first and second electrical potentials are transmitted to the jaw members through the first shaft.

Abstract: A endoscopic bipolar forceps for clamping and sealing tissue includes first and second jaw members pivotally attached in opposing relation relative to one another which are movable from a first open position wherein the jaw members are disposed in spaced relation relative to one another to a second clamping position wherein the jaw members cooperate to grasp tissue therebetween. A drive rod assembly connects each of the jaw members to a source of electrical energy such that the jaw members are capable of conducting bipolar energy through the tissue held therebetween. A handle attached to the drive rod assembly imparts movement of the first and second jaw members from the first and second positions. At least one stop member is attached to the jaw members for controlling the distance between the jaw members. The forceps may also include a yoke member for insulating the jaw members from one another to prevent short circuiting of the forceps.

Abstract: A laparoscopic bipolar electrosurgical instrument can apply a large closure force between its jaws without damaging the small yoke assembly.

Abstract: A laparoscopic bipolar electrosurgical instrument can apply a large closure force between its jaws without damaging the small yoke assembly.

Abstract: A clamping force mechanism and its method of use with electrosurgery allow a user to seal and/or join patient's particular vascular tissue; the mechanism is elongate with user and patient ends. An actuator is at the user end and the effectors are at the patient end. Each effector has a face of an area to contact the particular vascular tissue. A lost motion connection transfers user actuation to the effectors to hold a predetermined clamping force during electrosurgical tissue sealing. A yielding member in the loss motion connection clamps the particular tissue between the faces with a predetermined force. The yielding member is a spring, slip clutch or hydraulic coupling possibly near the actuator. An active electrode is carried on one end effector and a return electrode contacts the tissue so an electrosurgical energy supply connected thereacross delivers energy therebetween. A feedback circuit responds to parameters of energy delivered to tissue.